Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,580,860	(C)_6→7	coding (603/1395 nt)	hsdS ←	type I restriction enzyme EcoKI specificity protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,580,854	1	.	C	100.0%	168.6 / NA	50	G203G (GGC→GGG)	hsdS	type I restriction enzyme EcoKI specificity protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (21/29); total (21/29)

GACCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑CCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAGTGGTGGGA  >  NC_000913/4580715‑4580987

gACCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg                                                                                                                                        >  2:558929/1‑140 (MQ=255)

 aCCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg‑c                                                                                                                                      <  2:882138/140‑1 (MQ=255)

 aCCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg‑c                                                                                                                                      <  2:761870/140‑1 (MQ=255)

 aCCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg‑c                                                                                                                                      <  2:263001/140‑1 (MQ=255)

  ccAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg‑c                                                                                                                                      >  2:99666/1‑139 (MQ=255)

  ccAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg‑c                                                                                                                                      <  1:99666/139‑1 (MQ=255)

  ccAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑cc                                                                                                                                     >  1:645830/1‑140 (MQ=255)

  ccAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑cc                                                                                                                                     >  2:253624/1‑140 (MQ=255)

  ccAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑cc                                                                                                                                     >  2:288297/1‑140 (MQ=255)

  ccAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑cc                                                                                                                                     >  2:376208/1‑140 (MQ=255)

       accacTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg‑c                                                                                                                                      >  1:414127/1‑134 (MQ=255)

       accacTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTgcg‑c                                                                                                                                      <  2:414127/134‑1 (MQ=255)

            tttCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATAc                                                                                                                            >  2:145375/1‑140 (MQ=255)

            tttCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑ccc                                                                                                                                    >  1:305620/1‑131 (MQ=255)

            tttCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑ccc                                                                                                                                    <  2:305620/131‑1 (MQ=255)

              tCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCg                                                                                                                          >  1:147929/1‑140 (MQ=255)

                         tgatgaAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGaaaa                                                                                                               <  1:542386/140‑1 (MQ=255)

                                aGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCa                                                                                                        <  2:645830/140‑1 (MQ=255)

                                 gACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAg                                                                                                       >  1:298751/1‑140 (MQ=255)

                                   ccATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGa                                                                                                     <  1:145375/140‑1 (MQ=255)

                                        aCGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTtgt                                                                                                <  2:287851/140‑1 (MQ=255)

                                                 aGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGc                                                                                       >  1:60253/1‑140 (MQ=255)

                                                 aGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTATGGGATTTGc                                                                                       >  2:590259/1‑140 (MQ=255)

                                                   ttAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTc                                                                                     >  2:248447/1‑140 (MQ=255)

                                                        atagatTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAAc                                                                                >  1:317965/1‑140 (MQ=255)

                                                           gatTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTg                                                                             >  1:211247/1‑140 (MQ=255)

                                                           gatTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTg                                                                             <  1:675525/140‑1 (MQ=255)

                                                            atTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCt                                                                                      <  2:561143/130‑1 (MQ=255)

                                                            atTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCt                                                                                      >  1:561143/1‑130 (MQ=255)

                                                            atTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGc                                                                            >  1:262383/1‑140 (MQ=255)

                                                             ttCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCt                                                                           >  1:781318/1‑140 (MQ=255)

                                                              tCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCtt                                                                          <  2:317965/140‑1 (MQ=255)

                                                              tCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCtt                                                                          <  2:60253/140‑1 (MQ=255)

                                                                 aaaTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTgg                                                                       <  2:789810/140‑1 (MQ=255)

                                                                 aaaTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTgg                                                                       <  1:590259/140‑1 (MQ=255)

                                                                  aaTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGt                                                                      <  2:500538/140‑1 (MQ=255)

                                                                  aaTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGt                                                                      <  2:298751/140‑1 (MQ=255)

                                                                   aTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTg                                                                     <  1:376208/140‑1 (MQ=255)

                                                                   aTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTg                                                                     <  2:432193/140‑1 (MQ=255)

                                                                   aTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTg                                                                     <  1:373805/140‑1 (MQ=255)

                                                                   aTTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTg                                                                     <  1:248447/140‑1 (MQ=255)

                                                                      tAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTgttg                                                                  <  2:262383/140‑4 (MQ=255)

                                                                       aaCTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGt                                                                 >  2:526316/1‑140 (MQ=255)

                                                                                       aaTGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCcagcag                                                 >  1:837786/1‑140 (MQ=255)

                                                                                         tGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCg                                               >  2:453547/1‑140 (MQ=255)

                                                                                            tGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTAcc                                                            >  1:76707/1‑124 (MQ=255)

                                                                                            tGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTAcc                                                            <  2:76707/124‑1 (MQ=255)

                                                                                            tGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTAt                                            >  1:759124/1‑140 (MQ=255)

                                                                                              cGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCg                                          >  1:623048/1‑140 (MQ=255)

                                                                                                gCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAg                                        <  2:623048/140‑1 (MQ=255)

                                                                                                   cAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGttt                                     >  2:657988/1‑140 (MQ=255)

                                                                                                          aCGCCATTTTTCTGTCAATTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCg                              <  1:453547/140‑1 (MQ=255)

                                                                                                                          aaTTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCgg              <  2:759124/140‑1 (MQ=255)

                                                                                                                           aTTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGc             <  1:288297/140‑1 (MQ=255)

                                                                                                                           aTTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGc             <  1:657988/140‑1 (MQ=255)

                                                                                                                           aTTTTCCATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGc             <  2:147929/140‑1 (MQ=255)

                                                                                                                                 cATTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAgtgg       <  1:558929/140‑1 (MQ=255)

                                                                                                                                  aTTAGCTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAgtggt      <  2:270327/140‑1 (MQ=255)

                                                                                                                                  aTTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCaa           >  2:739148/1‑135 (MQ=255)

                                                                                                                                  aTTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCaa           <  1:739148/135‑1 (MQ=255)

                                                                                                                                  aTTAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAgtggt      <  2:625560/140‑1 (MQ=255)

                                                                                                                                    tAACTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAGtggtgg    >  1:104407/1‑140 (MQ=255)

                                                                                                                                      aCTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAGTGGTGGGa  <  1:253624/140‑1 (MQ=255)

                                                                                                                                      aCTGCGCCCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAGTGGTGGGa  >  2:491965/1‑140 (MQ=255)

GACCAACACCACTTTCATTTGGCTTTGATGAAAGACCATTACGTAATTCAGTTAAGATAGATTCAAAATTTAACTTCTTAAATACAGAATGTTGCGGCTCAAAATTACGCCATTTTTCTGTCAATTTTCCATTAACTGCG‑CCCCCCAATACCGCTTGACGAAAACGTTTCAGGATTTGTGGGATTTGCTCAAAACGTGCTTTGGTGCTGTCTACCTGCGCCAGCAGCGTATCGAGTTTTTCAGCGATGATTTTTTGTTCGGCAAGTGGTGGGA  >  NC_000913/4580715‑4580987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A