Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,545,520	T→C	V142A (GTC→GCC)	fimD →	type I fimbriae usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,545,520	0	T	C	96.2%	255.0 / ‑0.9	79	V142A (GTC→GCC)	fimD	type I fimbriae usher protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (38/38); total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

TACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGCCGGATTGCT  >  NC_000913/4545386‑4545655

tACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAgg                                                                                                                                    >  2:69618/1‑140 (MQ=255)

 aCGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGa                                                                                                                                   <  1:889769/140‑1 (MQ=255)

   gggCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACg                                                                                                                                 <  1:522764/140‑1 (MQ=255)

    ggCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTCCAGGACGc                                                                                                                                >  1:669512/1‑140 (MQ=255)

    ggCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGc                                                                                                                                >  2:29876/1‑140 (MQ=255)

    ggCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGc                                                                                                                                >  2:674249/1‑140 (MQ=255)

    ggCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGc                                                                                                                                >  1:157486/1‑140 (MQ=255)

      cGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTa                                                                                                                              >  2:848600/1‑140 (MQ=255)

           gTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTgcg                                                                                                                         <  2:225396/140‑1 (MQ=255)

              aaCAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCAt                                                                                                                      <  1:269126/140‑1 (MQ=255)

                     gATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATg                                                                                                               <  2:753760/140‑1 (MQ=255)

                      aTTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTg                                                                                                                           <  1:371503/127‑1 (MQ=255)

                      aTTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTg                                                                                                                           >  2:371503/1‑127 (MQ=255)

                              ctgcctgACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTCCAGGACGCTACTGCGCATCTGGATGTTGGTcagc                                                                                                      >  1:233617/1‑140 (MQ=255)

                               tgcctgACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTcagca                                                                                                     >  2:18080/1‑140 (MQ=255)

                               tgcctgACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTcagca                                                                                                     >  1:93131/1‑140 (MQ=255)

                               tgcctgACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTcagca                                                                                                     <  1:525476/140‑1 (MQ=255)

                               tgcctgACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTcagca                                                                                                     >  1:476260/1‑140 (MQ=255)

                               tgcctgACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATATTGGTcagca                                                                                                     >  2:421970/1‑140 (MQ=255)

                                gcctgACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTcagcag                                                                                                    >  2:814442/1‑140 (MQ=255)

                                    gACACGCGCGCAACTCGCCAGTATGGGGCTGAATATGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGAc                                                                                                <  1:368112/140‑1 (MQ=255)

                                    gACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCagcagc                                                                                                   >  1:575420/1‑137 (MQ=255)

                                    gACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCagcagc                                                                                                   <  2:575420/137‑1 (MQ=255)

                                    gACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGAc                                                                                                >  2:780323/1‑140 (MQ=255)

                                    gACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGAc                                                                                                <  1:114992/140‑1 (MQ=255)

                                    gACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCCCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGAc                                                                                                >  2:556070/1‑140 (MQ=255)

                                     acacGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACt                                                                                               >  2:394915/1‑140 (MQ=255)

                                      cacGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTg                                                                                              <  1:556070/140‑1 (MQ=255)

                                        cgcgcgCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGaa                                                                                            <  2:458348/140‑1 (MQ=255)

                                          cgcgcAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAAcc                                                                                          >  2:598302/1‑140 (MQ=255)

                                           gcgcAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCt                                                                                         >  2:185641/1‑140 (MQ=255)

                                           gcgcAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCt                                                                                         >  1:110837/1‑140 (MQ=255)

                                           gcgcAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCt                                                                                         >  1:751757/1‑140 (MQ=255)

                                              cAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGAc                                                                                      <  1:18080/140‑1 (MQ=255)

                                              cAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGAc                                                                                      <  2:191164/140‑1 (MQ=255)

                                                 cTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGAt                                                                                   <  1:848600/140‑1 (MQ=255)

                                                      cAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTc                                                                              <  1:674249/140‑1 (MQ=255)

                                                         tATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAgg                                                                           <  1:817924/140‑1 (MQ=255)

                                                         tATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAgg                                                                           <  2:157486/140‑1 (MQ=255)

