Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,541,362 T→C V319A (GTG→GCG)  feoB → Fe(2(+)) transporter FeoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,541,3620TC100.0% 250.3 / NA 74V319A (GTG→GCG) feoBFe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/52);  total (22/52)

GTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGTGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCT  >  NC_000913/3541226‑3541497
                                                                                                                                        |                                                                                                                                        
gTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTCCCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCa                                                                                                                                       <  1:1387212/140‑1 (MQ=255)
gTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCa                                                                                                                                       <  1:391976/140‑1 (MQ=255)
 tAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCAt                                                                                                                                      >  1:1032424/1‑140 (MQ=255)
  ggATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATg                                                                                                                                     <  1:1320423/139‑1 (MQ=255)
  aGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATg                                                                                                                                     <  1:671348/140‑1 (MQ=255)
  aGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATg                                                                                                                                     <  2:1031412/140‑1 (MQ=255)
  aGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATg                                                                                                                                     <  1:1303711/140‑1 (MQ=255)
   gATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACCGCCGCTGTTTTACCTTCGCTCCGTGGCGCTATTTGGGCATgg                                                                                                                                    >  2:1121859/1‑140 (MQ=255)
   gATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATgg                                                                                                                                    >  1:450385/1‑140 (MQ=255)
    aTAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGt                                                                                                                                   <  2:253104/140‑1 (MQ=255)
    aTAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGt                                                                                                                                   <  1:804467/140‑1 (MQ=255)
             tGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTTCAGCCGCTGTTTGACGTCGGCTCCCGGGCGCTATTTGCGCATGGTATTTAATgg                                                                                                                          >  2:131273/1‑140 (MQ=255)
             tGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCa                                                                                                                                       >  2:443479/1‑127 (MQ=255)
             tGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCa                                                                                                                                       <  1:443479/127‑1 (MQ=255)
             tGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATgg                                                                                                                          >  2:979902/1‑140 (MQ=255)
              gCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGGGGTTCAGCCGCTGTTTGACGCCGGCTCCGTGGCGCTATTTGCGCATGGTTTTCAATggc                                                                                                                         >  2:1320423/1‑139 (MQ=255)
               cTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGAt                                                                                                                        <  1:1121859/140‑1 (MQ=255)
                    ccGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCt                                                                                                                   <  2:1246647/140‑1 (MQ=255)
                    ccGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCt                                                                                                                   <  2:1038729/140‑1 (MQ=255)
                      gTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTac                                                                                                                 <  2:632711/140‑1 (MQ=255)
                       tttCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTaca                                                                                                                <  1:700292/140‑1 (MQ=255)
                        ttCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTacac                                                                                                               <  1:153826/140‑1 (MQ=255)
                         tCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTTACGTCGGCTCCCTGGCGCTATTTGCGCATGGTATTCAATGGATTTGCTacacc                                                                                                              >  2:868768/1‑139 (MQ=255)
                            tCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTc                                                                                                           <  1:979902/140‑1 (MQ=255)
                            tCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTCGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTc                                                                                                           <  2:1032424/140‑1 (MQ=255)
                             cGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTcc                                                                                                          <  2:1108931/140‑1 (MQ=255)
                             cGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTcc                                                                                                          <  2:253241/140‑1 (MQ=255)
                             cGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTcc                                                                                                          <  1:47513/140‑1 (MQ=255)
                               gTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTcccc                                                                                                        >  2:152773/1‑138 (MQ=255)
                                tCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACt                                                                                                       >  2:1339773/1‑140 (MQ=255)
