Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,538,348	G→C	A322P (GCC→CCC)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,538,348	0	G	C	100.0%	141.3 / NA	40	A322P (GCC→CCC)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (17/23); total (17/23)

AAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCA  >  NC_000913/3538211‑3538479

aaGGCGTTGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGccc                                                                                                                                   >  1:687949/1‑140 (MQ=255)

    cGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTg                                                                                                                               <  1:108332/140‑1 (MQ=255)

         tgAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGa                                                                                                                          <  2:23053/140‑1 (MQ=255)

         tgAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGa                                                                                                                          <  2:250934/140‑1 (MQ=255)

           aGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCGATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACt                                                                                                                        >  1:526657/1‑140 (MQ=255)

           aGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACt                                                                                                                        <  2:567383/140‑1 (MQ=255)

           aGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACt                                                                                                                        <  2:268139/140‑1 (MQ=255)

                gACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTg                                                                                                                   <  1:349310/140‑1 (MQ=255)

                  cGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCa                                                                                                                 >  1:568516/1‑140 (MQ=255)

                       cgcATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGAt                                                                                                            <  2:687949/140‑1 (MQ=255)

                             aaGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCt                                                                                                      <  2:370356/140‑1 (MQ=255)

                             aaGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCt                                                                                                      <  1:863477/140‑1 (MQ=255)

                             aaGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCt                                                                                                      <  2:250470/140‑1 (MQ=255)

                                  gCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATc                                                                                                 <  2:737416/140‑1 (MQ=255)

                                   cTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATcc                                                                                                >  2:607694/1‑140 (MQ=255)

                                     gATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCgg                                                                                              <  1:750638/140‑1 (MQ=255)

                                       tGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGt                                                                                            <  1:607694/140‑1 (MQ=255)

                                           tCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGc                                                                                        >  1:117908/1‑140 (MQ=255)

                                           tCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGc                                                                                        >  1:662442/1‑140 (MQ=255)

                                               gAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTAc                                                                                    <  2:662442/140‑1 (MQ=255)

                                                   ccGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTggg                                                                                >  1:699347/1‑140 (MQ=255)

                                                    cGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTAc                                                                                    >  2:708498/1‑135 (MQ=255)

                                                    cGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTAc                                                                                    <  1:708498/135‑1 (MQ=255)

                                                      aaCTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTa                                                                             >  2:123480/1‑140 (MQ=255)

                                                           aTGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGt                                                                        >  2:846667/1‑140 (MQ=255)

                                                                     tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTc                                                              >  1:90013/1‑140 (MQ=255)

                                                                     tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTc                                                              >  1:651038/1‑140 (MQ=255)

                                                                     tGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTc                                                              >  2:535487/1‑140 (MQ=255)

                                                                         cgAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATg                                                          >  2:549478/1‑140 (MQ=255)

                                                                           aaCGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGcc                                                        <  1:794944/140‑1 (MQ=255)

                                                                                    aaGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCaaa                                               <  2:117908/140‑1 (MQ=255)

                                                                                               aTCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGcgac                                    <  2:19930/140‑1 (MQ=255)

                                                                                                       cTTTGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCa                            <  1:535487/140‑1 (MQ=255)

                                                                                                         ttGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCAtt                          <  2:90013/140‑1 (MQ=255)

                                                                                                          tGCCCGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGGTGCCCCTGGCAAAATGGGGGCGGCCGACACCAttt                         >  2:807803/1‑140 (MQ=255)

                                                                                                            cccGTAACCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTAc                       <  2:472461/140‑1 (MQ=255)

                                                                                                               gTAACCTGCACGATCTGCTGATGGCGACCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCg                    <  2:618808/140‑1 (MQ=255)

                                                                                                                  aCCTGCACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGcac                 <  1:549478/140‑1 (MQ=255)

                                                                                                                       cACGATCTGCTGATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCgg            >  1:534270/1‑140 (MQ=255)

                                                                                                                             ctgctgATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGc      <  1:846667/140‑1 (MQ=255)

                                                                                                                               gctgATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGATGCCACTGGCAAACCGGGGGGGGCCGACACCATTTACCCGCCCCCCCGACAAGccc    >  2:189597/1‑139 (MQ=255)

                                                                                                                                 tgATGGCGCCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGGCGGTGCCCCCGGCAAACTGGTGGGGGCCGACACCATTTACCCCCACACCGGGCAGGCCGcc  >  2:720779/1‑139 (MQ=255)

AAGGCGTGGTGAGCTGGACGTGGCGCATCAAGGTGCTGATGCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCCACTGGCAAACTGGTGGCGACCGACACCATTTACCCGCACACCGGACAGGCCGCA  >  NC_000913/3538211‑3538479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A