Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,238,067	T→C	S253P (TCT→CCT)	ygjR →	putative oxidoreductase YgjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,238,067	0	T	C	100.0%	166.5 / NA	47	S253P (TCT→CCT)	ygjR	putative oxidoreductase YgjR
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/28); total (19/28)

GGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGATGAGCATCTGGTGGA  >  NC_000913/3237932‑3238197

ggcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGa                                                                                                                                <  1:881281/140‑1 (MQ=255)

                     gtggtgATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGt                                                                                                           >  2:579010/1‑140 (MQ=255)

                              gATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTgg                                                                                                  >  2:519045/1‑140 (MQ=255)

                               aTTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCGGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGc                                                                                                 >  1:59718/1‑140 (MQ=255)

                                ttACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCa                                                                                                >  2:88166/1‑140 (MQ=255)

                                   cGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGcc                                                                                             <  2:604366/140‑1 (MQ=255)

                                        aTTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATg                                                                                        >  2:646938/1‑140 (MQ=255)

                                         tttCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGc                                                                                       <  2:34016/140‑1 (MQ=255)

                                          ttCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCa                                                                                      >  2:161146/1‑140 (MQ=255)

                                               cGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAtc                                                                                 >  1:91833/1‑140 (MQ=255)

                                                         cagcaCTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGcc                                                                       >  2:92224/1‑140 (MQ=255)

                                                          agcaCTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg                                                                      <  2:488858/140‑1 (MQ=255)

                                                             aCTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   <  1:191806/140‑1 (MQ=255)

                                                             aCTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   <  1:161146/140‑1 (MQ=255)

                                                             aCTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   <  1:635750/140‑1 (MQ=255)

                                                             aCTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   <  1:646938/140‑1 (MQ=255)

                                                              cTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAGAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   <  2:57095/139‑1 (MQ=255)

                                                              cTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAGAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   >  1:57095/1‑139 (MQ=255)

                                                              cTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagc                                                                  >  1:158605/1‑140 (MQ=255)

                                                              cTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   >  1:583176/1‑139 (MQ=255)

                                                              cTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag                                                                   <  2:583176/139‑1 (MQ=255)

                                                               tCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGTAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagca                                                                 <  2:476462/140‑1 (MQ=255)

                                                               tCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagca                                                                 <  1:820084/140‑1 (MQ=255)

                                                               tCAAAAGTCAGTGATTCTGCCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGTAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGACCTCACCCCGCCgcagca                                                                 >  2:143160/1‑140 (MQ=255)

                                                                  aaaGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCatat                                                              <  2:449851/140‑1 (MQ=255)

                                                                        aGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagc                                                                  <  1:284762/130‑1 (MQ=255)

                                                                        aGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagc                                                                  >  2:284762/1‑130 (MQ=255)

                                                                         gTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATAcc                                                       <  1:366757/140‑1 (MQ=255)

                                                                         gTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATAcc                                                       <  1:579010/140‑1 (MQ=255)

                                                                                 gTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTa                                               <  2:107487/140‑1 (MQ=255)

                                                                                    cTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTa                                               <  2:223276/137‑1 (MQ=255)

                                                                                    cTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTa                                               >  1:223276/1‑137 (MQ=255)

                                                                                           gcgagATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCagag                                     <  1:88166/140‑1 (MQ=255)

                                                                                            cgagATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGc                                    <  2:192658/140‑1 (MQ=255)

                                                                                            cgagATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGc                                    >  2:59782/1‑140 (MQ=255)

                                                                                             gagaTTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCt                                   <  2:91833/140‑1 (MQ=255)

                                                                                                    aGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCt                            <  2:26161/140‑1 (MQ=255)

                                                                                                      ggCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTAc                          >  1:437450/1‑140 (MQ=255)

                                                                                                       gCGAAGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTAcc                         <  2:437450/140‑1 (MQ=255)

                                                                                                           aGCAGGATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCtgg                     >  1:214409/1‑140 (MQ=255)

                                                                                                                gATCGCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGAt                >  1:162254/1‑140 (MQ=255)

                                                                                                                    gCTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGATGAGc            <  1:519045/140‑1 (MQ=255)

                                                                                                                     cTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCtggt                    <  2:74609/131‑1 (MQ=255)

                                                                                                                     cTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCtggt                    >  1:74609/1‑131 (MQ=255)

                                                                                                                     cTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGATGAGc            <  2:106125/139‑1 (MQ=255)

                                                                                                                     cTGGTGATTGAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGATGAGc            >  1:106125/1‑139 (MQ=255)

                                                                                                                              gAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGATGAGCATCTGGTGGa  <  2:214409/140‑1 (MQ=255)

                                                                                                                              gAAAAACTGCCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGATGAGCATCTGGTGGa  <  2:158605/140‑1 (MQ=255)

GGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTAATACCATGCTCTACGAAGCAGAGCTGTTCGCTACCCTGGTGGATGAGCATCTGGTGGA  >  NC_000913/3237932‑3238197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A