                                              tctTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTg                                                                                        <  2:1328747/140‑1 (MQ=255)
                                                         tACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCCGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCt                                                                             <  2:637000/140‑1 (MQ=255)
                                                          aCCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTc                                                                            >  2:624102/1‑140 (MQ=255)
                                                          aCCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGGATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTc                                                                            >  1:1220135/1‑140 (MQ=255)
                                                          aCCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCCTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTc                                                                            >  1:407338/1‑140 (MQ=255)
                                                            cTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGc                                                                          >  1:520752/1‑140 (MQ=255)
                                                             tGATGTTCCTGCTGGCTATCAACATCGGCTGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGcc                                                                         <  1:70837/140‑1 (MQ=255)
                                                             tGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGcc                                                                         <  1:338848/140‑1 (MQ=255)
                                                              gATGTTCCTGCTGGCTATCAACATCGGCGGGGGGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTTCGCATGGGATTCAATGGATTGGCTACACGCTCCCCCTCCCCGA‑CTGGCTGACTATCTTCCTCcccc                                                                        >  2:338848/1‑140 (MQ=255)
                                                              gATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGccc                                                                        <  1:679594/140‑1 (MQ=255)
                                                              gATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGccc                                                                        <  2:28548/140‑1 (MQ=255)
                                                              gATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCCCCGCGGCGGTATTTGCGCATGGGATTTAATGGATTTGCTACACCCTCCCCTTCCCGGG‑CTGGCTTGCCATCTTCCTCGccc                                                                        >  2:679594/1‑140 (MQ=255)
                                                                   tCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGc                                                                   >  1:966323/1‑140 (MQ=255)
                                                                   tCCTGCTGGCTATCAACATCGGCAGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGc                                                                   >  2:605139/1‑140 (MQ=255)
                                                                    ccTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGCCGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGccc                                                                        <  2:1265469/134‑1 (MQ=255)
                                                                    ccTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGccc                                                                        >  1:1265469/1‑134 (MQ=255)
                                                                    ccTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGcc                                                                  >  2:799695/1‑140 (MQ=255)
                                                                    ccTGCTGGCTATCAACATCGGCGGGGCGTTACAACCGCCGTTTGACGTCGGCTCCGAGGGGGTATTTGCGCATGGTATTCAATGGAGTGGCTAAACACTCCACTTCCCGGACCGGGCTGA‑TATCCTCCTCGCCCAgtgac                                                                  >  2:119905/1‑136 (MQ=255)
                                                                     ctgctgGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGGTCCGTTGCGCTATTTTCGGATGGTATTTAAGGGGTTGGGTACACGCTCCACGTCCCGGG‑CTTGCTGACTATCTTCCTCCCCCACGGCCt                                                                 >  2:835645/1‑140 (MQ=255)
                                                                       gctgGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTgg                                                               >  1:825766/1‑140 (MQ=255)
                                                                       gctgGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTgg                                                               >  2:116468/1‑140 (MQ=255)
                                                                          ggCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTTACGTCGGCTCCGTGGGGCTATTTGCGCATGGGATTCAATGGATTGGGTACACGCCCCCCTTCCCGGA‑CTGGGTGGCTATCTTCCTCGCCCCGGGCCTggggg                                                            >  2:1101768/1‑138 (MQ=255)
                                                                          ggCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTggtgg                                                            >  1:836159/1‑137 (MQ=255)
                                                                          ggCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTg                                                            >  1:595115/1‑140 (MQ=255)
                                                                           gCTATCAACATCGGCGGGGGGTTACAACCGCTGTTTTACGTCGGCTCCGTGGGGCTATTTGCGCATGGGATTCAATTGATTTGCTACACGCTCCACTTCCCCGA‑CTGGCTGACTATCTTCCTCGCCCAGGGGCCGGGTgg                                                           >  2:756483/1‑140 (MQ=255)
                                                                            cTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTggc                                                          <  1:1101768/140‑1 (MQ=255)
                                                                            cTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTggc                                                          <  2:465989/140‑1 (MQ=255)
                                                                            cTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTggc                                                          <  1:624102/140‑1 (MQ=255)
                                                                            cTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTggc                                                          >  1:465989/1‑140 (MQ=255)
                                                                            cTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTggc                                                          <  1:721702/140‑1 (MQ=255)
