Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	258	142.3	4824480	99.1%	4781059	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,832,698	A→G	I91V (ATT→GTT)	xthA →	exodeoxyribonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,832,698	0	A	G	100.0%	338.2 / NA	94	I91V (ATT→GTT)	xthA	exodeoxyribonuclease III
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (36/58); total (36/58)

GGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCAGAATCTGCAA  >  NC_000913/1832565‑1832826

ggTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGttatta                                                                                                                             <  1:1039214/140‑1 (MQ=255)

 gTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTtattat                                                                                                                            >  1:470660/1‑140 (MQ=255)

 gTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTtattat                                                                                                                            <  2:1388830/140‑1 (MQ=255)

   ggCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATgg                                                                                                                          >  1:1428832/1‑140 (MQ=255)

   ggCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATgg                                                                                                                          >  2:294042/1‑140 (MQ=255)

    gCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGc                                                                                                                         >  1:2000103/1‑140 (MQ=255)

     cGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑g                                                                                                                       >  2:695033/1‑140 (MQ=255)

     cGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑g                                                                                                                       >  1:1214395/1‑140 (MQ=255)

     cGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑g                                                                                                                       <  1:2211238/140‑1 (MQ=255)

       aaGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGa                                                                                                                     <  2:231497/140‑1 (MQ=255)

       aaGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGa                                                                                                                     >  1:256595/1‑140 (MQ=255)

          cTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGT‑GGAAAt                                                                                                                  <  1:1438338/140‑1 (MQ=255)

          cTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAAt                                                                                                                  <  2:1972092/140‑1 (MQ=255)

          cTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAAt                                                                                                                  <  2:290038/140‑1 (MQ=255)

          cTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAAt                                                                                                                  <  2:372610/140‑1 (MQ=255)

           tCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCGATTATGACGTAGAGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTTTTATGGC‑GGGAATc                                                                                                                 >  2:2261903/1‑140 (MQ=255)

            cGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAAt                                                                                                                  >  1:546250/1‑138 (MQ=255)

            cGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAAt                                                                                                                  <  2:546250/138‑1 (MQ=255)

            cGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATcc                                                                                                                >  1:1039520/1‑140 (MQ=255)

            cGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGGAAAcc                                                                                                                >  2:278534/1‑140 (MQ=255)

              gCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATcccc                                                                                                              >  2:488898/1‑140 (MQ=255)

              gCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGGAAAcccc                                                                                                              >  2:1505824/1‑140 (MQ=255)

               cTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCt                                                                                                             <  1:982867/140‑1 (MQ=255)

               cTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCt                                                                                                             <  2:2105868/140‑1 (MQ=255)

               cTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCt                                                                                                             <  1:1313090/140‑1 (MQ=255)

               cTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCt                                                                                                             <  1:2009232/140‑1 (MQ=255)

                 aCAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCa                                                                                                           <  2:470660/140‑1 (MQ=255)

                 aCAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCa                                                                                                           <  1:2272384/140‑1 (MQ=255)

                  cAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCAc                                                                                                          <  1:934924/140‑1 (MQ=255)

                   aaCGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCAct                                                                                                         <  2:582104/140‑1 (MQ=255)

                    aCGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActg                                                                                                        >  1:1907646/1‑140 (MQ=255)

                    aCGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActg                                                                                                        <  1:488898/140‑1 (MQ=255)

                     ggtgtTTTATCACGGGCAGAAAGGAAATTATGGCGTGGAGCTGCTGACCGAAGAGACGCCGATCGCCGTGCGACTCGGCTTTCACGGTGACGACGAAGAGGGGCTGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgc                                                                                                       <  2:1629370/139‑1 (MQ=255)

                     cGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgc                                                                                                       <  2:177065/140‑1 (MQ=255)

                     cGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgc                                                                                                       <  1:77701/140‑1 (MQ=255)

                     cGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgc                                                                                                       <  2:2000103/140‑1 (MQ=255)

                       tgtTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgctg                                                                                                     <  1:648340/140‑1 (MQ=255)

                       tgtTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgctg                                                                                                     <  1:1833543/140‑1 (MQ=255)

                       tgtTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgctg                                                                                                     >  1:333846/1‑140 (MQ=255)

                       tgtTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCActgctg                                                                                                     <  2:1428832/140‑1 (MQ=255)

