Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,473,717	C→T	A113T (GCC→ACC)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,473,717	0	C	T	100.0%	118.4 / NA	37	A113T (GCC→ACC)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/18); total (19/18)

GCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGAC  >  NC_000913/4473584‑4473849

gcCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATa                                                                                                                                <  1:68690/140‑1 (MQ=255)

       cTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCa                                                                                                                         <  1:907195/140‑1 (MQ=255)

        ttGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCAt                                                                                                                        <  2:162720/140‑1 (MQ=255)

              ggCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGTAGCATGAatat                                                                                                                  >  1:137001/1‑140 (MQ=255)

                cAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGc                                                                                                                          >  2:618602/1‑130 (MQ=255)

                cAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGc                                                                                                                          <  1:618602/130‑1 (MQ=255)

                cAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTg                                                                                                                >  1:333465/1‑140 (MQ=255)

                   aTTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGAtt                                                                                                             >  2:571788/1‑140 (MQ=255)

                   aTTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGAtt                                                                                                             >  2:45474/1‑140 (MQ=255)

                           gcggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGTAGCATGAATATTGATTTTGAAAAg                                                                                                     <  2:137001/140‑1 (MQ=255)

                           gcggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAg                                                                                                     <  1:571788/140‑1 (MQ=255)

                           gcggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAg                                                                                                     <  1:45474/140‑1 (MQ=255)

                           gcggcATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAg                                                                                                     <  2:814175/140‑1 (MQ=255)

                                        ccATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGaaa                                                                                                       >  2:244309/1‑125 (MQ=255)

                                        ccATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGaaa                                                                                                       <  1:244309/125‑1 (MQ=255)

                                            gCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCgg                                                                                    <  2:845850/140‑1 (MQ=255)

                                                      ggCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTc                                                                          >  1:70988/1‑140 (MQ=255)

                                                            cGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGc                                                                    <  2:478247/140‑1 (MQ=255)

                                                                 gCCATTCACGGAGCCGCTGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGc                                                               >  2:784942/1‑140 (MQ=255)

                                                                 gCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGc                                                               >  1:612581/1‑140 (MQ=255)

                                                                  ccATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGcc                                                              >  2:885945/1‑140 (MQ=255)

                                                                   cATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCa                                                             <  2:612581/140‑1 (MQ=255)

                                                                   cATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCa                                                             >  1:1017419/1‑140 (MQ=255)

                                                                     ttCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCaaa                                                           >  2:127778/1‑140 (MQ=255)

                                                                           gAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGcc                                                     <  1:639578/140‑1 (MQ=255)

                                                                           gAGCCCATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGcc                                                     <  2:209460/140‑1 (MQ=255)

                                                                                gatgatTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATAc                                                <  1:885945/140‑1 (MQ=255)

                                                                                 atgatTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATAcc                                               >  1:8849/1‑140 (MQ=255)

                                                                                        aGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCAttt                                        >  2:319439/1‑140 (MQ=255)

                                                                                        aGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCAttt                                        >  2:856543/1‑140 (MQ=255)

                                                                                            gcgcCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTacca                                    >  1:1018179/1‑140 (MQ=255)

                                                                                              gcccgccTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCTATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTaccacc                                  >  1:791250/1‑140 (MQ=255)

                                                                                                          gCATCTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAAt                      >  1:688294/1‑140 (MQ=255)

                                                                                                              cTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATgcgc                  <  2:688294/140‑1 (MQ=255)

                                                                                                              cTGCCGGGCGGCTTCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATgcgc                  >  2:246304/1‑140 (MQ=255)

                                                                                                                          ttCAACAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTacgaa      <  1:516214/140‑1 (MQ=255)

                                                                                                                              aCAGAGGTATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGAc  <  2:1018179/140‑1 (MQ=255)

GCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCATCTGCCGGGCGGCTTCAACAGAGGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGAC  >  NC_000913/4473584‑4473849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A