Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	180,910	T→C	S9S (AGT→AGC)	degP →	periplasmic serine endoprotease DegP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	180,910	0	T	C	100.0%	166.1 / NA	46	S9S (AGT→AGC)	degP	periplasmic serine endoprotease DegP
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/22); total (24/22)

CAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTGATGCCTTCAGTGGT  >  NC_000913/180776‑181041

cAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACt                                                                                                                                >  1:141248/1‑140 (MQ=255)

    aaaTTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGct                                                                                                                            <  1:1105696/140‑1 (MQ=255)

    aaaTTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGct                                                                                                                            <  2:141248/140‑1 (MQ=255)

    aaaTTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGct                                                                                                                            <  1:250022/140‑1 (MQ=255)

           tACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGtt                                                                                                                     <  2:1017928/140‑1 (MQ=255)

                     aGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGc                                                                                                           <  2:505305/140‑1 (MQ=255)

                       aaaCTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGt                                                                                                         <  1:1030549/140‑1 (MQ=255)

                       aaaCTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGt                                                                                                         <  1:517244/140‑1 (MQ=255)

                       aaaCTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGt                                                                                                         >  2:471178/1‑140 (MQ=255)

                               gTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCg                                                                                                 <  2:1247830/140‑1 (MQ=255)

                                   ggAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGt                                                                                                         <  2:568951/128‑1 (MQ=255)

                                   ggAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGt                                                                                                         >  1:568951/1‑128 (MQ=255)

                                    gAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGctctc                                                                                            <  2:934631/140‑1 (MQ=255)

                                     aaCTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGTGTTATCTCCGctctct                                                                                           >  2:758546/1‑140 (MQ=255)

                                     aaCTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGctctct                                                                                           >  1:288883/1‑140 (MQ=255)

                                     aaCTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGctctct                                                                                           >  2:161268/1‑140 (MQ=255)

                                          cAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAAc                                                                                      >  1:1086024/1‑140 (MQ=255)

                                           aGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAAcg                                                                                     <  2:288883/140‑1 (MQ=255)

                                                  aaaaCGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTg                                                                              >  2:1023978/1‑140 (MQ=255)

                                                         aTCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGActtc                                                                       >  2:711587/1‑140 (MQ=255)

                                                                   cacaGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGActtct                                                                      <  1:1245653/131‑1 (MQ=255)

                                                                   cacaGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGActtct                                                                      >  2:1245653/1‑131 (MQ=255)

                                                                   cacaGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACg                                                             >  2:1072535/1‑140 (MQ=255)

                                                                        cAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGc                                                        >  2:628646/1‑140 (MQ=255)

                                                                                cGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGa                                                >  1:1104637/1‑140 (MQ=255)

                                                                                cGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGa                                                >  1:480269/1‑140 (MQ=255)

                                                                                    aTCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGcc                                            <  1:628646/140‑1 (MQ=255)

                                                                                    aTCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGcc                                            <  2:909884/140‑1 (MQ=255)

                                                                                    aTCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGcc                                            <  2:1040756/140‑1 (MQ=255)

                                                                                       tGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAg                                         <  1:161268/140‑1 (MQ=255)

                                                                                         ttAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGcc                                       <  2:1104637/140‑1 (MQ=255)

                                                                                                 gaCTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCg                               >  2:839311/1‑140 (MQ=255)

                                                                                                  aCTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGa                              >  2:1024372/1‑140 (MQ=255)

                                                                                                   cTGAAATACATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGAt                             >  1:265578/1‑140 (MQ=255)

                                                                                                          aCATGAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGaa                      <  2:293043/140‑1 (MQ=255)

                                                                                                              gAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAgg                  >  1:515694/1‑140 (MQ=255)

                                                                                                              gAAAAAAACCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAgg                  >  1:1117304/1‑140 (MQ=255)

                                                                                                                aaaaaaCCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGTGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTg                <  1:758546/140‑1 (MQ=255)

                                                                                                                aaaaaaCCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCAcc                                <  1:578464/124‑1 (MQ=255)

                                                                                                                aaaaaaCCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCAcc                                >  2:578464/1‑124 (MQ=255)

                                                                                                                aaaaaaCCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCAc                                 >  1:812650/1‑123 (MQ=255)

                                                                                                                aaaaaaCCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCAc                                 <  2:812650/123‑1 (MQ=255)

                                                                                                                aaaaaaCCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTg                >  1:435506/1‑140 (MQ=255)

                                                                                                                     aCCACATTAGCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTGATGcc           >  2:613188/1‑140 (MQ=255)

                                                                                                                     aCCACATTAGCACTGAGCACACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTGATGcc           >  1:378528/1‑140 (MQ=255)

                                                                                                                              gCACTGAGCGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTGATGCCTTCAGTGGt  <  1:839311/140‑1 (MQ=255)

CAATAAATTTTTACCTTTTGCAGAAACTTTAGTTCGGAACTTCAGGCTATAAAACGAATCTGAAGAACACAGCAATTTTGCGTTATCTGTTAATCGAGACTGAAATACATGAAAAAAACCACATTAGCACTGAGTGCACTGGCTCTGAGTTTAGGTTTGGCGTTATCTCCGCTCTCTGCAACGGCGGCTGAGACTTCTTCAGCAACGACAGCCCAGCAGATGCCAAGCCTTGCACCGATGCTCGAAAAGGTGATGCCTTCAGTGGT  >  NC_000913/180776‑181041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A