Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 161 75.5 2581324 99.3% 2563254 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,536,408 G→A G70G (GGC→GGT)  narV ← nitrate reductase Z subunit gamma

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,536,4080GA98.2% 192.1 / ‑4.2 57G70G (GGC→GGT) narVnitrate reductase Z subunit gamma
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (22/34);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

TCAGCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATGCCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAG  >  NC_000913/1536275‑1536543
                                                                                                                                     |                                                                                                                                       
tCAGCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAAc                                                                                                                                   <  1:479960/140‑1 (MQ=255)
  aGCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATGCCGAACaa                                                                                                                                 <  1:54170/140‑1 (MQ=255)
   gCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAg                                                                                                                                >  2:118609/1‑140 (MQ=255)
     cTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTg                                                                                                                              >  1:1074226/1‑140 (MQ=255)
       gcgcCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGcc                                                                                                                            <  2:104677/140‑1 (MQ=255)
       gcgcCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGcc                                                                                                                            >  1:81446/1‑140 (MQ=255)
                  gTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATc                                                                                                                 >  2:758837/1‑140 (MQ=255)
                     cTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATcccc                                                                                                              >  2:490134/1‑140 (MQ=255)
                     cTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATcccc                                                                                                              >  1:900601/1‑140 (MQ=255)
                        cgccgcCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCaaa                                                                                                           >  1:1115451/1‑140 (MQ=255)
                          ccgccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAAt                                                                                                         <  2:451932/140‑1 (MQ=255)
                            gccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGc                                                                                                       <  1:1021259/140‑1 (MQ=255)
                            gccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGc                                                                                                       <  2:900601/140‑1 (MQ=255)
                            gccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGc                                                                                                       <  2:834790/140‑1 (MQ=255)
                            gccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGc                                                                                                       <  2:379181/140‑1 (MQ=255)
                            gccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGc                                                                                                       <  1:490134/140‑1 (MQ=255)
                            gccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGc                                                                                                       <  1:133057/140‑1 (MQ=255)
                             ccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGcc                                                                                                      >  2:643978/1‑140 (MQ=255)
                             ccAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGcc                                                                                                      >  1:1255649/1‑140 (MQ=255)
                                 tCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGata                                                                                                  <  2:621562/140‑1 (MQ=255)
                                      gTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGaa                                                                                             <  2:309991/140‑1 (MQ=255)
                                           cacacCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGAt                                                                                        <  2:1074226/140‑1 (MQ=255)
                                             cacCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTc                                                                                      <  2:412705/140‑1 (MQ=255)
                                              acCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCg                                                                                     <  2:1255649/140‑1 (MQ=255)
                                                cGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGAc                                                                                   <  1:118609/140‑1 (MQ=255)
                                                cGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGAc                                                                                   <  1:1162072/140‑1 (MQ=255)
                                                cGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGAc                                                                                   >  1:623623/1‑140 (MQ=255)
                                                cGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGAc                                                                                   >  1:1049312/1‑140 (MQ=255)
                                                cGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGAc                                                                                   <  1:758837/140‑1 (MQ=255)
                                                 gCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGAcc                                                                                  >  1:303236/1‑140 (MQ=255)
                                                  cAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCg                                                                                     <  1:748138/136‑1 (MQ=255)
                                                  cAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCg                                                                                     >  2:748138/1‑136 (MQ=255)
                                                  cAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCa                                                                                 <  2:243317/140‑1 (MQ=255)
                                                    aaTACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCata                                                                               <  1:1222316/140‑1 (MQ=255)
                                                       aCCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCa                                                                            <  2:1049312/140‑1 (MQ=255)
                                                       aCCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCa                                                                            <  2:567550/140‑1 (MQ=255)
                                                           ccgAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCAt                                                                        <  2:303236/140‑1 (MQ=255)
                                                                cACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCcgc                                                                   >  1:1251013/1‑140 (MQ=255)
                                                                  ccATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGt                                                                 <  2:717580/140‑1 (MQ=255)
                                                                           tGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGc                                                        >  2:846337/1‑140 (MQ=255)
                                                                                  tttCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGAc                                                 >  2:1011311/1‑140 (MQ=255)
                                                                                  tttCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGAc                                                 <  1:460342/140‑1 (MQ=255)
                                                                                          ccACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTgaggag                                         <  1:846337/140‑1 (MQ=255)
                                                                                              tGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGgcgc                                     <  1:1011311/140‑1 (MQ=255)
                                                                                              tGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGgcgc                                     <  2:225414/140‑1 (MQ=255)
                                                                                              tGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGgcgc                                     <  1:29253/140‑1 (MQ=255)
                                                                                                 cAAAAACCACGCGTACATCCAGTGCGGCGTTAACAAACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGcc                                  <  2:256401/140‑1 (MQ=255)
                                                                                                    aaaCCACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAgg                               >  2:181249/1‑140 (MQ=255)
                                                                                                       ccACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGgtgt                            <  1:716796/140‑1 (MQ=255)
                                                                                                        cACGCGTACATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTa                           <  2:348923/140‑1 (MQ=255)
                                                                                                                cATCCAGTGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGt                   <  1:538055/140‑1 (MQ=255)
                                                                                                                       tGCGGCGTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTa            >  1:969209/1‑140 (MQ=255)
                                                                                                                        gcggcgTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAg           >  1:1177286/1‑140 (MQ=255)
                                                                                                                         cggcgTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAgc          >  2:708257/1‑140 (MQ=255)
                                                                                                                            cgTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGca       >  2:557258/1‑140 (MQ=255)
                                                                                                                            cgTTAACATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGca       >  2:1017640/1‑140 (MQ=255)
                                                                                                                                 aCATACCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGcagccag  <  1:311437/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
TCAGCCTGCGCCACAGCAGTCCTGCGCCGCCAATCAACGTCAACACACCGCAAATACCGCCGAGCACCATCGCCATGAGTTGTTTCGCTGCCACTGGCAAAAACCACGCGTACATCCAGTGCGGCGTTAACATGCCGAACAAGTGCCCGAAGAAAATCCCCAAAATGCCGATATGGAACAGATTCGACCATATCACCATCCCGCGTTTATCGAGCATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAG  >  NC_000913/1536275‑1536543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A