Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,679,346	C→T	T307I (ACC→ATC)	ydgH →	DUF1471 domain‑containing protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,679,346	0	C	T	100.0%	187.9 / NA	53	T307I (ACC→ATC)	ydgH	DUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/25); total (28/25)

TTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCG  >  NC_000913/1679210‑1679476

ttCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGt                                                                                                                                 <  2:1124137/140‑1 (MQ=255)

ttCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGt                                                                                                                                 >  1:140532/1‑140 (MQ=255)

ttCGACAGAATAAACTTCTCTGGCAACTACGCCAACATGACCGAACTCTCCTATCAGGTTGCGAAGCGTGCCGAAAAAAAAGGTGCTAATTATTCCCCCAACCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGt                                                                                                                                 <  2:1280827/140‑1 (MQ=255)

     cAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAgcg                                                                                                                            <  2:77873/140‑1 (MQ=255)

       ccATCAAATTCTCTGGCAACTCCGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCAAAAAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCa                                                                                                                          <  2:843611/139‑1 (MQ=255)

        cATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAg                                                                                                                         <  2:906701/140‑1 (MQ=255)

         ataaaaTTCTCGGGCAACTACGGCAACATGACCGAATTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTCCCCCATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGa                                                                                                                        <  2:1230225/137‑1 (MQ=255)

           aaaaTTCTCTGGCAACCACGAAAAAATAACCAAAGTTTCCTATCAGGTTGCGAAACGTGCCGGGAAGAAAGGTGCTAAGTATTCCCACCACCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATc                                                                                                                      <  2:176780/139‑1 (MQ=255)

                    tGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAgcg                                                                                                                            <  2:362344/125‑1 (MQ=255)

                    tGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAgcg                                                                                                                            >  1:362344/1‑125 (MQ=255)

                    tGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTataaat                                                                                                             <  1:18648/140‑1 (MQ=255)

                    agacagcTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAACCGTGCCGCAAAGAAGGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTataaat                                                                                                             <  2:1224412/134‑1 (MQ=255)

                      gCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTataaataa                                                                                                           >  2:211750/1‑140 (MQ=255)

                            ccGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTc                                                                                                     <  2:825355/139‑1 (MQ=255)

                               gCAACATGCCCGAAGTCTCCTATCAGGTTGCAAAACGTCCCGCGAAAAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGt                                                                                                  <  2:834671/140‑1 (MQ=255)

                                 aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTa                                                                                                <  1:435931/140‑1 (MQ=255)

                                 aaCAGGGCCGAAGTCTCCTTTCAGGTTGGAAAAGGTGCCGAGAAAAAGGGTGTTAAGTATTACCACAGCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTa                                                                                                <  2:270484/140‑1 (MQ=255)

                                   cATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACt                                                                                              >  1:834671/1‑140 (MQ=255)

                                    aTGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTc                                                                                             >  2:404109/1‑140 (MQ=255)

                                     tGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGtctgtct                                                                                            >  1:1224412/1‑134 (MQ=255)

                                              tctcCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAggcg                                                                                   >  2:171470/1‑140 (MQ=255)

                                                            gCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCt                                                                     >  1:1059666/1‑140 (MQ=255)

                                                            gCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACCCCTATAAGGCGGCTTGATGCCGCCt                                                                     >  2:415256/1‑140 (MQ=255)

                                                               aaaCGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTc                                                                  >  1:1224041/1‑140 (MQ=255)

                                                                aaCGGGCCGCGAAAAAAGGTGCTAATTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCg                                                                 <  2:113300/140‑1 (MQ=255)

                                                                                           aTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGa                                      <  1:211750/140‑1 (MQ=255)

                                                                                           aTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGa                                      <  1:171470/140‑1 (MQ=255)

                                                                                           aTTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGa                                      <  2:1224041/140‑1 (MQ=255)

                                                                                            ttACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGAc                                     >  1:989853/1‑140 (MQ=255)

                                                                                            ttACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGAc                                     >  1:1244361/1‑140 (MQ=255)

                                                                                             tACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGAcc                                    >  2:428698/1‑140 (MQ=255)

                                                                                             tACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGAcc                                    >  1:149490/1‑140 (MQ=255)

                                                                                               ccACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAt                                  <  2:989853/140‑1 (MQ=255)

                                                                                                 acaTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACCCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtg                                <  1:415256/140‑1 (MQ=255)

                                                                                                  caTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCCGCGCCGGGCTGTCTAAATAACTATTCCGTTACTCCTATAAGGCGGCCTGATGCGGCCTTTCGCCTTTTTAGTCACAAATTTCCCCGCGACCCTtgc                               >  2:1192788/1‑140 (MQ=255)

                                                                                                  caTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgc                               >  2:752800/1‑140 (MQ=255)

                                                                                                  caTCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgc                               >  1:552315/1‑140 (MQ=255)

                                                                                                    tCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAt                                  >  2:223346/1‑135 (MQ=255)

                                                                                                    tCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCAt                                  <  1:223346/135‑1 (MQ=255)

                                                                                                    tCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcat                             <  1:428698/140‑1 (MQ=255)

                                                                                                    tCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcat                             >  1:685424/1‑140 (MQ=255)

                                                                                                    tCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcat                             >  2:749356/1‑140 (MQ=255)

                                                                                                      aCCCGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtgcatgc                           <  2:552315/140‑1 (MQ=255)

                                                                                                       cccGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtg                                <  2:1151188/134‑1 (MQ=255)

                                                                                                       cccGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATtg                                >  1:1151188/1‑134 (MQ=255)

                                                                                                        ccGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGAc                                     <  2:463934/128‑1 (MQ=255)

                                                                                                        ccGCCAGTGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGAc                                     >  1:463934/1‑128 (MQ=255)

                                                                                                               tGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACAtc                  >  1:596368/1‑140 (MQ=255)

                                                                                                               tGGCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACAtc                  >  1:98806/1‑140 (MQ=255)

                                                                                                                 gCAGGAACGTGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACAtctc                >  1:976369/1‑140 (MQ=255)

                                                                                                                          tGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTaaaa       >  1:1037021/1‑140 (MQ=255)

                                                                                                                          tGGTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTaaaa       >  1:815558/1‑140 (MQ=255)

                                                                                                                           ggTAATAACCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAAt      >  2:628124/1‑140 (MQ=255)

                                                                                                                               ataaCCTGATCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCcg  >  2:482819/1‑140 (MQ=255)

TTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAAGGCGGCTTGATGCCGCCTTTCGCATTTTTAGTCACAAATTTCCCCGCGACCATTGCATGCAGACACATCTCTTCGTAAAATCCCG  >  NC_000913/1679210‑1679476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A