Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,557,185 T→C Y65H (TAT→CAT)  iraD → anti‑adaptor protein IraD, inhibitor of sigma(S) proteolysis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,557,1850TC97.5% 269.2 / ‑2.7 80Y65H (TAT→CAT) iraDanti‑adaptor protein IraD, inhibitor of sigma(S) proteolysis
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (38/40);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

TCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCT  >  NC_000913/4557050‑4557321
                                                                                                                                       |                                                                                                                                        
tCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTg                                                                                                                                      >  1:817810/1‑140 (MQ=255)
 cGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGc                                                                                                                                     >  1:668788/1‑140 (MQ=255)
        tAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGc                                                                                                                              >  2:117927/1‑140 (MQ=255)
         aaaTCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCt                                                                                                                             <  1:122513/140‑1 (MQ=255)
                tATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAATCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCt                                                                                                                      >  2:766660/1‑140 (MQ=255)
                tATGCAGCGATCTCCTAACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCt                                                                                                                      >  2:723590/1‑140 (MQ=255)
                 aTGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTg                                                                                                                     <  1:117927/140‑1 (MQ=255)
                 aTGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTg                                                                                                                     <  1:394871/140‑1 (MQ=255)
                   gCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCa                                                                                                                   <  1:875976/140‑1 (MQ=255)
                   gCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCa                                                                                                                   <  2:668788/140‑1 (MQ=255)
                   gCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCa                                                                                                                   <  1:777747/140‑1 (MQ=255)
                   gCAGCGATCTCCTAACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCa                                                                                                                   <  1:723590/140‑1 (MQ=255)
                    cAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCAc                                                                                                                  <  1:397475/140‑1 (MQ=255)
                         aTCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTa                                                                                                             >  1:426913/1‑140 (MQ=255)
                          tctcCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTAt                                                                                                            >  1:629179/1‑140 (MQ=255)
                            tcctcACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTAttt                                                                                                          >  1:770127/1‑140 (MQ=255)
                             cctcACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTc                                                                                                         <  2:817810/140‑1 (MQ=255)
                                 aCTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGa                                                                                                     >  2:144733/1‑140 (MQ=255)
                                    ctTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGcc                                                                                                  <  2:572965/140‑1 (MQ=255)
                                      ttttAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCAc                                                                                                >  1:363763/1‑140 (MQ=255)
                                      ttttAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCAc                                                                                                >  1:339947/1‑140 (MQ=255)
                                             tctcCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgc                                                                                         >  2:466839/1‑140 (MQ=255)
                                               tcctcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgcgg                                                                                       <  1:11194/140‑1 (MQ=255)
                                               tcctcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgcgg                                                                                       >  2:11194/1‑140 (MQ=255)
                                               tcctcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgcgg                                                                                       >  2:323311/1‑140 (MQ=255)
                                                 ctcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgcggcg                                                                                     >  2:208430/1‑140 (MQ=255)
                                                 ctcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgcggcg                                                                                     <  2:426913/140‑1 (MQ=255)
                                                  tcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgcgg                                                                                       >  1:825396/1‑137 (MQ=255)
                                                  tcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGt                                                                                    <  1:144733/140‑1 (MQ=255)
                                                  tcATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCAGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTgcgg                                                                                       <  2:825396/137‑1 (MQ=255)
                                                             tACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAAt                                                                         >  2:299387/1‑140 (MQ=255)
                                                                  tCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGaa                                                                    >  1:148644/1‑140 (MQ=255)
                                                                    aTTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaag                                                                  <  2:368140/140‑1 (MQ=255)
                                                                    aTTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaag                                                                  >  2:553120/1‑140 (MQ=255)
                                                                       aCTTGTTTCAGGCATGCCCGAGCGGCAAGTGCATAACCCGATCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaag                                                               >  2:676587/1‑140 (MQ=255)
                                                                         ttGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCg                                                             <  2:642223/140‑1 (MQ=255)
                                                                           gTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                                           <  1:208430/140‑1 (MQ=255)
                                                                           gTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                                           <  2:391215/140‑1 (MQ=255)
                                                                           gTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTa                                                           <  1:553120/140‑1 (MQ=255)
                                                                              tCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTg                                                        <  1:160432/140‑1 (MQ=255)
