Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
260 |
73.5 |
2514320 |
99.3% |
2496719 |
138.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,472,426 |
(A)8→9 |
coding (108/135 nt) |
pyrL ← |
pyrBI operon leader peptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,472,418 | 1 | . | A | 100.0%
| 162.4
/ NA
| 46 | C39F (TGC→TTC) | pyrL | pyrBI operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (27/19); total (27/19) |
GCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAA > NC_000913/4472281‑4472549
|
gCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑‑aa > 2:821212/1‑140 (MQ=255)
gCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCCCCTGACGCCTGGGC‑‑aaaaaaaaagc < 1:259850/140‑4 (MQ=255)
cGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑‑aaaaaaaaagcc > 1:1159380/1‑136 (MQ=255)
cGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑‑aaaaaaaaag > 2:561380/1‑136 (MQ=255)
cGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑‑aaaaaaaaag < 1:561380/138‑3 (MQ=255)
cTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGAtt < 2:138069/140‑1 (MQ=255)
tttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGc > 1:361686/1‑140 (MQ=255)
gTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAg < 1:58276/126‑1 (MQ=255)
gTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAg > 2:58276/1‑126 (MQ=255)
cACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAAc < 2:1159380/140‑1 (MQ=255)
aGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaa > 1:716606/1‑140 (MQ=255)
ggTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa > 2:389825/1‑140 (MQ=255)
aTCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGc > 2:422069/1‑140 (MQ=255)
aTCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGc > 2:412191/1‑140 (MQ=255)
tCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCa > 2:218955/1‑140 (MQ=255)
cgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGc > 2:881707/1‑140 (MQ=255)
cgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACACCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGc < 1:881707/140‑1 (MQ=255)
ggTCGTTTATGGTAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTc > 1:1030121/1‑140 (MQ=255)
ttATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCtttttt < 2:361686/140‑1 (MQ=255)
tGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAg > 2:449069/1‑140 (MQ=255)
ggAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGa < 1:422069/140‑1 (MQ=255)
ggAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGa < 1:389825/140‑1 (MQ=255)
aaaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAcg < 1:417479/140‑1 (MQ=255)
tGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCgg < 1:218955/140‑1 (MQ=255)
tttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACa < 1:812321/140‑1 (MQ=255)
tGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAAt > 2:980026/1‑140 (MQ=255)
taGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGa < 2:716606/140‑1 (MQ=255)
aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa > 2:524226/1‑140 (MQ=255)
aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa > 1:110967/1‑140 (MQ=255)
aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa < 2:360674/140‑1 (MQ=255)
gCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAac > 2:630857/1‑140 (MQ=255)
cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca > 2:104088/1‑140 (MQ=255)
cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca > 1:914291/1‑140 (MQ=255)
cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca > 1:1174278/1‑140 (MQ=255)
cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca > 1:706623/1‑140 (MQ=255)
cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca > 1:57814/1‑140 (MQ=255)
cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca > 1:282004/1‑140 (MQ=255)
gATTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca < 1:630857/140‑1 (MQ=255)
aTTAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacacac < 2:906599/140‑1 (MQ=255)
ttAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACt > 2:898716/1‑140 (MQ=255)
tAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGa > 2:175776/1‑132 (MQ=255)
tAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGa < 1:175776/132‑1 (MQ=255)
tAGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTg > 1:1094055/1‑140 (MQ=255)
aGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGa < 1:104088/140‑1 (MQ=255)
aGCCATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGa < 2:1094055/140‑1 (MQ=255)
cATCTTTTATCTCCTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAcc < 2:914291/140‑1 (MQ=255)
ccTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTccc > 2:221129/1‑140 (MQ=255)
ccTGACGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGG‑GATCAACGGGAAGAAAAACCGCAGGCCCGCGGCTTTTTTCAGACGGGGGAAGACCAAATGTCGAAAACACTTAAACCAACATCCTcccc > 2:399555/1‑139 (MQ=255)
aCGCCTGGGCAAAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGc > 2:409261/1‑140 (MQ=255)
aCGCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCa > 1:599908/1‑140 (MQ=255)
gCCTGGGCA‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCaaa < 1:821212/140‑1 (MQ=255)
|
GCTCTGGTTGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGC‑‑AAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAA > NC_000913/4472281‑4472549
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
