Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,132,127	C→T	L765L (CTG→TTG)	metL →	fused aspartate kinase/homoserine dehydrogenase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,132,127	0	C	T	97.2%	116.1 / ‑4.1	36	L765L (CTG→TTG)	metL	fused aspartate kinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (19/16); total (19/17)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGCTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTG  >  NC_000913/4131999‑4132261

tGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGAt                                                                                                                             <  1:649290/140‑1 (MQ=255)

     cAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGt                                                                                                                        >  1:856343/1‑140 (MQ=255)

         ggCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATa                                                                                                                            >  1:34161/1‑132 (MQ=255)

         ggCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATa                                                                                                                            <  2:34161/132‑1 (MQ=255)

         ggCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCttt                                                                                                                    >  1:111368/1‑140 (MQ=255)

         ggCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCttt                                                                                                                    >  1:310935/1‑140 (MQ=255)

             ggAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGGTGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCa                                                                                                                >  1:73210/1‑140 (MQ=255)

                                gggCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATcg                                                                                             <  2:85263/140‑1 (MQ=255)

                                     ggTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATa                                                                                        <  2:856343/140‑1 (MQ=255)

                                      gTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATaa                                                                                       <  1:193145/140‑1 (MQ=255)

                                      gTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGCTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATaa                                                                                       <  2:315746/140‑1 (MQ=255)

                                        gctgcGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAAcc                                                                                     <  2:85429/140‑1 (MQ=255)

                                        gctgcGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAAcc                                                                                     <  2:111368/140‑1 (MQ=255)

                                               tACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGt                                                                              <  2:732469/140‑1 (MQ=255)

                                                aCGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTg                                                                             >  1:587293/1‑140 (MQ=255)

                                                aCGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTg                                                                             >  2:850732/1‑140 (MQ=255)

                                                      cgcgTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCcgc                                                                       >  2:386028/1‑140 (MQ=255)

                                                                 gCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGgcgc                                                            <  2:731822/140‑1 (MQ=255)

                                                                     aCGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTggg                                                        <  2:587293/140‑1 (MQ=255)

                                                                       ggTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGgcg                                                      <  1:786355/140‑1 (MQ=255)

                                                                         tAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGcgcg                                                    <  2:73210/140‑1 (MQ=255)

                                                                                 gtgtAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCAcc                                            >  2:730285/1‑140 (MQ=255)

                                                                                  tgtAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCAccg                                           >  2:368259/1‑140 (MQ=255)

                                                                                   gtAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCAccgc                                          <  2:376558/140‑1 (MQ=255)

                                                                                   gtAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCAccgc                                          >  1:824664/1‑140 (MQ=255)

                                                                                            gAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGAt                                 >  2:365834/1‑140 (MQ=255)

                                                                                             aaGCGGTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGAtt                                <  1:365834/140‑1 (MQ=255)

                                                                                                 ggTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGt                            >  2:334401/1‑140 (MQ=255)

                                                                                                  gTGCGTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTc                           >  2:839157/1‑140 (MQ=255)

                                                                                                      gTGAAGATCATCCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGat                       >  1:379994/1‑140 (MQ=255)

                                                                                                               atcCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCgg              >  2:381885/1‑140 (MQ=255)

                                                                                                                tcCGTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGc             >  1:357207/1‑140 (MQ=255)

                                                                                                                   gTTGGCATCTCTGTTGCCGGGCGATAACGTCTTTGCTATCGAAAGCCGCTGGTATCGCGATAGCCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGGCGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTgg          <  2:75167/140‑1 (MQ=255)

                                                                                                                   gTTGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTgg          <  2:379994/140‑1 (MQ=255)

                                                                                                                     tGGCATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGca        <  2:824664/140‑1 (MQ=255)

                                                                                                                        cATCACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAg     >  2:254443/1‑140 (MQ=255)

                                                                                                                           cACTGTTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAgttg  >  1:703612/1‑140 (MQ=255)

TGGTGCAACGGCTGGAAGCGGCCCGCGAAATGGGGCTGGTGCTGCGCTACGTGGCGCGTTTCGATGCCAACGGTAAAGCGCGTGTAGGCGTGGAAGCGGTGCGTGAAGATCATCCGTTGGCATCACTGCTGCCGTGCGATAACGTCTTTGCCATCGAAAGCCGCTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTG  >  NC_000913/4131999‑4132261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A