Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 161 75.5 2581324 99.3% 2563254 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,456,980 A→G D184G (GAC→GGC)  paaE → phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,456,9800AG100.0% 184.2 / NA 51D184G (GAC→GGC) paaEphenylacetyl‑CoA 1,2‑epoxidase, reductase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/24);  total (27/24)

CACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCT  >  NC_000913/1456858‑1457114
                                                                                                                          |                                                                                                                                      
cACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACg                                                                                                                       <  2:290050/140‑1 (MQ=255)
          tACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGa                                                                                                             >  2:48761/1‑140 (MQ=255)
            cGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACg                                                                                                           >  1:253418/1‑140 (MQ=255)
             ggTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACgg                                                                                                          >  2:907360/1‑140 (MQ=255)
                 aCCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGTAGCGATCTGCTTCACGGGCGTATTGACGGTGaa                                                                                                      >  1:1029674/1‑140 (MQ=255)
                   cGTACCAGCCAGGGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGaaaa                                                                                                    <  2:39809/140‑1 (MQ=255)
                         agccagAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACa                                                                                              <  1:1105349/140‑1 (MQ=255)
                          gccagAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGTAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAg                                                                                             <  2:1029674/140‑1 (MQ=255)
                           ccagAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGt                                                                                            <  2:178356/140‑1 (MQ=255)
                               agCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACt                                                                                        >  2:175585/1‑140 (MQ=255)
                               agCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACt                                                                                        >  2:12669/1‑140 (MQ=255)
                                    gatgTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACt                                                                                        >  1:78434/1‑135 (MQ=255)
                                    gatgTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACt                                                                                        <  2:78434/135‑1 (MQ=255)
                                    gatgTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTgggg                                                                                   >  2:418454/1‑140 (MQ=255)
                                    gatgTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTgggg                                                                                   >  1:1164941/1‑140 (MQ=255)
                                    gatgTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTgggg                                                                                   >  2:1250035/1‑140 (MQ=255)
                                         ttcaccAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCg                                                                              >  1:896658/5‑140 (MQ=255)
                                         ttCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCg                                                                              >  1:1136593/1‑140 (MQ=255)
                                         ttCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCg                                                                              >  2:54432/1‑140 (MQ=255)
                                          tCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGc                                                                             >  2:605342/1‑140 (MQ=255)
                                            gCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTc                                                                           <  2:1136593/140‑1 (MQ=255)
                                                ggCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTa                                                                       >  1:888415/1‑140 (MQ=255)
                                                   aCTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAAtt                                                                    <  1:605342/140‑1 (MQ=255)
                                                    cTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAAttt                                                                   <  1:162212/140‑1 (MQ=255)
                                                           aCCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCtt                                                            >  1:19539/1‑140 (MQ=255)
                                                            ccTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCttt                                                           >  1:1097824/1‑140 (MQ=255)
                                                             cTGAAAGACAGATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTa                                                          >  1:1077480/1‑140 (MQ=255)
                                                             cTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTa                                                          <  1:203444/140‑1 (MQ=255)
                                                                aaaGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTat                                                         <  2:161137/138‑1 (MQ=255)
                                                                aaaGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTat                                                         >  1:161137/1‑138 (MQ=255)
                                                                 aaGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTatgat                                                      <  2:413900/140‑1 (MQ=255)
                                                                  aGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTatgatg                                                     <  1:12669/140‑1 (MQ=255)
                                                                   gACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTAtgatga                                                    <  2:948703/140‑1 (MQ=255)
                                                                    aCAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAg                                                   <  2:253418/140‑1 (MQ=255)
                                                                    aCAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAg                                                   <  1:418454/140‑1 (MQ=255)
                                                                     cAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAgg                                                  >  2:1200008/1‑140 (MQ=255)
                                                                         tatCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCatt                                              <  1:1250035/140‑1 (MQ=255)
                                                                              tCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAACCCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTT‑GGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTtg                                        <  2:1243993/140‑1 (MQ=255)
                                                                              tCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCatt                                              <  2:119532/135‑1 (MQ=255)
                                                                              tCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCatt                                              >  1:119532/1‑135 (MQ=255)
                                                                                      tACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCcg                                 >  1:367430/1‑140 (MQ=255)
                                                                                       aCAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCcgg                                >  2:949413/1‑140 (MQ=255)
                                                                                          gttgttGTGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCcggcgg                             <  2:795930/140‑1 (MQ=255)
                                                                                            tgttgtGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGgcggcg                           <  1:949413/140‑1 (MQ=255)
                                                                                            tgttgtGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGgcggcg                           <  2:841054/140‑1 (MQ=255)
                                                                                              ttgtGCATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCGCTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCgat                         >  1:12191/1‑140 (MQ=255)
                                                                                                   cATTTTCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATgatgg                    >  2:268674/1‑140 (MQ=255)
                                                                                                     ttttCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATgatggat                  >  2:611932/1‑140 (MQ=255)
                                                                                                     ttttCAGTCAGGAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATgatggat                  >  1:1000040/1‑140 (MQ=255)
                                                                                                               ggAAACCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGaaa        <  1:268674/140‑1 (MQ=255)
                                                                                                                 aaaCCCTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAAcc      <  1:444023/140‑1 (MQ=255)
                                                                                                                   aCCCTCGGCAGCGATCTGCTTCACGGGCGTATTGATGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGc    <  2:888415/140‑1 (MQ=255)
                                                                                                                     ccTCGGCAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCt  <  2:1097824/140‑1 (MQ=255)
                                                                                                                          |                                                                                                                                      
CACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTCAGGAAACCCTCGACAGCGATCTGCTTCACGGGCGTATTGACGGTGAAAAATTACAGTCACTTGGGGCCTCGCTCATTAATTTTCGTCTTTATGATGAGGCATTTATTTGTGGTCCGGCGGCGATGATGGATGACGCGGAAACCGCCT  >  NC_000913/1456858‑1457114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A