Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,471,590 G→A P269L (CCG→CTG)  pyrB ← aspartate carbamoyltransferase catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,471,5900GA100.0% 252.0 / NA 72P269L (CCG→CTG) pyrBaspartate carbamoyltransferase catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/37);  total (35/37)

TACAGTACCAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCGGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTCATGTACAGG  >  NC_000913/4471461‑4471721
                                                                                                                                 |                                                                                                                                   
tACAGTACCAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGCTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATg                                                                                                                           >  1:277943/1‑140 (MQ=255)
   aGTACCAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATgcag                                                                                                                        <  2:45633/140‑1 (MQ=255)
   aGTACCAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATgcag                                                                                                                        <  1:402281/140‑1 (MQ=255)
     tACCAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATgcagca                                                                                                                      >  1:19142/1‑140 (MQ=255)
     tACCAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATgcagca                                                                                                                      >  1:260320/1‑140 (MQ=255)
       ccAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACt                                                                                                                    >  1:388708/1‑140 (MQ=255)
          gATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTc                                                                                                                 >  2:707565/1‑140 (MQ=255)
           aTCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCa                                                                                                                >  1:59231/1‑140 (MQ=255)
            tCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCat                                                                                                               >  1:711392/1‑140 (MQ=255)
             cgcgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCata                                                                                                              <  1:643334/140‑1 (MQ=255)
               cgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATAtt                                                                                                            >  1:494770/1‑140 (MQ=255)
               cgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATAtt                                                                                                            >  1:378293/1‑140 (MQ=255)
               cgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATAtt                                                                                                            >  2:343477/1‑140 (MQ=255)
               cgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATAtt                                                                                                            >  2:429607/1‑140 (MQ=255)
               cgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATAtt                                                                                                            >  2:442951/1‑140 (MQ=255)
               cgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCATGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATgcag                                                                                                                        >  1:842164/1‑128 (MQ=255)
               cgATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCATGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATgcag                                                                                                                        <  2:842164/128‑1 (MQ=255)
                    cAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCtt                                                                                                       <  2:380318/140‑1 (MQ=255)
                      gAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTg                                                                                                     <  2:388708/140‑1 (MQ=255)
                      gAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTg                                                                                                     <  2:872163/140‑1 (MQ=255)
                       aaCCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTgg                                                                                                    >  2:50556/1‑140 (MQ=255)
                         ccAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCg                                                                                                  <  1:295574/140‑1 (MQ=255)
                          cAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGt                                                                                                 <  2:393116/140‑1 (MQ=255)
                            gTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTtg                                                                                               >  2:428153/1‑140 (MQ=255)
                            gTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTtg                                                                                               >  1:450805/1‑140 (MQ=255)
                                cAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGga                                                                                           <  1:425514/140‑1 (MQ=255)
                                       gCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCt                                                                                    >  2:222853/1‑140 (MQ=255)
                                           ggCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCg                                                                                      >  1:510155/1‑134 (MQ=255)
                                           ggCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCg                                                                                      <  2:510155/134‑1 (MQ=255)
                                           ggCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGgcg                                                                                >  2:273096/1‑140 (MQ=255)
                                           ggCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGgcg                                                                                >  1:273134/1‑140 (MQ=255)
                                           ggCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGgcg                                                                                >  1:373722/1‑140 (MQ=255)
                                           ggCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGgcg                                                                                >  1:757276/1‑140 (MQ=255)
                                           ggCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGgcg                                                                                >  1:526391/1‑140 (MQ=255)
                                              gagcgaAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCga                                                                             <  1:856024/140‑1 (MQ=255)
                                              gagcgaAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCga                                                                             <  1:273096/140‑1 (MQ=255)
                                                gcgaAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCgaag                                                                           >  2:544915/1‑140 (MQ=255)
                                                 cgaAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCgaaga                                                                          <  1:50556/140‑1 (MQ=255)
                                                                   cctgcTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACg                                                        <  2:829233/140‑1 (MQ=255)
                                                                      gctgGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTg                                                     <  1:41376/140‑1 (MQ=255)
                                                                      gctgGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTg                                                     <  2:450805/140‑1 (MQ=255)
                                                                             gTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACt                                              <  1:429607/140‑1 (MQ=255)
                                                                                  aGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGAc                                         >  2:734224/1‑140 (MQ=255)
                                                                                   ggCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACg                                        >  2:503129/1‑140 (MQ=255)
                                                                                        gtgGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTcc                                   >  2:115142/1‑140 (MQ=255)
                                                                                         tgGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCa                                  >  2:6077/1‑140 (MQ=255)
                                                                                              ttttATCAACATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGc                             >  1:474577/1‑140 (MQ=255)
                                                                                                     aaCATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCttttt                      >  2:826746/1‑140 (MQ=255)
                                                                                                      aCATCCGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTg                     <  2:277943/140‑1 (MQ=255)
                                                                                                           cGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCAcgc                >  2:84622/1‑140 (MQ=255)
                                                                                                           cGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCAcgc                <  2:526391/140‑1 (MQ=255)
                                                                                                           cGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCAcgc                <  2:873039/140‑1 (MQ=255)
                                                                                                           cGTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCAcgc                <  2:373722/140‑1 (MQ=255)
                                                                                                            gTCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGAGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCAcgcg               <  1:300891/140‑1 (MQ=255)
                                                                                                             tCGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCgg              >  1:133641/1‑140 (MQ=255)
                                                                                                              cGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGTGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  2:494770/140‑1 (MQ=255)
                                                                                                              cGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  1:101188/140‑1 (MQ=255)
                                                                                                              cGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  2:757276/140‑1 (MQ=255)
                                                                                                              cGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  1:813895/140‑1 (MQ=255)
                                                                                                              cGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  2:230121/140‑1 (MQ=255)
                                                                                                              cGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  2:273134/140‑1 (MQ=255)
                                                                                                              cGCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  2:378293/140‑1 (MQ=255)
                                                                                                               gCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGt            <  2:59231/140‑1 (MQ=255)
                                                                                                               gCAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGt            <  2:474577/140‑1 (MQ=255)
                                                                                                                cAATCTCATCAACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTc           <  1:428153/140‑1 (MQ=255)
                                                                                                                     tcatcaACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTCATGTa      <  2:19142/140‑1 (MQ=255)
                                                                                                                      catcaACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             >  2:719905/1‑132 (MQ=255)
                                                                                                                      catcaACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCggg             <  1:719905/132‑1 (MQ=255)
                                                                                                                        tcaACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTCATGTACAg   >  2:442343/1‑140 (MQ=255)
                                                                                                                         caACACGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTCATGTACAgg  <  2:295245/140‑1 (MQ=255)
                                                                                                                             acGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGACGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTCa          >  2:718670/1‑128 (MQ=255)
                                                                                                                             acGCAGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGACGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTCa          <  1:718670/128‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
TACAGTACCAGATCGCGATTCAGAACCAGTGCCAGTAACGCCTGGCGAGCGAAAATCCCGTTGCCTGCCTGCTGGAAGTACCAGGCGTGTGGCGTTTTATCAACATCCGTCGCAATCTCATCAACACGCGGCAGCGGATGCAGCACTTTCATATTGGCTTTGGCGTTGTGGAGATCGCTGGCGCGAAGAACAAACTGCGCTTTCACGTTGGCGTACTCGGACGGGTCCAGACGCTCTTTTTGCACGCGGGTCATGTACAGG  >  NC_000913/4471461‑4471721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A