Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,002,366	A→G	N314S (AAC→AGC)	corA →	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,002,366	0	A	G	95.0%	124.0 / ‑1.7	40	N314S (AAC→AGC)	corA	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (21/17); total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGC  >  NC_000913/4002229‑4002502

gccgACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCt                                                                                                                                        >  2:77642/1‑140 (MQ=255)

     cGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTg                                                                                                                                   <  1:25362/140‑1 (MQ=255)

                  cAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGagag                                                                                                                      >  2:428786/1‑140 (MQ=255)

                    gCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCg                                                                                                                    <  1:29490/140‑1 (MQ=255)

                        tGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTgg                                                                                                                >  2:4518/1‑140 (MQ=255)

                        tGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTgg                                                                                                                >  2:252453/1‑140 (MQ=255)

                          gCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCt                                                                                                              <  1:98249/140‑1 (MQ=255)

                              gAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGt                                                                                                          >  1:574556/1‑140 (MQ=255)

                                    tGAGTTTATGCCGGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCt                                                                                                    <  1:421409/140‑1 (MQ=255)

                                         ttATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGcc                                                                                               <  1:799020/140‑1 (MQ=255)

                                           aTGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTTAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCAc                                                                                             >  1:836831/1‑140 (MQ=255)

                                              ccAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTg                                                                                          <  1:767273/140‑1 (MQ=255)

                                              ccAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTg                                                                                          <  1:304860/140‑1 (MQ=255)

                                                     tGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCa                                                                                   <  1:251467/140‑1 (MQ=255)

                                                       aaGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCaaa                                                                                 <  2:582074/140‑1 (MQ=255)

                                                                  cGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAg                                                                                >  2:201459/1‑130 (MQ=255)

                                                                  cGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAg                                                                                <  1:201459/130‑1 (MQ=255)

                                                                             gcgcGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCttt                                                           >  2:597277/1‑140 (MQ=255)

                                                                               gcgATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTa                                                         >  2:262373/1‑140 (MQ=255)

                                                                                  aTTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACct                                                      <  1:6724/140‑1 (MQ=255)

                                                                                  aTTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACct                                                      >  2:472713/1‑140 (MQ=255)

                                                                                       cTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTTAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTtc                                                 <  2:836831/140‑1 (MQ=255)

                                                                                           aTGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCttt                                                  >  1:346554/1‑135 (MQ=255)

                                                                                           aTGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCttt                                                  <  2:346554/135‑1 (MQ=255)

                                                                                             gATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAg                                           >  2:593830/1‑140 (MQ=255)

                                                                                              aTCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGa                                          <  1:472713/140‑1 (MQ=255)

                                                                                                ccTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAc                                        >  2:239982/1‑140 (MQ=255)

                                                                                                ccTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAc                                        >  1:316146/1‑140 (MQ=255)

                                                                                                ccTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAc                                        >  1:118088/1‑140 (MQ=255)

                                                                                                 cTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAAcc                                       >  2:16366/1‑140 (MQ=255)

                                                                                                  tCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCa                                      >  2:609928/1‑140 (MQ=255)

                                                                                                         ccTGGCACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTc                               >  2:123618/1‑140 (MQ=255)

                                                                                                              cACCGTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGtt                          <  1:244750/140‑1 (MQ=255)

                                                                                                                  gTATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                      >  1:467529/1‑140 (MQ=255)

                                                                                                                   tATCTGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAg                     <  2:574556/140‑1 (MQ=255)

                                                                                                                       tGTACTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAAc                 <  1:252453/140‑1 (MQ=255)

                                                                                                                           cTTTAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAg             <  1:593830/140‑1 (MQ=255)

                                                                                                                             ttAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgc           >  2:292398/1‑140 (MQ=255)

                                                                                                                              tAAGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgct          >  1:642094/1‑140 (MQ=255)

                                                                                                                                aGCGGAAGAGCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGgctgc        >  2:273750/1‑140 (MQ=255)

                                                                                                                                    gaagagCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                      <  1:622322/122‑1 (MQ=255)

                                                                                                                                    gaagagCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACa                      >  2:622322/1‑122 (MQ=255)

                                                                                                                                      agagCTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGc  <  1:262373/140‑1 (MQ=255)

GCCGACGCTCGTTGCTTCCAGCTATGGCATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGCTTCGGCTACCCTGGCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAAAAAACGAGAGCGGTGGCTTAGTCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCATAGAACCATTTGTTCGTGTTAACAGCAACATAGGCTGCTACGGC  >  NC_000913/4002229‑4002502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A