Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,845,834 A→G V445A (GTA→GCA)  uhpT ← hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,845,8340AG100.0% 85.2 / NA 26V445A (GTA→GCA) uhpThexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/10);  total (16/10)

TGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGC  >  NC_000913/3845700‑3845966
                                                                                                                                      |                                                                                                                                    
tGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTgccgcc                                                                                                                                 >  2:625902/1‑140 (MQ=255)
           cGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCCCCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCAt                                                                                                                      <  1:874381/140‑1 (MQ=255)
               ggAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAgg                                                                                                                  <  1:350533/140‑1 (MQ=255)
               ggAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAgg                                                                                                                  <  1:802545/140‑1 (MQ=255)
                   gTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGa                                                                                                              >  2:470018/1‑140 (MQ=255)
                             aataaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCCCCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAAt                                                                                                    <  1:844808/140‑1 (MQ=255)
                              ataaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGAt                                                                                                             <  2:563434/130‑1 (MQ=255)
                              ataaAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGAt                                                                                                             >  1:563434/1‑130 (MQ=255)
                                    aaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGTATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCgcggcg                                                                                             >  1:324373/1‑140 (MQ=255)
                                    aaaGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCgcggcg                                                                                             <  1:625902/140‑1 (MQ=255)
                                        cccGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGatat                                                                                         >  1:343143/1‑140 (MQ=255)
                                             cGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAgc                                                                                    >  2:442324/1‑140 (MQ=255)
                                                  tGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTTCGTTCTTCCATTGCCGCCACTATCGCCATCAGGTAGATACAACCAATCGCGGCGATATCCAGcgcgcc                                                                               >  2:550864/1‑138 (MQ=255)
                                                                           tttATGCCACTGTCAACTGCTGATTTTTTTTCTCGCGGCGGTTTTTGCTTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAgg                                                      <  2:805384/140‑1 (MQ=255)
                                                                               tGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGa                                                  >  1:694464/1‑140 (MQ=255)
                                                                                gCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGaa                                                 >  1:424034/1‑140 (MQ=255)
                                                                                gCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTCCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGaa                                                 <  1:834716/140‑1 (MQ=255)
                                                                                   aCTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATAc                                              >  1:536361/1‑140 (MQ=255)
                                                                                    cTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATAcc                                             >  2:825220/1‑140 (MQ=255)
                                                                                    cTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATAcc                                             >  1:805384/1‑140 (MQ=255)
                                                                                    cTGTCAACTGCTGAATTTTTTTCTAGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATAcc                                             >  2:416251/1‑140 (MQ=255)
                                                                                       tCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGc                                          >  1:736414/1‑140 (MQ=255)
                                                                                         aaCTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGt                                        >  2:103200/1‑140 (MQ=255)
                                                                                               tgAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATc                                  >  1:747129/1‑140 (MQ=255)
                                                                                                   tttttttCTCGCGGCGGATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCa                              >  2:402585/1‑140 (MQ=255)
                                                                                                                 gggATTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGAcc                <  2:307244/139‑1 (MQ=255)
                                                                                                                    aTTTTGCGTTCTTCCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTaa             <  2:424034/140‑1 (MQ=255)
                                                                                                                              cttcCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAg   <  1:529243/140‑1 (MQ=255)
                                                                                                                               ttcCATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGc  <  1:416251/140‑1 (MQ=255)
                                                                                                                               tcccATTGCCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGc  <  2:654755/138‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TGCCTTCACTACGCTGGAAGTCACGGAATAATAAAAAAAGCCCGGCGTCATGCCGGGCAAAAGTCACCAGTTACGTTTATGCCACTGTCAACTGCTGAATTTTTTTCTCGCGGCGGATTTTGCGTTCTTCCATTACCGCCACTATCGCCATCAGGCAGATACAACCAATCGCGGCGATATCCAGCGCGGCGAAGGTGCCTGCCCAGCCGGTAAGGCCGAATACCGGCGTCCCATCGGCAATCATTCCCAGACCTAACTTGGCAAAGC  >  NC_000913/3845700‑3845966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A