Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,408,942	T→C	Y446H (TAC→CAC)	panF →	pantothenate:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,408,942	0	T	C	94.9%	121.0 / ‑1.8	39	Y446H (TAC→CAC)	panF	pantothenate:Na(+) symporter
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (16/21); total (17/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

GGCTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCC  >  NC_000913/3408811‑3409069

ggCTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGc                                                                                                                         <  1:452003/140‑1 (MQ=255)

   tGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTc                                                                                                                      >  2:650691/1‑140 (MQ=255)

     gAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCa                                                                                                                    <  2:364821/140‑1 (MQ=255)

      aaGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCAc                                                                                                                   <  1:642752/140‑1 (MQ=255)

                   gtgGCCGCTGGTGCTGGGTCTTTACTCGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCt                                                                                                      <  2:790980/140‑1 (MQ=255)

                     ggCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCg                                                                                                    <  2:330099/140‑1 (MQ=255)

                             gTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTactact                                                                                            <  2:507739/140‑1 (MQ=255)

                              tGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTActacta                                                                                           >  1:244765/1‑140 (MQ=255)

                                   ggTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGttt                                                                                      <  2:244765/140‑1 (MQ=255)

                                             gggAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTc                                                                            >  2:94410/1‑140 (MQ=255)

                                                  cgcgCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGt                                                                       >  2:875790/1‑140 (MQ=255)

                                                   gcgcCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTc                                                                      >  1:810314/1‑140 (MQ=255)

                                                   gcgcCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTc                                                                      >  1:240698/1‑140 (MQ=255)

                                                     gccaacgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTggg                                                                    >  2:548030/1‑140 (MQ=255)

                                                     gccaacgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCgg                                                                    >  2:231112/1‑140 (MQ=255)

                                                         acgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAAc                                                                <  2:878821/140‑1 (MQ=255)

                                                          cgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAAcc                                                               >  1:435689/1‑140 (MQ=255)

                                                          cgccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACCACTAAGTTTGCTGGCTTTCCTGGTCGGAAAcc                                                               >  2:800134/1‑140 (MQ=255)

                                                            ccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGt                                                             <  2:445870/140‑1 (MQ=255)

                                                            ccaaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGt                                                             <  2:502688/140‑1 (MQ=255)

                                                             caaAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGtt                                                            <  1:548030/140‑1 (MQ=255)

                                                                   cgcgcTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTa                                                      >  1:872474/1‑140 (MQ=255)

                                                                     cgcTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACa                                                    >  1:491950/1‑140 (MQ=255)

                                                                            tGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTcc                                             >  1:786146/1‑140 (MQ=255)

                                                                                atgatCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCTTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCa                                         <  2:494032/140‑1 (MQ=255)

                                                                                atgatCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCa                                         <  1:231112/140‑1 (MQ=255)

                                                                                atgatCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCa                                         <  2:491950/140‑1 (MQ=255)

                                                                                         ggcggcGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGt                                <  2:872474/140‑1 (MQ=255)

                                                                                               gTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGac                          <  2:786146/140‑1 (MQ=255)

                                                                                               gTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGac                          <  1:629470/140‑1 (MQ=255)

                                                                                                  cTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGa                           <  1:891636/136‑1 (MQ=255)

                                                                                                  cTGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGa                           >  2:891636/1‑136 (MQ=255)

                                                                                                   tGTATGCCGTACTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGactact                      <  2:52056/140‑1 (MQ=255)

                                                                                                            tACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGataaataa             >  1:426507/1‑140 (MQ=255)

                                                                                                             aCTCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGAtaaataaa            >  2:156086/1‑140 (MQ=255)

                                                                                                               tCGCGACGCTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAaga          >  1:760857/1‑140 (MQ=255)

                                                                                                                       cTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGcc  <  2:426507/140‑1 (MQ=255)

                                                                                                                       cTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGcc  <  2:760857/140‑1 (MQ=255)

                                                                                                                       cTGAATATTCAGCACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGcc  <  1:650691/140‑1 (MQ=255)

GGCTGGAAGCCGTTTTCCTGTGGCCGCTGGTGCTGGGTCTTTACTGGGAACGCGCCAACGCCAAAGGCGCGCTAAGTGCGATGATCGTTGGCGGCGTGCTGTATGCCGTACTCGCGACGCTGAATATTCAGTACCTGGGCTTCCACCCTATCGTGCCCTCGTTACTACTAAGTTTGCTGGCTTTCCTGGTCGGAAACCGTTTCGGTACATCCGTCCCGCAAGCTACCGTTTTGACTACTGATAAATAAAGAGTTTTGCC  >  NC_000913/3408811‑3409069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A