                         ttttATCGCGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTggg                                                                                                   <  1:2259656/140‑1 (MQ=255)

                         ttttATCACGGGCAGATAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTggg                                                                                                   <  2:2160422/140‑1 (MQ=255)

                         ttttATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAAACCCCCCACTGCTggg                                                                                                   >  2:796810/1‑140 (MQ=255)

                            tATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGGGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGCGGGAAACCCCCTC‑CCGCTGGGGaa                                                                                                >  2:934924/1‑140 (MQ=255)

                              tCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGCGGGAAATCCCC‑CCCCGCTGGGGAATg                                                                                              >  2:1514138/1‑140 (MQ=255)

                              tCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATg                                                                                              <  1:1957059/140‑1 (MQ=255)

                              tCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATg                                                                                              <  1:958211/140‑1 (MQ=255)

                                 cGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCa                                                                                           <  2:1484399/140‑1 (MQ=255)

                                  gggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGGAAACCCCTCCCTGCTGGGGAATGTTAc                                                                                          >  2:1957059/1‑140 (MQ=255)

                                  gggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGGAAACCCCCCACTTCTGGGGAATGTCAc                                                                                          >  2:958211/1‑140 (MQ=255)

                                   ggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGGAAACCCCCCCCTGCTGGGGAATGTCAcc                                                                                         >  2:2387374/1‑140 (MQ=255)

                                   ggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCAcc                                                                                         <  2:451383/140‑1 (MQ=255)

                                   ggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCAcc                                                                                         <  1:2125619/140‑1 (MQ=255)

                                   ggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCAcc                                                                                         <  2:949049/140‑1 (MQ=255)

                                   ggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGGAAAGTCAcc                                                                                         >  1:898743/1‑140 (MQ=255)

                                   ggCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGAAAACCCCCCCCCGCTGGGGAATGTCAcc                                                                                         >  2:1984014/1‑140 (MQ=255)

                                      aGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGGCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTg                                                                                      <  1:180276/140‑1 (MQ=255)

                                       gAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGa                                                                                     <  1:695033/140‑1 (MQ=255)

                                         aaGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATc                                                                                   <  1:294042/140‑1 (MQ=255)

                                         aaGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATc                                                                                   <  1:1505824/140‑1 (MQ=255)

                                           ggCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCaa                                                                                 <  2:1602071/140‑1 (MQ=255)

                                              cATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCCCTGCTGGGGAATGTCACCGCGATCAACgg                                                                              >  1:370934/1‑140 (MQ=255)

                                              cATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACgg                                                                              <  2:140425/140‑1 (MQ=255)

                                                   tggcgtggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACt                                                                         <  1:400299/140‑1 (MQ=255)

                                                      cgtggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGGAAACCCCCCCCCTCCGGGGAAAGTCACCCGGGTCAAAGGGTACCTcc                                                                      >  2:520264/1‑140 (MQ=255)

                                                      cgtggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGAAATCCCCCCACTGCTGGGGAAAGTCACCCTGATCAACGGGTACTTcc                                                                      >  2:375357/1‑140 (MQ=255)

                                                        tggcgCTGCTGACCAAAGAGACGCCGGTTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTA‑GGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGc                                                                   <  2:1416238/140‑1 (MQ=255)

                                                        tggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAAACCCCCCCCTGCTGGGGAAAGTCACCGGGGTTAAAGGGTACTTtccc                                                                    >  2:445247/1‑139 (MQ=255)

                                                         ggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGc                                                                   <  1:872733/140‑1 (MQ=255)

                                                         ggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGc                                                                   <  1:2261903/140‑1 (MQ=255)

                                                         ggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGc                                                                   <  1:2183975/140‑1 (MQ=255)

                                                         ggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGc                                                                   <  1:375357/140‑1 (MQ=255)

                                                         ggcgCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGc                                                                   <  1:518775/140‑1 (MQ=255)

                                                          gcgcTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGGAAACCCCTCCCTGCTGGGGGATGTNACCCGGGTCAAAGGGTACTTcccccc                                                                  >  2:400299/1‑137 (MQ=255)

