Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,526	C→T	D109N (GAC→AAC)	yjiM ←	putative dehydratase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,526	0	C	T	100.0%	209.6 / NA	58	D109N (GAC→AAC)	yjiM	putative dehydratase subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (39/19); total (39/19)

TCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATGGTTTC  >  NC_000913/4565390‑4565661

tctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCa                                                                                                                                      <  1:983768/140‑1 (MQ=255)

    ccGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAggtg                                                                                                                                  >  1:468076/1‑140 (MQ=255)

       ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCa                                                                                                                                      >  2:977773/1‑133 (MQ=255)

       ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCa                                                                                                                                      <  1:977773/133‑1 (MQ=255)

       ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGtt                                                                                                                               >  1:249830/1‑140 (MQ=255)

           ttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCAc                                                                                                                           <  1:1015563/140‑1 (MQ=255)

             gCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCg                                                                                                                         <  2:708227/140‑1 (MQ=255)

             gCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCg                                                                                                                         <  2:468076/140‑1 (MQ=255)

                  cgcAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGaccac                                                                                                                    <  1:138998/140‑1 (MQ=255)

                          ctcGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCg                                                                                                            <  2:249830/140‑1 (MQ=255)

                                    cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAaagtagaa                                                                                                  >  1:603788/1‑140 (MQ=255)

                                        acgcacgcGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAaagtagaagtag                                                                                              >  1:720091/1‑140 (MQ=255)

                                           cgcgcgAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAgggg                                                                                           >  1:768550/3‑140 (MQ=255)

                                           cacgcGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAgggg                                                                                           >  1:683673/1‑140 (MQ=255)

                                              gcgAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCAt                                                                                        >  2:637369/1‑140 (MQ=255)

                                                gAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCAttt                                                                                      <  2:683673/140‑1 (MQ=255)

                                                gAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCAttt                                                                                      <  2:823522/140‑1 (MQ=255)

                                                        gTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGttt                                                                              >  1:286205/1‑140 (MQ=255)

                                                                    gTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGt                                                                  >  2:531139/1‑140 (MQ=255)

                                                                    gTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGt                                                                  >  1:856377/1‑140 (MQ=255)

                                                                     ttGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTa                                                                 >  1:578839/1‑140 (MQ=255)

                                                                      tGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAg                                                                <  1:956935/140‑1 (MQ=255)

                                                                             ttGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCttt                                                         >  2:833613/1‑140 (MQ=255)

                                                                                  tCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATc                                                    >  1:130950/1‑140 (MQ=255)

                                                                                    acaTGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAg                                                  <  2:653700/140‑1 (MQ=255)

                                                                                    acaTGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAg                                                  <  1:531139/140‑1 (MQ=255)

                                                                                         aaCAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGc                                             >  2:348306/1‑140 (MQ=255)

                                                                                          aCAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCa                                                   >  1:638726/1‑133 (MQ=255)

                                                                                          aCAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCa                                                   <  2:638726/133‑1 (MQ=255)

                                                                                            aGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGTGGGCaga                                          <  1:273292/140‑1 (MQ=255)

                                                                                             ggCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCagag                                         >  1:252164/1‑140 (MQ=255)

                                                                                               cTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGt                                       >  1:338501/1‑140 (MQ=255)

                                                                                                ttAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGtt                                      >  1:777032/1‑140 (MQ=255)

                                                                                                  aaaCTCCGCCATGTATTCATACATTTTCTTTTGGCCGTTGCAGGTGGTTTCGCCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTgc                                    <  2:696990/140‑1 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGcgcg                                 >  1:180626/1‑137 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGcgcg                                 <  2:180626/137‑1 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  2:744904/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  2:786666/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  1:225149/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  2:628767/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  1:624632/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  2:105351/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  2:1003730/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  1:55614/1‑140 (MQ=255)

                                                                                                        cGCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCa                              >  1:543734/1‑140 (MQ=255)

                                                                                                         gCCATGTATTCATACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAg                             >  1:901218/1‑140 (MQ=255)

                                                                                                                    aTACATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCg                  >  2:927933/1‑140 (MQ=255)

                                                                                                                      aCATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGct                >  2:367756/1‑140 (MQ=255)

                                                                                                                       cATTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGctt               >  2:575394/1‑140 (MQ=255)

                                                                                                                        aTTTTCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGcttc              >  2:915532/1‑140 (MQ=255)

                                                                                                                          tttCTTTTTGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGcttctt            >  1:374697/1‑140 (MQ=255)

                                                                                                                              tttttGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAAt        <  1:161586/140‑1 (MQ=255)

                                                                                                                               ttttGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATg       >  2:777446/1‑140 (MQ=255)

                                                                                                                                 ttGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATGGt     <  1:915532/140‑1 (MQ=255)

                                                                                                                                  tGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATGGtt    >  1:437580/1‑140 (MQ=255)

                                                                                                                                  tGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATGGt     >  2:128854/1‑139 (MQ=255)

                                                                                                                                  tGCCGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATGGt     <  1:128854/139‑1 (MQ=255)

                                                                                                                                    ccGTTGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATGGTTtc  >  2:243316/1‑140 (MQ=255)

TCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGCCGAAGCCGTAGCTGCTTTTAATCAGCGGGCAGAGGTTGCGCGGCAGATCTTTCTCCGCTTCTTCAATGGTTTC  >  NC_000913/4565390‑4565661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A