                                                                              aTCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTggcgg                                                        <  1:646243/140‑1 (MQ=255)
                                                                               tCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTCGCGGCATTTGCGCATGGGATTCAATGGATTGGCCTCACGCCCCCCTTTCCCGA‑CAGGCTGGCTATTTTTCTTACCCAAGGCCTGGGTggcggg                                                       >  2:920250/1‑139 (MQ=255)
                                                                                     tCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAac                                                 <  2:876173/140‑1 (MQ=255)
                                                                                     tCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAac                                                 >  1:366034/1‑140 (MQ=255)
                                                                                     tCGGCGGGGCGTTACAGCCGCTGTTTGACGACGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAac                                                 >  1:599912/1‑140 (MQ=255)
                                                                                      cggcggGGCGTTACAGCCGCTGTTTGAAGTCGGCTCCGTGGCGCTATTTGCGCATGGGATTCAATGGATTGGCTACACGCCCCACTTCCCCGA‑CTGGCTGACTATCTTCCTCGCCCCGGGCCTGGGGGGCGGCATTAaca                                                >  2:723239/1‑140 (MQ=255)
                                                                                           gggCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGtg                                           <  1:489958/140‑1 (MQ=255)
                                                                                           gggCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGGATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCGTTAACACCGtg                                           >  2:875229/1‑140 (MQ=255)
                                                                                                        gCTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGc                              >  2:834253/1‑140 (MQ=255)
                                                                                                         cTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGcc                             >  1:1305309/1‑140 (MQ=255)
                                                                                                         cTGTTTGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGcc                             <  1:116468/140‑1 (MQ=255)
                                                                                                             ttGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAg                         <  2:1276727/140‑1 (MQ=255)
                                                                                                              tGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGa                        <  1:131273/140‑1 (MQ=255)
                                                                                                              tGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGa                        >  2:1232471/1‑140 (MQ=255)
                                                                                                              tGACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGa                        <  2:499310/140‑1 (MQ=255)
                                                                                                               gACGTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGAt                       <  1:920250/140‑1 (MQ=255)
                                                                                                                  gacGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTgg                    <  2:599912/138‑1 (MQ=255)
                                                                                                                  gTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTgg                    <  1:756483/140‑1 (MQ=255)
                                                                                                                  gTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTgg                    <  1:1216989/140‑1 (MQ=255)
                                                                                                                  gTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTgg                    <  1:1339773/140‑1 (MQ=255)
                                                                                                                  gTCGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTgg                    <  2:1305309/140‑1 (MQ=255)
                                                                                                                    cGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCa                  <  1:799695/140‑1 (MQ=255)
                                                                                                                    cGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCa                  <  1:868768/140‑1 (MQ=255)
                                                                                                                    cGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCa                  <  2:595115/140‑1 (MQ=255)
                                                                                                                    cGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCa                  <  2:966323/140‑1 (MQ=255)
                                                                                                                    cGGCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCa                  <  2:520752/140‑1 (MQ=255)
                                                                                                                      gCTCCGTGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCatg                >  1:181670/1‑140 (MQ=255)
                                                                                                                            tGGCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTAc          >  1:591663/1‑140 (MQ=255)
                                                                                                                             ggCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTccc         <  1:1232471/140‑1 (MQ=255)
                                                                                                                             ggCGCTATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTAcc         <  1:835645/140‑1 (MQ=255)
                                                                                                                                  tATTTGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTc    <  2:1278141/140‑1 (MQ=255)
                                                                                                                                    tttGCGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCct  >  1:835527/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTAGATAAAATCGTGCTCAACCGTTTCCTCGGTCTGCCGATTTTCCTCTTTGTGATGTACCTGATGTTCCTGCTGGCTATCAACATCGGCGGGGCGTTACAGCCGCTGTTTGACGTCGGCTCCGTGGCGCTATTTGTGCATGGTATTCAATGGATTGGCTACACGCTCCACTTCCCGGA‑CTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCT  >  NC_000913/3541226‑3541497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A