                                                          gcgcTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGAAAACCCCCCACTGCTGGGGAATGTCACCCTGATCAACGGGTACTTtccccc                                                                  >  2:50293/1‑137 (MQ=255)

                                                                ctgACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GTAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGa                                                            >  1:1161152/1‑140 (MQ=255)

                                                                ctgACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGa                                                            <  1:50293/140‑1 (MQ=255)

                                                                        agagACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCCCACTGCTGGGGAATGTCACCCTGATCAACGGGTACTTCCCGCAGGGGGAAAGCcgcg                                                    >  2:925521/1‑140 (MQ=255)

                                                                          agaCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGGGAAAGCCGCGAc                                                  >  1:2039178/1‑140 (MQ=255)

                                                                            aCGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCa                                                <  2:1907646/140‑1 (MQ=255)

                                                                              gCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATc                                              <  2:1039520/140‑1 (MQ=255)

                                                                              gCCGATTGCCGTGCGTCCCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACTGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATc                                              <  2:2143613/140‑1 (MQ=255)

                                                                                      ccGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGGAATGTCACCGTGATCAACGGGTACTTCCCGCAGGGGGAAAACCGCGACCATCCGATaaaa                                      >  1:1421366/1‑140 (MQ=255)

                                                                                         tGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGGAATGTCACCGTGATCAACGGGTACTTCCCGCAGGGGGAAAACCGCGACCATCCGATAAAAATc                                   >  1:1556486/1‑140 (MQ=255)

                                                                                           cGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCCCACTGCTGGGGAATGTCACCGGGGTCAACGGGTACTTCCCCCAGGGGGAAAGCCGCGACCCTCCGATAAAATTccc                                 >  2:16251/1‑140 (MQ=255)

                                                                                             tCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCgg                               <  1:1984014/140‑1 (MQ=255)

                                                                                                cgGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGGGTATTATGGC‑GGAAAACCCCTCCCTGCCGGGGTATGTCACCGTGATCAACGGGTACTTCCCGCAGGGGGGAAGCCGCGACCCTCCCATAAAATTCCCGGCaa                            >  2:560129/1‑140 (MQ=255)

                                                                                                      tCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCACCGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgcgc                      <  2:1668343/140‑1 (MQ=255)

                                                                                                      tCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgcgc                      <  1:574493/140‑1 (MQ=255)

                                                                                                      tCCCGGTGACGACGAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAgcgc                      <  1:2365346/140‑1 (MQ=255)

                                                                                                              acgacgAAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTAt              <  1:118956/140‑1 (MQ=255)

                                                                                                                 acgaAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCCCACTGCTGGGGAATGTCACCGGGATCAACGGGTACTTCCCCCAGGGGGAAAACCGCGACCATCCCATAAAAATCCCGGCAAAAGCGCAGGTTTATCAg           >  2:2323863/1‑140 (MQ=255)

                                                                                                                  cgaAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCGCCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCAGa          <  2:498247/140‑1 (MQ=255)

                                                                                                                  cgaAGAGGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCAGa          <  1:925521/140‑1 (MQ=255)

                                                                                                                       agGCGCAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCAGAATCTg     <  2:333846/140‑1 (MQ=255)

                                                                                                                         gcgcAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCAGAATCTGCa   <  1:560129/140‑1 (MQ=255)

                                                                                                                          cgcAGCGGCGGGTTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGGGATCAACGGTTACTTCCCGCAGGGGGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCAGAATCTGCaa  >  1:485892/1‑140 (MQ=255)

GGTGGCGAAGCTCGGCTACAACGTGTTTTATCACGGGCAGAAAGGCCATTATGGCGTGGCGCTGCTGACCAAAGAGACGCCGATTGCCGTGCGTCGCGGCTTTCCCGGTGACGACGAAGAGGCGCAGCGGCGGATTATTATGGC‑GGAAATCCCCTCACTGCTGGGTAATGTCACCGTGATCAACGGTTACTTCCCGCAGGGTGAAAGCCGCGACCATCCGATAAAATTCCCGGCAAAAGCGCAGTTTTATCAGAATCTGCAA  >  NC_000913/1832565‑1832826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A