Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
163 |
52.9 |
1786516 |
99.3% |
1774010 |
139.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,305,886 |
T→C |
intergenic (‑69/+85) |
mtr ← / ← deaD |
tryptophan:H(+) symporter Mtr/ATP‑dependent RNA helicase DeaD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,305,886 | 0 | T | C | 95.3%
| 137.5
/ ‑3.8
| 43 | intergenic (‑69/+85) | mtr/deaD | tryptophan:H(+) symporter Mtr/ATP‑dependent RNA helicase DeaD |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (20/21); total (21/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGC > NC_000913/3305751‑3306024
|
tGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACaa > 1:835380/1‑140 (MQ=255)
cgccgATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAg > 1:624725/1‑140 (MQ=255)
gccgATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGa > 1:356261/1‑140 (MQ=255)
ccacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAg > 2:319824/1‑140 (MQ=255)
cgccgcCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGccc > 2:892153/1‑140 (MQ=255)
cgccgcCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGccc > 2:105666/1‑140 (MQ=255)
cgccgcCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGccc > 1:640512/1‑140 (MQ=255)
agcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCg > 1:204326/1‑134 (MQ=255)
agcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCg < 2:204326/134‑1 (MQ=255)
aCGGTGACGTTTGGGTGGTTGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCg < 1:413369/140‑1 (MQ=255)
ggTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCg < 1:757686/140‑1 (MQ=255)
gTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg < 2:356261/140‑1 (MQ=255)
gTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg < 1:157752/140‑1 (MQ=255)
gTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg < 2:640512/140‑1 (MQ=255)
gTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg < 1:314180/140‑1 (MQ=255)
ttaCGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc < 2:30552/138‑1 (MQ=255)
tGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc < 1:455596/140‑1 (MQ=255)
gACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCt < 1:105666/140‑1 (MQ=255)
gACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCt < 1:892153/140‑1 (MQ=255)
cGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGt < 2:624725/140‑1 (MQ=255)
gggTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatata < 2:835380/140‑1 (MQ=255)
gggTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatata < 2:203426/140‑1 (MQ=255)
gtTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg > 2:698186/1‑123 (MQ=255)
gtTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg < 1:698186/123‑1 (MQ=255)
gtTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCggg < 1:370902/122‑1 (MQ=255)
gtTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCggg > 2:370902/1‑122 (MQ=255)
ccATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTgtgt > 1:778585/1‑140 (MQ=255)
tGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCg > 2:88979/1‑140 (MQ=255)
gCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGtt < 2:538652/140‑1 (MQ=255)
gCGTTGTTATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAac > 2:370154/1‑140 (MQ=255)
ttATGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac < 1:88979/140‑1 (MQ=255)
aTGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAAcgacgacga > 2:190822/1‑140 (MQ=255)
tGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACgacgacgac < 1:190822/140‑1 (MQ=255)
tGCATTGCACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACgacgacgac < 2:778585/140‑1 (MQ=255)
cACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGa > 2:370216/1‑140 (MQ=255)
cACTGTACCAGTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGa > 2:440972/1‑140 (MQ=255)
gTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacga > 1:308555/1‑140 (MQ=255)
gTACACGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacga > 1:544943/1‑140 (MQ=255)
cacGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAcgc < 1:370216/140‑1 (MQ=255)
cacGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGAcgc < 1:440972/140‑1 (MQ=255)
cGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTGGAATCATCACGACGCgg > 2:709376/1‑140 (MQ=255)
cGAGCACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCgg > 1:451412/1‑140 (MQ=255)
gTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgc > 1:642579/1‑140 (MQ=255)
|
TGCCGCCGATAATCACCACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCT‑‑TCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGC > NC_000913/3305751‑3306024
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
