Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,371	T→C	L154L (TTG→CTG)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,371	0	T	C	100.0%	139.9 / NA	40	L154L (TTG→CTG)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/23); total (17/23)

CCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCG  >  NC_000913/4626237‑4626504

ccACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCg                                                                                                                                  <  2:792660/140‑1 (MQ=255)

      tgctACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAAcc                                                                                                                            <  2:837369/140‑1 (MQ=255)

          aCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCa                                                                                                                        <  2:229874/140‑1 (MQ=255)

          aCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCa                                                                                                                        <  1:779/140‑1 (MQ=255)

          aCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCa                                                                                                                        <  1:585704/140‑1 (MQ=255)

               aaaCGCTGTGCAAACTAGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGaa                                                                                                                   <  1:387244/140‑1 (MQ=255)

                aaCGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAAc                                                                                                                  >  1:402350/1‑140 (MQ=255)

                  cGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAg                                                                                                                >  1:689803/1‑140 (MQ=255)

                      gtgCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCt                                                                                                            >  2:57353/1‑140 (MQ=255)

                        gCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGc                                                                                                          >  1:754387/1‑140 (MQ=255)

                             cTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGAt                                                                                                     <  2:190913/140‑1 (MQ=255)

                               ggCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTg                                                                                                   <  1:118366/140‑1 (MQ=255)

                               ggCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTg                                                                                                   >  2:403456/1‑140 (MQ=255)

                                   cagcagATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCga                                                                                               <  2:149090/140‑1 (MQ=255)

                                        gATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGaagaag                                                                                          >  1:421085/1‑140 (MQ=255)

                                                cGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCg                                                                                  <  1:57353/140‑1 (MQ=255)

                                                   tGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAg                                                                               >  1:559446/1‑140 (MQ=255)

                                                   tGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAg                                                                               <  1:173930/140‑1 (MQ=255)

                                                      aTGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTg                                                                            >  2:76730/1‑140 (MQ=255)

                                                       tGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGa                                                                           <  1:76730/140‑1 (MQ=255)

                                                             cGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTaa                                                                     >  1:286156/1‑140 (MQ=255)

                                                                  aaGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGAc                                                                >  2:734354/1‑140 (MQ=255)

                                                                   agagTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACt                                                               <  1:688260/140‑1 (MQ=255)

                                                                   agagTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACt                                                               <  1:23302/140‑1 (MQ=255)

                                                                        cGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATc                                                          <  1:403456/140‑1 (MQ=255)

                                                                         gCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATcc                                                         <  2:738252/140‑1 (MQ=255)

                                                                              aaCAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTg                                                    <  2:421085/140‑1 (MQ=255)

                                                                                   gTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCa                                               <  2:689803/140‑1 (MQ=255)

                                                                                         aTGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGc                                         <  1:807357/140‑1 (MQ=255)

                                                                                            cgcgcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGa                                      >  2:850527/1‑140 (MQ=255)

                                                                                            cgcgcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGa                                      >  1:165154/1‑140 (MQ=255)

                                                                                            cgcgcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGAGCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGa                                      >  2:811695/1‑140 (MQ=255)

                                                                                              cgcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATg                                    >  2:615981/1‑140 (MQ=255)

                                                                                                  cATCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACa                                >  1:717855/1‑140 (MQ=255)

                                                                                                   aTCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAg                               >  2:744733/1‑140 (MQ=255)

                                                                                                    tCGCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGt                              <  1:615981/140‑1 (MQ=255)

                                                                                                      gCCTTGGCCTGCCGACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCa                            <  2:286156/140‑1 (MQ=255)

                                                                                                                   gACTGACAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCg               <  2:754387/140‑1 (MQ=255)

                                                                                                                         cAATCTCAATATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGgcgc         <  2:402350/140‑1 (MQ=255)

                                                                                                                                aaTATGCTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCg  >  1:158442/1‑140 (MQ=255)

CCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGCTGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCG  >  NC_000913/4626237‑4626504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A