Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,558,573	G→T	V318V (GTC→GTA)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,558,573	0	G	T	100.0%	174.4 / NA	49	V318V (GTC→GTA)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (26/23); total (26/23)

CGTCATGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTGACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAACGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCC  >  NC_000913/4558447‑4558708

ggTCATGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACAGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCa                                                                                                                            <  2:895724/139‑1 (MQ=255)

cGTCATGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCa                                                                                                                            <  2:1049064/140‑1 (MQ=255)

cGTCATGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCACCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCa                                                                                                                            <  1:644292/140‑1 (MQ=255)

    aTGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGt                                                                                                                        >  2:721868/1‑140 (MQ=255)

    aTGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGt                                                                                                                        >  2:134701/1‑140 (MQ=255)

    aTGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGt                                                                                                                        >  2:255655/1‑140 (MQ=255)

    aTGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGt                                                                                                                        >  1:456154/1‑140 (MQ=255)

              aGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCaa                                                                                                               <  2:653519/139‑1 (MQ=255)

              aGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCaa                                                                                                               >  1:653519/1‑139 (MQ=255)

              aGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAAc                                                                                                              >  2:128020/1‑140 (MQ=255)

                  aGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGttt                                                                                                          >  2:244523/1‑140 (MQ=255)

                         ttGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAAcc                                                                                                   >  2:1068368/1‑140 (MQ=255)

                         ttGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAAcc                                                                                                   >  1:755215/1‑140 (MQ=255)

                            ccTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGc                                                                                                <  2:344065/140‑1 (MQ=255)

                             cTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGCTTCAAAACCGGCa                                                                                               <  2:1189512/140‑1 (MQ=255)

                              tGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCaa                                                                                              <  1:721868/140‑1 (MQ=255)

                              tGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCaa                                                                                              >  1:1245090/1‑140 (MQ=255)

                                gCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCGTCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAaca                                                                                            <  2:304903/140‑1 (MQ=255)

                                 cAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAacac                                                                                           <  1:947931/140‑1 (MQ=255)

                                     aTTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGat                                                                                       <  1:778522/140‑1 (MQ=255)

                                     aTTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGat                                                                                       <  1:129002/140‑1 (MQ=255)

                                     aTTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTAAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGat                                                                                       <  1:255655/140‑1 (MQ=255)

                                          gCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATggg                                                                                  >  2:788947/1‑140 (MQ=255)

                                             tttCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTa                                                                               <  2:1150070/140‑1 (MQ=255)

                                                cccGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAAt                                                                            >  1:484355/1‑140 (MQ=255)

                                                           tAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCa                                                                 <  1:836669/140‑1 (MQ=255)

                                                                   ccGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCgaagag                                                         <  2:1023081/140‑2 (MQ=255)

                                                                   ccGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCgaagag                                                         >  2:1108772/1‑139 (MQ=255)

                                                                                  gTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGtt                                          <  2:1245090/140‑1 (MQ=255)

                                                                                     aGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGcc                                       >  2:947872/1‑140 (MQ=255)

                                                                                     aGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGcc                                       >  1:115933/1‑140 (MQ=255)

                                                                                     aGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGcc                                       >  1:197264/1‑140 (MQ=255)

                                                                                     aGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGcc                                       >  1:315309/1‑140 (MQ=255)

                                                                                     aGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGcc                                       >  1:207751/1‑140 (MQ=255)

                                                                                       cGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGt                                     <  2:207751/140‑1 (MQ=255)

                                                                                       cGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGt                                     >  2:47749/1‑140 (MQ=255)

                                                                                         ggCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCg                                   <  1:788947/140‑1 (MQ=255)

                                                                                         ggCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCg                                   >  2:544378/1‑140 (MQ=255)

                                                                                            gcAGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAg                                >  2:766116/1‑140 (MQ=255)

                                                                                              aGGGCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCt                              <  1:1068368/140‑1 (MQ=255)

                                                                                               gggCATCGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTg                             >  1:992796/1‑140 (MQ=255)

                                                                                                     cGCTGATACTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTg                       >  2:845383/1‑140 (MQ=255)

                                                                                                             cTGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGc               <  1:150885/140‑1 (MQ=255)

                                                                                                              tGAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGcc              <  1:244523/140‑1 (MQ=255)

                                                                                                               gAAATCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCa             >  2:359389/1‑140 (MQ=255)

                                                                                                                aaaTCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAg            <  2:755215/140‑1 (MQ=255)

                                                                                                                  aTCATAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCg          <  1:947872/140‑1 (MQ=255)

                                                                                                                      tAGTCTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAAt      >  1:338571/1‑140 (MQ=255)

                                                                                                                          cTTTTACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAGCGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATAccc  >  2:1174692/1‑140 (MQ=255)

CGTCATGACCAGCAAGTCAGCATCATTGCCTGGCAGAATTTCGCCTTTCCCGGTCAGGTTAAGGAAACCGGCTACGCTACTGGTGAGCGGGCGCAGGGCATCGCTGATACTGAAATCATAGTCTTTGACCAGCACCTGCACGGTTTCCAGCAACGTTTCAAAACCGGCAACACCGATATGGGTTAAATTCCCTTCGTCATCGAAGAACGGCTGGCTACCGTTGCCGTCGGAGCTGAGGGTGACGCGTGCCAGCGGAATACCC  >  NC_000913/4558447‑4558708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A