Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,546,735 T→C G59G (GGT→GGC)  gntT → high‑affinity gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,546,7350TC95.2% 195.4 / ‑1.6 63G59G (GGT→GGC) gntThigh‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (31/29);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTAC  >  NC_000913/3546598‑3546865
                                                                                                                                         |                                                                                                                                  
ttgCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAg                                                                                                                                  <  2:371438/140‑1 (MQ=255)
  gCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGcc                                                                                                                                >  1:144232/1‑140 (MQ=255)
  gCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGcc                                                                                                                                <  1:839582/140‑1 (MQ=255)
  gCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCATGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGcc                                                                                                                                >  2:733901/1‑140 (MQ=255)
    tCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCtt                                                                                                                              >  2:51957/1‑140 (MQ=255)
       tgatgaTCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGcc                                                                                                                           <  2:447836/140‑1 (MQ=255)
       tgatgaTCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGcc                                                                                                                           <  2:402576/140‑1 (MQ=255)
       tgatgaTCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGcc                                                                                                                           <  2:479269/140‑1 (MQ=255)
       tgatgaTCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGcc                                                                                                                           <  1:291225/140‑1 (MQ=255)
             tCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGcc                                                                                                                           >  1:567827/1‑134 (MQ=255)
             tCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGcc                                                                                                                           <  2:567827/134‑1 (MQ=255)
                gCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATAGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATg                                                                                                                  >  2:383401/1‑140 (MQ=255)
                 cTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAACCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATgg                                                                                                                 >  2:825619/1‑140 (MQ=255)
                   tCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGt                                                                                                               >  2:712443/1‑140 (MQ=255)
                     aaaaTGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGttt                                                                                                             <  2:360620/140‑1 (MQ=255)
                     aaaaTGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGttt                                                                                                             <  1:810832/140‑1 (MQ=255)
                     aaaaTGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGttt                                                                                                             <  2:91070/140‑1 (MQ=255)
                     aaaaTGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGttt                                                                                                             <  2:816649/140‑1 (MQ=255)
                             cGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                                                                                     <  2:144232/140‑1 (MQ=255)
                               gCTTCATCGCTCTCGTCCTCGTGGCGCGTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGGCCTGATCATGGGGTTTGGCGCCATg                                                                                                   >  2:396217/1‑140 (MQ=255)
                                   cATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                                                                               >  2:396069/1‑140 (MQ=255)
                                     tCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                                                                             >  2:741650/1‑140 (MQ=255)
                                     tCGCTCTCGTCCTCATGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                                                                             <  1:733901/140‑1 (MQ=255)
                                          ctcGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                                                                                        >  1:858257/1‑140 (MQ=255)
                                          ctcGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                                                                                        >  2:384861/1‑140 (MQ=255)
                                           tcGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGGTTTGGCGCAATGCTGGGCAAAATg                                                                                       >  2:617626/1‑140 (MQ=255)
                                             gTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                                                                                     >  1:764036/1‑140 (MQ=255)
                                                   gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                                                                               >  2:852351/1‑140 (MQ=255)
                                                     ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGGGGGACGCGCCGCAACCTTGCCCTGGTCATGGGTTTTAGCGCCATGCTGGGCTAAATGCTGGCAGACt                                                                             >  2:354237/1‑140 (MQ=255)
                                                       cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc                                                                           >  2:739823/1‑140 (MQ=255)
                                                         cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg                                                                         >  2:540906/1‑140 (MQ=255)
                                                         cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg                                                                         <  1:197952/140‑1 (MQ=255)
                                                         cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg                                                                         >  2:523046/1‑140 (MQ=255)
                                                         cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg                                                                         <  1:86457/140‑1 (MQ=255)
                                                           tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg                                                                       <  1:523046/140‑1 (MQ=255)
                                                            gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg                                                                      >  1:276629/1‑140 (MQ=255)
                                                                 tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac                                                                 <  1:396069/140‑1 (MQ=255)
                                                                     ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACg                                                             >  1:782072/1‑140 (MQ=255)
                                                                       aaTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAc                                                              >  1:6903/1‑137 (MQ=255)
                                                                       aaTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAc                                                              <  2:6903/137‑1 (MQ=255)
                                                                             aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGcca                                                     >  1:492608/1‑140 (MQ=255)
                                                                                  tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACg                                                >  1:299401/1‑140 (MQ=255)
                                                                                   gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTAt                                                          <  2:782444/129‑1 (MQ=255)
                                                                                   gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTAt                                                          >  1:782444/1‑129 (MQ=255)
                                                                                   gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGc                                               >  2:191001/1‑140 (MQ=255)
                                                                                    ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCt                                              <  2:408248/140‑1 (MQ=255)
                                                                                          gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGc                                        >  2:462495/1‑140 (MQ=255)
                                                                                          gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGc                                        >  2:109419/1‑140 (MQ=255)
                                                                                          gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGc                                        >  1:821793/1‑140 (MQ=255)
                                                                                                gTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAAtt                                  <  1:825619/140‑1 (MQ=255)
                                                                                                  tATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTg                                <  2:764036/140‑1 (MQ=255)
                                                                                                  tATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTg                                <  1:462495/140‑1 (MQ=255)
                                                                                                    ttGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGt                              >  1:179954/1‑140 (MQ=255)
                                                                                                      ggCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTaa                            <  2:821793/140‑1 (MQ=255)
                                                                                                         tCCATCAAAGCCGGTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTaaaaa                         >  1:141024/1‑140 (MQ=255)
                                                                                                                     ggTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGGGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTg             <  1:383401/140‑1 (MQ=255)
                                                                                                                     ggTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGTGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTg             >  1:765703/1‑140 (MQ=255)
                                                                                                                     ggTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTg             <  1:109419/140‑1 (MQ=255)
                                                                                                                     ggTGTCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTg             >  2:393265/1‑140 (MQ=255)
                                                                                                                      gtgtCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTATAAAACACATCCAGTgg            <  2:694559/140‑1 (MQ=255)
                                                                                                                       tgtCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTggg           <  1:372016/140‑1 (MQ=255)
                                                                                                                       tgtCGGCGGGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTggg           >  2:392887/1‑140 (MQ=255)
                                                                                                                          cggcggGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCgg        <  1:741650/140‑1 (MQ=255)
                                                                                                                          cggcggGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCgg        <  1:852351/140‑1 (MQ=255)
                                                                                                                            gcggGACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCggtg      >  1:422304/1‑140 (MQ=255)
                                                                                                                                gACGCTCGGCAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTAc  >  1:236796/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                  
TTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGGGACGCTCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTAC  >  NC_000913/3546598‑3546865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A