Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	185,773	A→G	L59L (TTG→CTG)	dapD ←	tetrahydrodipicolinate succinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	185,773	0	A	G	100.0%	205.0 / NA	57	L59L (TTG→CTG)	dapD	tetrahydrodipicolinate succinylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/26); total (31/26)

GCGGAAGCCTTCTTTCTGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAACCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGATCTCGGCACG  >  NC_000913/185636‑185905

gcgGAAGCCTTCTTTCTGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGcc                                                                                                                                    >  1:482321/1‑140 (MQ=255)

  ggAAGCCTTCTTTCTGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCAc                                                                                                                                  >  1:767325/1‑140 (MQ=255)

        cttcttTCTGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGc                                                                                                                            <  1:1165227/140‑1 (MQ=255)

          tctttctGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGt                                                                                                                          <  1:792945/140‑1 (MQ=255)

          tctttctGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGt                                                                                                                          <  1:649607/140‑1 (MQ=255)

           ctttctGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTc                                                                                                                         >  2:849531/1‑140 (MQ=255)

                       gTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGTACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCg                                                                                                             >  2:1048479/1‑140 (MQ=255)

                                 gtAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAAtttttt                                                                                                   <  2:149916/140‑1 (MQ=255)

                                  tAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTc                                                                                                  <  2:767325/140‑1 (MQ=255)

                                             tttCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCa                                                                                       <  2:482321/140‑1 (MQ=255)

                                             tttCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCa                                                                                       <  1:265695/140‑1 (MQ=255)

                                             tttCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCa                                                                                       <  1:548601/140‑1 (MQ=255)

                                                   cGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGTACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTgcgc                                                                                 <  1:1048479/140‑1 (MQ=255)

                                                      cACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACg                                                                                         <  1:590000/129‑1 (MQ=255)

                                                      cACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACg                                                                                         >  2:590000/1‑129 (MQ=255)

                                                              cGAAGTATCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCcagc                                                                      <  2:101828/140‑1 (MQ=255)

                                                                aaGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCcagcag                                                                    >  2:1003530/1‑140 (MQ=255)

                                                                aaGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCcagcag                                                                    <  2:909903/140‑1 (MQ=255)

                                                                   tAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGc                                                                 <  1:516919/140‑1 (MQ=255)

                                                                           ttCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCt                                                         >  2:276048/1‑140 (MQ=255)

                                                                                     tCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGc                                               >  1:496413/1‑140 (MQ=255)

                                                                                     tCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGc                                               >  1:489385/1‑140 (MQ=255)

                                                                                     tCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGc                                               >  2:1172296/1‑140 (MQ=255)

                                                                                     tCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGc                                               >  2:1099600/1‑140 (MQ=255)

                                                                                       gATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCtt                                             <  2:496413/140‑1 (MQ=255)

                                                                                        aTCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTc                                            >  1:112857/1‑140 (MQ=255)

                                                                                          cACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTcgc                                          >  2:379970/1‑140 (MQ=255)

                                                                                            ccTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCgg                                        >  1:1233528/1‑140 (MQ=255)

                                                                                                aTTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTaa                                    >  1:472852/1‑140 (MQ=255)

                                                                                                 ttATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAAc                                   <  1:738076/140‑1 (MQ=255)

                                                                                                  tATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACg                                  <  1:1172296/140‑1 (MQ=255)

                                                                                                    tCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGgt                                <  1:379970/140‑1 (MQ=255)

                                                                                                       ttAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCTCTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTc                             >  1:651122/1‑140 (MQ=255)

                                                                                                       ttAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTGCCGCTTCGCGGGTAACGGTGTc                             >  1:518323/1‑140 (MQ=255)

                                                                                                       ttAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTaa                                    >  2:1017106/1‑133 (MQ=255)

                                                                                                       ttAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTaa                                    <  1:1017106/133‑1 (MQ=255)

                                                                                                       ttAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTc                             >  2:574948/1‑140 (MQ=255)

                                                                                                       ttAATACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTc                             >  2:227818/1‑140 (MQ=255)

                                                                                                          aTACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGc                          >  2:148401/1‑140 (MQ=255)

                                                                                                          aTACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGc                          <  2:203454/140‑1 (MQ=255)

                                                                                                           tACGGAAAGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCa                         >  1:1151259/1‑140 (MQ=255)

                                                                                                                aaaGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATtggc                    <  1:849531/140‑1 (MQ=255)

                                                                                                                aaaGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATtggc                    >  2:992478/1‑140 (MQ=255)

                                                                                                                aaaGAGAGCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATtggc                    >  1:1101371/1‑140 (MQ=255)

                                                                                                                    agagCAGCACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTGCCGCTTCGCGGGTAACGGTGTCTGCATtggctggc                <  2:518323/140‑1 (MQ=255)

                                                                                                                      agcagcACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCAtt                       <  2:35589/131‑1 (MQ=255)

                                                                                                                      agcagcACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCAtt                       >  1:35589/1‑131 (MQ=255)

                                                                                                                      agcagcACCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGt              >  1:67335/1‑140 (MQ=255)

                                                                                                                       gcagcaCCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATtggctggc                <  2:1233226/137‑1 (MQ=255)

                                                                                                                       gcagcaCCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATtggctggc                >  1:1233226/1‑137 (MQ=255)

                                                                                                                        cagcaCCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGATAACGGTGTCTGCATTGGCTGGCGTGa            >  1:521807/1‑140 (MQ=255)

                                                                                                                            aCCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGAtctc        >  1:771177/1‑140 (MQ=255)

                                                                                                                            aCCGCTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGAtctc        >  1:39723/1‑140 (MQ=255)

                                                                                                                                cTTTTTTCAGCCACTGATGCGTCACCCTCTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGATCTCGGCa    <  2:651122/140‑1 (MQ=255)

                                                                                                                                cTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTGCCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGATCTCGGCa    >  2:314159/1‑140 (MQ=255)

                                                                                                                                cTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGATCTCGGCa    >  2:462892/1‑140 (MQ=255)

                                                                                                                                cTTTTTTCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGATCTCGGCa    <  1:455320/140‑1 (MQ=255)

                                                                                                                                  tttttCAGCCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGATCTCGGCACg  <  1:875917/140‑1 (MQ=255)

GCGGAAGCCTTCTTTCTGGAAACGTGCTTCGTCGTAGTCGGCGAATTTCATCGGCACTTTGTCGAAGTAGCGGCTTTCTGCCCCTTCGATCACCTGATTATCATTAATACGGAAAGAGAGCAGCACCGCTTTTTTCAACCACTGATGCGTCACCCACTGACCGTCAATTTTTTCCGCTACACGCAGTGCGCCGGAATCCAGCAGGGCGATCACCTGATTTACCGCTTCGCGGGTAACGGTGTCTGCATTGGCTGGCGTGATCTCGGCACG  >  NC_000913/185636‑185905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A