                                                            ggggCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCAt                                                                        <  2:543247/140‑1 (MQ=255)

                                                            ggggCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCAt                                                                        <  2:476260/140‑1 (MQ=255)

                                                              ggCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCAttt                                                                      >  1:603266/1‑140 (MQ=255)

                                                                cTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTAt                                                                    <  1:814442/140‑1 (MQ=255)

                                                                cTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTAt                                                                    <  2:93131/140‑1 (MQ=255)

                                                                   aaTACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAg                                                                 >  2:409943/1‑140 (MQ=255)

                                                                   aaTACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAg                                                                 >  1:762859/1‑140 (MQ=255)

                                                                        ggCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATc                                                            >  1:499070/1‑140 (MQ=255)

                                                                          cTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgc                                                          >  2:95652/1‑140 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcg                                                       <  2:669512/140‑1 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAAt                                                             >  1:389860/1‑134 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAAt                                                             <  2:389860/134‑1 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcg                                                       >  1:112990/1‑140 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcg                                                       <  2:371743/140‑1 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcg                                                       >  1:371743/1‑140 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcg                                                       >  2:122334/1‑140 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcg                                                       >  1:454535/1‑140 (MQ=255)

                                                                             cTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcg                                                       >  1:678084/1‑140 (MQ=255)

                                                                              tGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcgc                                                      >  2:116733/1‑140 (MQ=255)

                                                                               gTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcgcg                                                     <  1:394915/140‑1 (MQ=255)

                                                                               gTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATcgcgcgcg                                                     <  2:603266/140‑1 (MQ=255)

                                                                                     ggTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTta                                               >  2:270502/1‑140 (MQ=255)

                                                                                       tATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTtata                                             <  2:640796/140‑1 (MQ=255)

                                                                                       tATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTtata                                             <  1:435136/140‑1 (MQ=255)

                                                                                       tATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTtata                                             <  1:29876/140‑1 (MQ=255)

                                                                                       tATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTtata                                             >  2:793202/1‑140 (MQ=255)

                                                                                               tgctgGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCGTTTATGAGTAATCGCGCGCGTGGTTATATTcctcct                                     <  2:110837/140‑1 (MQ=255)

                                                                                               tgctgGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTcctcct                                     <  1:856369/140‑1 (MQ=255)

                                                                                                 ctgGCGGATGATGCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGa                                   >  1:188457/1‑140 (MQ=255)

                                                                                                           atgCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGAt                         <  1:122334/140‑1 (MQ=255)

                                                                                                             gCCTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATcc                       <  1:409943/140‑1 (MQ=255)

                                                                                                              ccTGTGTGCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATccc                      <  2:454535/140‑1 (MQ=255)

                                                                                                                   gtgCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTAt                 <  2:188457/140‑1 (MQ=255)

                                                                                                                   gtgCCATTAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTAAATTCCTCCTGAGTTATGGGATCCCGGTAt                 <  1:295928/140‑1 (MQ=255)

                                                                                                                         ttAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGc           >  1:701139/1‑140 (MQ=255)

                                                                                                                         ttAACCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGc           >  2:86766/1‑140 (MQ=255)

                                                                                                                           aaCCACAATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGTCg         <  2:762859/140‑1 (MQ=255)

                                                                                                                              cacaATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGCCGGAt      >  1:464458/1‑140 (MQ=255)

                                                                                                                                caATGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGCCGGATTg    >  2:558452/1‑140 (MQ=255)

                                                                                                                                  aTGGCCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGCCGGATTGCt  <  2:223474/140‑1 (MQ=255)

TACGGGCGACAGTGAACAAGGGATTGTTCCCTGCCTGACACGCGCGCAACTCGCCAGTATGGGGCTGAATACGGCTTCTGTCGCCGGTATGAATCTGCTGGCGGATGATGCCTGTGTGCCATTAACCACAATGGTCCAGGACGCTACTGCGCATCTGGATGTTGGTCAGCAGCGACTGAACCTGACGATCCCTCAGGCATTTATGAGTAATCGCGCGCGTGGTTATATTCCTCCTGAGTTATGGGATCCCGGTATTAATGCCGGATTGCT  >  NC_000913/4545386‑4545655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A