Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,563,074	A→G	V177A (GTC→GCC)	yjiK ←	uncharacterized protein YjiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,563,074	0	A	G	100.0%	140.4 / NA	40	V177A (GTC→GCC)	yjiK	uncharacterized protein YjiK
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/13); total (27/13)

AGCACCAGAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGACCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGATTTTTACTTCCGAGTT  >  NC_000913/4562942‑4563210

aGCACCAGAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAAt                                                                                                                                   >  1:219878/1‑140 (MQ=255)

 gCACCAGAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATc                                                                                                                                  >  1:566767/1‑140 (MQ=255)

  cACCAGAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCg                                                                                                                                 >  1:782200/1‑140 (MQ=255)

  cACCAGAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCg                                                                                                                                 >  1:565525/1‑140 (MQ=255)

   aCCAGAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCgg                                                                                                                                >  2:23853/1‑140 (MQ=255)

       gAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGAttt                                                                                                                            >  1:240303/1‑140 (MQ=255)

           cGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTtct                                                                                                                        <  1:801194/140‑1 (MQ=255)

             tATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTctct                                                                                                                      >  1:169103/1‑140 (MQ=255)

                      gCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTCGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAAc                                                                                                             >  1:157521/1‑140 (MQ=255)

                          gTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGa                                                                                                         <  1:226589/140‑1 (MQ=255)

                               aTTCTGCGCCGGACACATCATCTATAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAgtg                                                                                                    <  1:664646/140‑1 (MQ=255)

                               aTTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAgtg                                                                                                    <  1:308422/140‑1 (MQ=255)

                                ttCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTT‑AAAAACCAGAAAGtgtg                                                                                                  <  2:219878/140‑1 (MQ=255)

                                                 catcTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATaa                                                                                  <  1:703964/140‑1 (MQ=255)

                                                        aGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAAcc                                                                           >  1:148663/1‑140 (MQ=255)

                                                         gTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAAccc                                                                          >  1:178585/1‑140 (MQ=255)

                                                           aaaTTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCtt                                                                        <  1:352859/140‑1 (MQ=255)

                                                               tGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCaaa                                                                    >  2:864895/1‑140 (MQ=255)

                                                                    ttGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGc                                                               >  2:519607/1‑140 (MQ=255)

                                                                     tGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCa                                                              <  2:178585/140‑1 (MQ=255)

                                                                          tGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAAttgg                                                         <  2:566767/140‑1 (MQ=255)

                                                                               cGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTggg                                                    <  2:148663/140‑1 (MQ=255)

                                                                               cGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTggg                                                    <  2:782200/140‑1 (MQ=255)

                                                                               cGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTggg                                                    >  2:597189/1‑140 (MQ=255)

                                                                               cGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTggg                                                    >  2:380106/1‑140 (MQ=255)

                                                                               cGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTggg                                                    >  1:792540/1‑140 (MQ=255)

                                                                                 tCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGa                                                  >  2:518516/1‑140 (MQ=255)

                                                                                  cTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATCGGTTGGGGAg                                                 >  2:534985/1‑140 (MQ=255)

                                                                                            gtgtAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGAAGCg                                       >  2:820997/1‑140 (MQ=255)

                                                                                                         cTACTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGatttttatttt                          >  2:108809/1‑140 (MQ=255)

                                                                                                            cTTAACAGCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTAttttttt                       >  2:382479/1‑140 (MQ=255)

                                                                                                                   gCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGAtt                >  1:676744/1‑140 (MQ=255)

                                                                                                                   gCCCGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGAtt                >  2:774471/1‑140 (MQ=255)

                                                                                                                    cccGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGAttt               >  2:109012/1‑140 (MQ=255)

                                                                                                                    cccGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGAttt               >  2:454407/1‑140 (MQ=255)

                                                                                                                      cGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGAttttt             >  1:297323/1‑140 (MQ=255)

                                                                                                                      cGTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGAttttt             >  1:538735/1‑140 (MQ=255)

                                                                                                                       gTTAACTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGATTTTTa            <  1:519607/140‑1 (MQ=255)

                                                                                                                           aCTTTGTAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGATTTTTACTTc        <  1:77553/140‑1 (MQ=255)

                                                                                                                                 tAGGCCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGATTTTTACTTCCGAGtt  >  1:804793/1‑140 (MQ=255)

AGCACCAGAAGCGTATTTTTTTGCTGGTTAAATTCTGCGCCGGACACATCATCTAAAGTAAATTGCCGTTGTAGTGCCTCGTCTTTGCTGATGTGTAATTCATTGCTACTTAACAGCCCGTTAACTTTGTAGACCTCAATCGGATTTTTCTCTTTAAAAAACCAGAAAGTGTGATCCTGGCGAGAATAAGCCAACCCTTCAAAACCGCAATTGGTTGGGGAGTCTTGCAGCGGGATTTTTATTTTTTTGAGGATTTTTACTTCCGAGTT  >  NC_000913/4562942‑4563210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A