Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,254,459	G→A	V47V (GTG→GTA)	yhaK →	bicupin‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,254,459	0	G	A	100.0%	153.2 / NA	45	V47V (GTG→GTA)	yhaK	bicupin‑related protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/22); total (23/22)

TACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCT  >  NC_000913/3254327‑3254593

tacCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGccc                                                                                                                                 >  1:52246/1‑140 (MQ=255)

     gAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGt                                                                                                                            >  1:724382/1‑140 (MQ=255)

              ggCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCttt                                                                                                                   <  1:148044/140‑1 (MQ=255)

                     tgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCcgc                                                                                                            >  2:132058/1‑140 (MQ=255)

                     tgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCcgc                                                                                                            >  1:701827/1‑140 (MQ=255)

                     tgGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCcgc                                                                                                            >  1:515425/1‑140 (MQ=255)

                         cAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAAc                                                                                                        <  1:369682/140‑1 (MQ=255)

                             cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                                    >  1:870917/1‑140 (MQ=255)

                             cAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                                    >  1:330375/1‑140 (MQ=255)

                             cAGACTACGGACGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTAt                                                                                                    >  1:790062/1‑140 (MQ=255)

                                 cTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCa                                                                                                <  2:515425/140‑1 (MQ=255)

                                    cGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAg                                                                                             >  2:529867/1‑140 (MQ=255)

                                        tGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGa                                                                                         <  1:132058/140‑1 (MQ=255)

                                          gTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGata                                                                                       >  2:158386/1‑140 (MQ=255)

                                          gTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGata                                                                                       >  2:63261/1‑140 (MQ=255)

                                           ttGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGatat                                                                                      >  1:215191/1‑140 (MQ=255)

                                                           cTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtg                                                                      >  2:568023/1‑140 (MQ=255)

                                                               ttCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtggatg                                                                  <  1:158386/140‑1 (MQ=255)

                                                                tCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAAt                                                                               >  1:529585/1‑126 (MQ=255)

                                                                tCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAAt                                                                               <  2:529585/126‑1 (MQ=255)

                                                                 ccTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtggatgga                                                                >  2:168749/1‑140 (MQ=255)

                                                                 ccTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCtggatgga                                                                >  1:450888/1‑140 (MQ=255)

                                                                  cTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGgag                                                               <  1:294421/140‑1 (MQ=255)

                                                                     tGGACACTACTTCTACCCTAAATTGTTAGGCTATGCCTCCCTGCGAGTGCTTAACCAGGAAGCACTGGCCCCAGGCGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGCGATTCTGGATGGAGAAg                                                            >  2:333491/1‑140 (MQ=255)

                                                                     tGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAg                                                            <  2:450888/140‑1 (MQ=255)

                                                                       gACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCa                                                          <  2:369018/140‑1 (MQ=255)

                                                                       gACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCa                                                          <  1:568023/140‑1 (MQ=255)

                                                                          actactTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCagag                                                       <  2:870917/140‑1 (MQ=255)

                                                                                   aCCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGAt                                              >  1:765020/1‑140 (MQ=255)

                                                                                      cGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGc                                           >  1:799677/1‑140 (MQ=255)

                                                                                      cGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGc                                           >  2:865357/1‑140 (MQ=255)

                                                                                          aTTGTAAGGTTATG‑CTCCCCGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                                      <  1:116669/140‑1 (MQ=255)

                                                                                           ttGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                                      <  1:168749/140‑1 (MQ=255)

                                                                                           ttGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAgg                                      <  1:434390/140‑1 (MQ=255)

                                                                                                aGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATc                                 <  1:175502/140‑1 (MQ=255)

                                                                                                aGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATc                                 <  1:865357/140‑1 (MQ=255)

                                                                                                           cccTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGcc                      <  1:529867/140‑1 (MQ=255)

                                                                                                            ccTGCGTGTGCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCa                     >  1:416254/1‑140 (MQ=255)

                                                                                                                  tgtgCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCg               <  2:464460/140‑1 (MQ=255)

                                                                                                                  tgtgCTTAACCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCG‑ACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCgg              >  1:625743/1‑140 (MQ=255)

                                                                                                                          aCCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACTTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAg                    <  1:636078/127‑1 (MQ=255)

                                                                                                                          aCCAGGAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACTTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAg                    >  2:636078/1‑127 (MQ=255)

                                                                                                                            cAGGAAGTACTGG‑CCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTtg    <  2:774064/140‑1 (MQ=255)

                                                                                                                              ggAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTtgc   >  1:65799/1‑140 (MQ=255)

                                                                                                                               gAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTtgct  <  2:416254/140‑1 (MQ=255)

                                                                                                                               gAAGTACTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTtgct  <  1:63261/140‑1 (MQ=255)

TACCCGAACTGCCAGGCAGTGTGGACAAGCAGACTACGGATGGTTGCAGGCCCGGTATACTTTTTCCTTTGGACACTACTTCGACCCGAAATTGTTAGGCTATGCCTCCCTGCGTGTGCTTAACCAGGAAGTGCTGGCCCCAGGTGCCGCCTTTCAGCCGCGAACCTATCCCAAAGTCGATATTTTAAATGTGATTCTGGATGGAGAAGCAGAGTATCGCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCT  >  NC_000913/3254327‑3254593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A