Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,132,292	T→C	intergenic (+25/‑324)	metL → / → metF	fused aspartate kinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,132,292	0	T	C	100.0%	183.5 / NA	52	intergenic (+25/‑324)	metL/metF	fused aspartate kinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/26); total (26/26)

CTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTG  >  NC_000913/4132162‑4132426

cTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGtt                                                                                                                               >  2:682876/1‑140 (MQ=255)

             aaCCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTAt                                                                                                                    >  1:1160773/1‑138 (MQ=255)

             aaCCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTAt                                                                                                                    <  2:1160773/138‑1 (MQ=255)

              aCCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGt                                                                                                                 >  1:919591/1‑140 (MQ=255)

                 ctctGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTc                                                                                                              <  1:823556/140‑1 (MQ=255)

                   ctGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTa                                                                                                            <  1:269714/140‑1 (MQ=255)

                   ctGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTa                                                                                                            >  2:1210981/1‑140 (MQ=255)

                     ggTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAAt                                                                                                          <  2:97926/140‑1 (MQ=255)

                      gTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATg                                                                                                         >  2:353169/1‑140 (MQ=255)

                      gTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATg                                                                                                         >  2:974462/1‑140 (MQ=255)

                       tGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGa                                                                                                        <  1:682876/140‑1 (MQ=255)

                          tCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAat                                                                                                     >  2:121179/1‑140 (MQ=255)

                          tCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAat                                                                                                     >  1:174275/1‑140 (MQ=255)

                              cgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGaa                                                                                                       >  2:820151/1‑134 (MQ=255)

                              cgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGaa                                                                                                       <  1:820151/134‑1 (MQ=255)

                              cgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatatat                                                                                                 >  1:717417/1‑140 (MQ=255)

                              cgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatatat                                                                                                 >  2:651678/1‑140 (MQ=255)

                                gACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatatat                                                                                                 >  2:1217038/1‑138 (MQ=255)

                                gACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatatat                                                                                                 <  1:1217038/138‑1 (MQ=255)

                                 gccTGGCGCTGGGCGCGACGTCGCCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGa                                                                                              <  2:454532/139‑1 (MQ=255)

                                  ccTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGaa                                                                                             <  2:1161672/140‑1 (MQ=255)

                                  ccTGGCGCTGGGCGCGACGGCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGaa                                                                                             <  1:904143/140‑1 (MQ=255)

                                   cTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAAt                                                                                            <  1:1210981/140‑1 (MQ=255)

                                     ggCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTa                                                                                          >  1:851942/1‑140 (MQ=255)

                                      gcgcTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTAt                                                                                         >  1:841908/1‑140 (MQ=255)

                                      gcgcTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTAt                                                                                         <  1:503/140‑1 (MQ=255)

                                      gcgcTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTAt                                                                                         >  1:174361/1‑140 (MQ=255)

                                           gggCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAg                                                                                    >  1:6445/1‑140 (MQ=255)

                                            ggCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAgg                                                                                   >  2:483196/1‑140 (MQ=255)

                                                            cgGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAg                                                                   <  2:174361/140‑1 (MQ=255)

                                                                gCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGtt                                                               <  1:483196/140‑1 (MQ=255)

                                                                     tCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTacga                                                          <  1:651678/140‑1 (MQ=255)

                                                                      cAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTacgac                                                         <  2:919591/140‑1 (MQ=255)

                                                                              tatCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCgg                                                 >  1:785880/1‑140 (MQ=255)

                                                                                 cAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCaa                                              <  2:998397/140‑1 (MQ=255)

                                                                                   aCCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCaaga                                            >  1:845769/1‑140 (MQ=255)

                                                                                      ggCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGc                                         <  1:682937/140‑1 (MQ=255)

                                                                                       gCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCa                                        >  1:733336/1‑140 (MQ=255)

                                                                                        cTGGCACAGTTGTTGTAATTTCAGAAATTTAAT‑ATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                      <  2:1145447/140‑1 (MQ=255)

                                                                                         tGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                      <  2:733336/140‑1 (MQ=255)

                                                                                              caGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTa                                 <  2:845769/140‑1 (MQ=255)

                                                                                                 ttgttgTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTAc                                <  1:72593/138‑1 (MQ=255)

                                                                                                 ttgttgTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTAc                                >  2:72593/1‑138 (MQ=255)

                                                                                                 ttgttgTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACAt                              >  1:983600/1‑140 (MQ=255)

                                                                                                  tgttgtAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATg                             <  2:6445/140‑1 (MQ=255)

                                                                                                       tAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAg                        <  2:717417/140‑1 (MQ=255)

                                                                                                           ttCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTta                    >  1:649919/1‑140 (MQ=255)

                                                                                                                aaaTTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATAttt               >  2:777172/1‑140 (MQ=255)

                                                                                                                     taataaTGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGt          <  2:649919/140‑1 (MQ=255)

                                                                                                                             cccGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGtttg  >  1:860384/1‑140 (MQ=255)

                                                                                                                             cccGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGtttg  >  2:895034/1‑140 (MQ=255)

                                                                                                                             cccGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGtttg  <  1:121179/140‑1 (MQ=255)

CTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTG  >  NC_000913/4132162‑4132426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A