                                                                               cAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGc                                                       <  2:629179/140‑1 (MQ=255)
                                                                                 ggCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCca                                                     <  2:69123/140‑1 (MQ=255)
                                                                                 ggCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCca                                                     <  1:466839/140‑1 (MQ=255)
                                                                                   cATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCcaca                                                   <  2:407379/140‑1 (MQ=255)
                                                                                      gCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaag                                                               <  1:22073/125‑1 (MQ=255)
                                                                                      gCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAaagaag                                                               >  2:22073/1‑125 (MQ=255)
                                                                                      gCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActc                                                >  2:59165/1‑140 (MQ=255)
                                                                                      gCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActc                                                >  1:528121/1‑140 (MQ=255)
                                                                                       cccGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActct                                               >  2:504182/1‑140 (MQ=255)
                                                                                       cccGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActct                                               >  2:389588/1‑140 (MQ=255)
                                                                                       cccGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActct                                               >  2:253495/1‑140 (MQ=255)
                                                                                       cccGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActct                                               >  1:123920/1‑140 (MQ=255)
                                                                                       cccGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACAGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACActct                                               >  2:552181/1‑140 (MQ=255)
                                                                                            gTGGCAAGTGCATAACCCGAGCGACAAACATCT‑CAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGAt                                         >  2:556188/1‑140 (MQ=255)
                                                                                             tGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGAt                                         <  1:299387/140‑1 (MQ=255)
                                                                                                cAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAg                                      >  2:541118/1‑140 (MQ=255)
                                                                                                   gTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCt                                   <  2:770127/140‑1 (MQ=255)
                                                                                                        tAACCCGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTgaga                              <  1:59165/140‑1 (MQ=255)
                                                                                                           cccGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATaa                           >  2:791560/1‑140 (MQ=255)
                                                                                                             cGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATg                         <  2:363763/140‑1 (MQ=255)
                                                                                                             cGAGCGACAAACATCTCCAGAGCTGGCATTGCTGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATg                         <  1:389588/140‑1 (MQ=255)
                                                                                                             cGAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATg                         <  1:323311/140‑1 (MQ=255)
                                                                                                              gAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGc                        >  1:75206/1‑140 (MQ=255)
                                                                                                              gAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGc                        >  1:93695/1‑140 (MQ=255)
                                                                                                              gAGCGACAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGc                        >  1:839249/1‑140 (MQ=255)
                                                                                                                   aCAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTAc                   <  1:541118/140‑1 (MQ=255)
                                                                                                                    cAAACATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTAcc                  >  2:864044/1‑140 (MQ=255)
                                                                                                                    cAAACATCT‑CAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCa                 <  1:556188/140‑1 (MQ=255)
                                                                                                                     aaaCATCTCCAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCa                 >  1:429796/1‑140 (MQ=255)
                                                                                                                             ccAGAGCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATg         <  2:839249/140‑1 (MQ=255)
                                                                                                                                 agCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAAcc     >  2:736322/1‑140 (MQ=255)
                                                                                                                                 agCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAAcc     >  2:848449/1‑140 (MQ=255)
                                                                                                                                  gCTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCg    >  2:538653/1‑140 (MQ=255)
                                                                                                                                   cTGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGc   >  1:229772/1‑140 (MQ=255)
                                                                                                                                    tGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCt  <  2:339947/140‑1 (MQ=255)
                                                                                                                                    tGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCt  <  2:229772/140‑1 (MQ=255)
                                                                                                                                    tGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCt  <  2:123920/140‑1 (MQ=255)
                                                                                                                                    tGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCt  <  1:848449/140‑1 (MQ=255)
                                                                                                                                    tGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCt  <  1:791560/140‑1 (MQ=255)
                                                                                                                                    tGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCt  <  1:538548/140‑1 (MQ=255)
                                                                                                                                    tGGCATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCt  <  2:857437/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TCGCTGCGTAAATCGGTATGCAGCGATCTCCTCACTCTTTTTAATTCTCCTCATTCGGCATTACCGTCATTACTTGTTTCAGGCATGCCCGAGTGGCAAGTGCATAACCCGAGCGACAAACATCTCCAGAGCTGGTATTGCCGTCAGCTGCGTTCTGCACTGTTATTTCATGAGCCACGGATTGCGGCGTTGCAGGTTAATTTGAAAGAAGCGTACTGCCACACTCTGGCGATCAGCCTTGAGATAATGCTGTACCACGACGATGAACCGCT  >  NC_000913/4557050‑4557321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A