Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,157,986	A→G	V379V (GTA→GTG)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,157,986	0	A	G	100.0%	127.1 / NA	37	V379V (GTA→GTG)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/24); total (13/24)

GCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATT  >  NC_000913/4157854‑4158121

gctggACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTcc                                                                                                                                  >  2:763582/1‑140 (MQ=255)

gctggACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTcc                                                                                                                                  >  2:601805/1‑140 (MQ=255)

 ctggACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCcg                                                                                                                                 <  2:448013/140‑1 (MQ=255)

       tgcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgc                                                                                                                           <  1:426784/140‑1 (MQ=255)

        gcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgcg                                                                                                                          <  2:804017/140‑1 (MQ=255)

        gcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgcg                                                                                                                          <  1:426877/140‑1 (MQ=255)

        gcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgcg                                                                                                                          <  1:494545/140‑1 (MQ=255)

        gcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgcg                                                                                                                          <  1:642497/140‑1 (MQ=255)

        gcctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgcg                                                                                                                          <  1:763582/140‑1 (MQ=255)

         cctgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgcgc                                                                                                                         <  1:601805/140‑1 (MQ=255)

           tgcATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCAc                                                                                                                       <  2:635961/140‑1 (MQ=255)

                 tGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATAtt                                                                                                                 <  1:811317/140‑1 (MQ=255)

                   gcggcgCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgc                                                                                                                           <  1:603457/128‑1 (MQ=255)

                   gcggcgCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGgc                                                                                                                           >  2:603457/1‑128 (MQ=255)

                       cgcTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTATGCCAACGCCACCCAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAg                                                                                                                             >  2:93597/1‑122 (MQ=255)

                       cgcTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTATGCCAACGCCACCCAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAg                                                                                                                             <  1:93597/122‑1 (MQ=255)

                       cgcTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGt                                                                                                           >  1:485208/1‑140 (MQ=255)

                           ggTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAg                                                                                                       <  2:485208/140‑1 (MQ=255)

                           ggTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAg                                                                                                       <  2:372337/140‑1 (MQ=255)

                              gCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCgg                                                                                                    <  1:682655/140‑1 (MQ=255)

                              gCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCgg                                                                                                    <  2:382623/140‑1 (MQ=255)

                                  gACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCggtggt                                                                                                >  2:346651/1‑140 (MQ=255)

                                      gCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGaa                                                                                            >  1:90385/1‑140 (MQ=255)

                                          tCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCa                                                                                        <  1:346651/140‑1 (MQ=255)

                                                 aaaCGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCa                                                                                 >  2:655435/1‑140 (MQ=255)

                                                      tCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCa                                                                            >  2:504053/1‑140 (MQ=255)

                                                        cACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCaaa                                                                          <  1:655435/140‑1 (MQ=255)

                                                        cACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCaaa                                                                          <  1:504053/140‑1 (MQ=255)

                                                        cACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCaaa                                                                          >  1:422167/1‑140 (MQ=255)

                                                                cAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGa                                                                  <  1:723985/140‑1 (MQ=255)

                                                                       cGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTg                                                           >  1:104703/1‑140 (MQ=255)

                                                                                       cgccaacgccaCCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGc                                           >  1:806377/1‑140 (MQ=255)

                                                                                                  ccGAACTGGCGGATTATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATtcagt                                <  2:422167/140‑1 (MQ=255)

                                                                                                                tATCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGa                  <  1:840509/140‑1 (MQ=255)

                                                                                                                 aTCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGaa                 <  2:104703/140‑1 (MQ=255)

                                                                                                                 aTCTGGTGGCGAAAGGCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGaa                 <  2:806377/140‑1 (MQ=255)

                                                                                                                                gCGTGCCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGAtt  >  1:438118/1‑140 (MQ=255)

GCTGGACTGCCTGCATATGGCGGCGCTGGTGCTGGACGGCATTCAGGTGAAACGTCCACGTTGCCAGGAAGCGGCTCAGCAGGGTTACGCCAACGCCACCGAACTGGCGGATTATCTGGTGGCGAAAGGCGTACCGTTCCGCGAGGCGCACCATATTGTTGGTGAAGCGGTGGTGGAAGCCATTCGTCAGGGCAAACCGCTGGAAGATCTGCCGCTCAGTGAGTTGCAGAAATTCAGTCAGGTGATTGACGAAGATGTCTATCCGATT  >  NC_000913/4157854‑4158121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A