Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,083	A→G	F218L (TTT→CTT)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,083	0	A	G	100.0%	175.6 / NA	48	F218L (TTT→CTT)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/19); total (29/19)

AGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACG  >  NC_000913/1828952‑1829214

aGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAAc                                                                                                                             >  1:248839/1‑140 (MQ=255)

    gcgTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTc                                                                                                                         >  2:305840/1‑140 (MQ=255)

    gcgTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTc                                                                                                                         >  2:1030907/1‑140 (MQ=255)

     cgTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCg                                                                                                                        >  1:1046773/1‑140 (MQ=255)

      gTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCga                                                                                                                       <  2:795937/140‑1 (MQ=255)

          atatCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGAtc                                                                                                                   <  1:954280/140‑1 (MQ=255)

            atCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGAtcct                                                                                                                 <  1:1030907/140‑1 (MQ=255)

                            aTAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAgcgc                                                                                                 >  1:841812/1‑140 (MQ=255)

                                ttAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGa                                                                                             >  1:345473/1‑140 (MQ=255)

                                  aGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGAcc                                                                                           >  2:972268/1‑140 (MQ=255)

                                  aGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGAcc                                                                                           >  2:551773/1‑140 (MQ=255)

                                  aGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGAcc                                                                                           <  1:551773/140‑1 (MQ=255)

                                    cGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCg                                                                                         >  2:961821/1‑140 (MQ=255)

                                       attattACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGttt                                                                                      <  2:809706/140‑1 (MQ=255)

                                             aCCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgcc                                                                                <  1:1216879/140‑1 (MQ=255)

                                                 cccATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgcccgcc                                                                            <  1:127196/140‑1 (MQ=255)

                                                 cccATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgcccgcc                                                                            >  2:881427/1‑140 (MQ=255)

                                                 cccATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgcccgcc                                                                            >  1:689986/1‑140 (MQ=255)

                                                 cccATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgcccgcc                                                                            >  1:175872/1‑140 (MQ=255)

                                                 cccATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgcccgcc                                                                            <  1:305840/140‑1 (MQ=255)

                                                   cATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCt                                                                          >  1:1000652/1‑140 (MQ=255)

                                                     tctcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgc                                                                                 >  1:1221386/1‑131 (MQ=255)

                                                     tctcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgc                                                                                 <  2:1221386/131‑1 (MQ=255)

                                                     tctcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGc                                                                        >  2:607054/1‑140 (MQ=255)

                                                     tctcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGc                                                                        >  2:480851/1‑140 (MQ=255)

                                                       tcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCAc                                                                      <  1:521240/140‑1 (MQ=255)

                                                                agaATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAg                                                             >  2:842550/1‑140 (MQ=255)

                                                                agaATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAg                                                             >  2:308795/1‑140 (MQ=255)

                                                                agaATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAACAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAg                                                             >  2:623711/1‑140 (MQ=255)

                                                                 gaATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCACACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGc                                                            >  2:730900/1‑140 (MQ=255)

                                                                       tctcCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCt                                                      <  2:841812/140‑1 (MQ=255)

                                                                       tctcCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAACAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCt                                                      <  1:623711/140‑1 (MQ=255)

                                                                              cTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCa                                               <  2:1153214/140‑1 (MQ=255)

                                                                                gACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAag                                             <  1:607054/140‑1 (MQ=255)

                                                                                 aCCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAagc                                            <  2:1046773/140‑1 (MQ=255)

                                                                                 aCCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAagc                                            >  1:1164114/1‑140 (MQ=255)

                                                                                           tGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCctgctg                                  >  1:837475/1‑140 (MQ=255)

                                                                                             gCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCACACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGcc                                <  1:730900/140‑1 (MQ=255)

                                                                                                aTGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATa                             >  1:1051395/1‑140 (MQ=255)

                                                                                                       tGGTAGCCTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCAt                      >  2:406117/1‑140 (MQ=255)

                                                                                                         gTAGCCTGTATTGGCGCTACCGGGAAGCAGCAAACCGTCGAGATCCGCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTa                    >  2:762059/1‑140 (MQ=255)

                                                                                                             ccTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGc                <  1:972268/140‑1 (MQ=255)

                                                                                                              cTGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCt               >  1:451835/1‑140 (MQ=255)

                                                                                                               tGTATTGGCGCTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCtt              <  1:308795/140‑1 (MQ=255)

                                                                                                                      gcgcTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACt       >  1:1074284/1‑140 (MQ=255)

                                                                                                                         cTACCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCa    <  2:248839/140‑1 (MQ=255)

                                                                                                                           aCCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACg  >  2:352028/1‑140 (MQ=255)

                                                                                                                           aCCGGTAAGCAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACg  >  1:768732/1‑140 (MQ=255)

AGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACG  >  NC_000913/1828952‑1829214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A