Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,535,980	T→C	D202G (GAC→GGC)	ompR ←	DNA‑binding transcriptional dual regulator OmpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,535,980	0	T	C	100.0%	147.3 / NA	42	D202G (GAC→GGC)	ompR	DNA‑binding transcriptional dual regulator OmpR
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/21); total (21/21)

GTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCT  >  NC_000913/3535844‑3536115

gTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGa                                                                                                                                      <  2:706225/140‑1 (MQ=255)

                              agagCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGAc                                                                                                        >  1:865267/1‑140 (MQ=255)

                               gagCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGAcc                                                                                                       <  1:819926/140‑1 (MQ=255)

                                 gCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCAc                                                                                                     <  2:362416/140‑1 (MQ=255)

                                 gCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCAc                                                                                                     <  1:802509/140‑1 (MQ=255)

                                    gTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACggg                                                                                                  <  2:25271/140‑1 (MQ=255)

                                    gTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACggg                                                                                                  <  1:857558/140‑1 (MQ=255)

                                      ccGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCa                                                                                                <  2:865267/140‑1 (MQ=255)

                                               aGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTtcatc                                                                                       >  1:627091/1‑140 (MQ=255)

                                                    tAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCtt                                                                                  >  1:194455/1‑140 (MQ=255)

                                                     agcccagcccCCAGACGGTCGGAATGTACCGCGGATGCGCTGGATCTTCTTCCCCCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTa                                                                                 <  2:194455/140‑1 (MQ=255)

                                                      gCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTAt                                                                                <  2:111137/140‑1 (MQ=255)

                                                           gaccccagacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCgg                                                                           >  1:709041/1‑140 (MQ=255)

                                                                      gTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTc                                                                >  1:859476/1‑140 (MQ=255)

                                                                       tCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCa                                                               >  2:707417/1‑140 (MQ=255)

                                                                           aaTGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAcgcg                                                           <  1:872880/140‑1 (MQ=255)

                                                                           aaTGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAcgcg                                                           <  1:707417/140‑1 (MQ=255)

                                                                           aaTGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAcgcg                                                           <  1:375847/140‑1 (MQ=255)

                                                                             tGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGa                                                         <  2:353624/140‑1 (MQ=255)

                                                                               tAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATg                                                       >  2:841261/1‑140 (MQ=255)

                                                                                aaCGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATgg                                                      <  1:841261/140‑1 (MQ=255)

                                                                                aaCGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATgg                                                      <  2:627091/140‑1 (MQ=255)

                                                                                 aCGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGc                                                     >  2:418125/1‑140 (MQ=255)

                                                                                       aTGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCa                                               >  1:552744/1‑140 (MQ=255)

                                                                                       aTGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCa                                               >  1:330724/1‑140 (MQ=255)

                                                                                       aTGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCa                                               <  1:297365/140‑1 (MQ=255)

                                                                                         gcgcTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGt                                             <  2:859476/140‑1 (MQ=255)

                                                                                                      ttcCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCg                                >  2:471921/1‑140 (MQ=255)

                                                                                                       tcCACCATGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGc                               >  2:447434/1‑140 (MQ=255)

                                                                                                           ccaTGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAAc                           >  2:641223/1‑140 (MQ=255)

                                                                                                           ccaTGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAAc                           >  1:179835/1‑140 (MQ=255)

                                                                                                           ccaTGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAAc                           >  1:105465/1‑140 (MQ=255)

                                                                                                            caTGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGCGCCTTCAGTACCGCAAACt                          >  2:657077/1‑140 (MQ=255)

                                                                                                              tGCGGCGCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCa                        >  2:555558/1‑140 (MQ=255)

                                                                                                               gcggcgCAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCAc                       <  2:709041/140‑1 (MQ=255)

                                                                                                                   cgcAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCt                   >  1:717652/1‑140 (MQ=255)

                                                                                                                   cgcAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCt                   >  2:579974/1‑140 (MQ=255)

                                                                                                                   cgcAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCt                   >  1:183369/1‑140 (MQ=255)

                                                                                                                    gcAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTg                  <  1:418125/140‑1 (MQ=255)

                                                                                                                    gcAGACGCGAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTg                  <  1:447434/140‑1 (MQ=255)

                                                                                                                           cgAAATCTGCACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCg           <  2:690693/140‑1 (MQ=255)

                                                                                                                                    cACGCCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCt  >  1:117110/1‑140 (MQ=255)

GTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCGCAAACTCACCGCTGGTGAGCGGCATCGGCT  >  NC_000913/3535844‑3536115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A