Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,252,853	A→G	G116G (GGA→GGG)	ubiC →	chorismate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,252,853	0	A	G	100.0%	165.1 / NA	48	G116G (GGA→GGG)	ubiC	chorismate lyase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/26); total (22/26)

GTCTCGTTACTGGTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTGTTTTTACCG  >  NC_000913/4252718‑4252985

gTCTCGTTACTGGTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCt                                                                                                                                  <  2:662765/140‑1 (MQ=255)

gTCTCGTTACTGGTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCt                                                                                                                                  <  1:487042/140‑1 (MQ=255)

      ttACTGGTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGt                                                                                                                            >  2:863276/1‑140 (MQ=255)

        aCTGGTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTc                                                                                                                          <  1:712321/140‑1 (MQ=255)

          tGGTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTcac                                                                                                                        >  1:715503/1‑140 (MQ=255)

           ggTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTcaca                                                                                                                       <  2:432477/140‑1 (MQ=255)

              tACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCAcatca                                                                                                                    >  2:268990/1‑140 (MQ=255)

               aCGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCAcatcat                                                                                                                   <  1:165447/140‑1 (MQ=255)

               aCGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCAcatcat                                                                                                                   >  1:770894/1‑140 (MQ=255)

               aCGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCAcatcat                                                                                                                   <  1:380944/140‑1 (MQ=255)

                cGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACatcatc                                                                                                                  <  2:666155/140‑1 (MQ=255)

                   gAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGAc                                                                                                               <  2:769104/140‑1 (MQ=255)

                      aTTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACAtt                                                                                                            <  1:863276/140‑1 (MQ=255)

                      aTTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACAtt                                                                                                            <  1:38707/140‑1 (MQ=255)

                      aTTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACAtt                                                                                                            <  1:252741/140‑1 (MQ=255)

                                gtgCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGAc                                                                                                  <  1:458432/140‑1 (MQ=255)

                                gtgCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGAc                                                                                                  <  2:770894/140‑1 (MQ=255)

                                gtgCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGAc                                                                                                  <  1:885358/140‑1 (MQ=255)

                                 tgCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGTCGGAGCGGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACt                                                                                                 >  2:69557/1‑140 (MQ=255)

                                      aTGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGAc                                                                                                  >  1:197344/1‑134 (MQ=255)

                                      aTGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGAc                                                                                                  <  2:197344/134‑1 (MQ=255)

                                                 tGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCg                                                                                 <  2:715503/140‑1 (MQ=255)

                                                 tGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCg                                                                                 >  2:519734/1‑140 (MQ=255)

                                                 tGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCg                                                                                 <  1:165258/140‑1 (MQ=255)

                                                         cGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCg                                                                         <  1:108680/140‑1 (MQ=255)

                                                           gTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCggg                                                                       >  1:856511/1‑140 (MQ=255)

                                                                      gtTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCg                                                            >  2:448923/1‑140 (MQ=255)

                                                                               tCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCg                                                   >  2:361673/1‑140 (MQ=255)

                                                                                 aaCGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGcc                                                 <  2:856511/140‑1 (MQ=255)

                                                                                         gCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGTCATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTa                                         >  1:477765/1‑140 (MQ=255)

                                                                                         gCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTa                                         >  1:579001/1‑140 (MQ=255)

                                                                                           gggCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTACCCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAg                                       <  1:291274/140‑1 (MQ=255)

                                                                                             gCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCg                                     >  1:106493/1‑140 (MQ=255)

                                                                                             gCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCg                                     >  1:841700/1‑140 (MQ=255)

                                                                                               cGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGt                                   >  2:358259/1‑140 (MQ=255)

                                                                                                  aGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTaaa                                >  2:410850/1‑140 (MQ=255)

                                                                                                       gCGTTACAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCt                           >  2:374822/1‑140 (MQ=255)

                                                                                                            aCAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCt                           >  2:473813/1‑135 (MQ=255)

                                                                                                            aCAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCt                           <  1:473813/135‑1 (MQ=255)

                                                                                                            aCAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGc                      >  1:852242/1‑140 (MQ=255)

                                                                                                             cAAAAATTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCt                     <  1:519734/140‑1 (MQ=255)

                                                                                                               aaaaTTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATGGACATTAACCCGGGACTTTATTGAGACAGGCCGTGATGCCGGGCTGGGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTaa                   <  2:779781/140‑1 (MQ=255)

                                                                                                                 aaTTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAAc                               >  2:764466/1‑126 (MQ=255)

                                                                                                                 aaTTGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAAc                               <  1:764466/126‑1 (MQ=255)

                                                                                                                    tGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGaa              <  1:448923/140‑1 (MQ=255)

                                                                                                                    tGGGTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGaa              <  1:361673/140‑1 (MQ=255)

                                                                                                                       gTAAAACGCCGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTg           >  1:851801/1‑140 (MQ=255)

                                                                                                                               ccGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTGTTTTTAcc   <  2:851801/140‑1 (MQ=255)

                                                                                                                                cGTTAGGGCGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGTCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTGTTTTTACCg  >  2:751436/1‑140 (MQ=255)

GTCTCGTTACTGGTTACGTGAAATTTTGTTATGTGCCGATGGTGAACCGTGGCTTGCCGGTCGTACCGTCGTTCCTGTGTCAACGTTAAGCGGGCCGGAGCTGGCGTTACAAAAATTGGGTAAAACGCCGTTAGGACGCTATCTGTTCACATCATCGACATTAACCCGGGACTTTATTGAGATAGGCCGTGATGCCGGGCTGTGGGGGCGACGTTCCCGCCTGCGATTAAGCGGTAAACCGCTGTTGCTAACAGAACTGTTTTTACCG  >  NC_000913/4252718‑4252985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A