Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F28 I1 R1
|
161 |
75.5 |
2581324 |
99.3% |
2563254 |
138.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,904,787 |
(T)7→8 |
intergenic (+41/‑14) |
manZ → / → yobD |
mannose‑specific PTS enzyme IID component/conserved inner membrane protein YobD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,904,780 | 1 | . | T | 98.2%
| 192.5
/ ‑3.5
| 56 | intergenic (+34/‑21) | manZ/yobD | mannose‑specific PTS enzyme IID component/conserved inner membrane protein YobD |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (28/27); total (29/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
ATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCG‑TTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAAT > NC_000913/1904648‑1904910
|
aTGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCG‑ttttt‑tt > 2:1065645/1‑140 (MQ=255)
aTGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCG‑ttttt‑tt > 1:234709/1‑140 (MQ=255)
tgtgGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCG‑ttttt‑ttt < 1:443148/140‑2 (MQ=255)
tACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTgg < 1:976129/140‑1 (MQ=255)
cTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCGTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGGCGTTAGGCCGCG‑TTTTTGTTATCTggag > 2:898550/1‑140 (MQ=255)
cTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTggag > 1:1132614/1‑140 (MQ=255)
cTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTggag > 2:70652/1‑140 (MQ=255)
cgcAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGAt > 1:1100667/1‑140 (MQ=255)
cgcAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGAt > 1:39625/1‑140 (MQ=255)
gcAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGAtt < 1:99452/140‑1 (MQ=255)
gcAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGAtt < 1:562824/140‑1 (MQ=255)
cAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTa > 2:25652/1‑140 (MQ=255)
aaaaaaGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTaa < 2:891552/140‑1 (MQ=255)
aaaaaGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCACCGTTTTTT‑TTATCTGGAGGATTAAt > 1:902668/1‑140 (MQ=255)
aCCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCAc > 1:309038/1‑140 (MQ=255)
aCCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCAc < 2:1132614/140‑1 (MQ=255)
aCCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCAc < 1:898550/140‑1 (MQ=255)
cGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGa < 2:309038/140‑1 (MQ=255)
gCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGAc < 2:39625/140‑1 (MQ=255)
gCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGAc < 1:61682/140‑1 (MQ=255)
cTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCa < 2:869789/134‑1 (MQ=255)
cTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCa > 1:869789/1‑134 (MQ=255)
cTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGAcc < 1:1194790/140‑1 (MQ=255)
gATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTa < 2:234709/140‑1 (MQ=255)
atcatcGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTAc < 2:1100667/140‑1 (MQ=255)
atcatcGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTAc > 2:608451/1‑140 (MQ=255)
atcGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGa > 2:2068/1‑140 (MQ=255)
tcGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTGGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGAt > 1:1126777/1‑140 (MQ=255)
cGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGAtt > 2:282721/1‑140 (MQ=255)
cGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACACTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGAtt > 1:987156/1‑140 (MQ=255)
ttGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCt > 2:296514/1‑140 (MQ=255)
ttGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCt > 1:292130/1‑140 (MQ=255)
ttcGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcg > 2:752405/1‑140 (MQ=255)
ttcGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcg > 2:1111644/1‑140 (MQ=255)
ttcGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcg > 1:432996/1‑140 (MQ=255)
tcGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgc < 1:755661/140‑1 (MQ=255)
tcGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgc > 2:755661/1‑140 (MQ=255)
gTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGTCTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgccg < 1:636155/140‑1 (MQ=255)
gTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgccg < 2:1126777/140‑1 (MQ=255)
gTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgccg < 1:282721/140‑1 (MQ=255)
gTCATCGGTATCGCTGGTTACACTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATcgccg < 2:987156/140‑1 (MQ=255)
ggTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTcc < 2:299277/140‑1 (MQ=255)
gTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCt < 1:1007196/140‑1 (MQ=255)
tGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTc < 1:320798/140‑1 (MQ=255)
ggTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcg < 1:2068/140‑1 (MQ=255)
gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc > 1:927172/1‑140 (MQ=255)
gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc < 1:1065645/140‑1 (MQ=255)
gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc > 1:528911/1‑140 (MQ=255)
gTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTcgc < 2:528911/140‑1 (MQ=255)
aCGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGAt < 1:608451/140‑1 (MQ=255)
tGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACg < 2:432996/140‑1 (MQ=255)
cGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGAt < 1:1229289/140‑1 (MQ=255)
tAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGcccc > 1:854026/1‑140 (MQ=255)
gtACACTACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACgg > 2:81584/1‑140 (MQ=255)
tACCGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCAc > 1:985694/1‑140 (MQ=255)
ccGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCAccc > 2:523733/1‑140 (MQ=255)
ccGGGGCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCAccc > 1:527494/1‑140 (MQ=255)
gggCCTTTTGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCctgc > 2:934529/1‑140 (MQ=255)
ttGGCCCCGTTTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAAt > 1:288548/1‑140 (MQ=255)
|
ATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTGTACACTACCGGGGCCTTTTGGCCCCG‑TTTTT‑TTATCTGGAGGATTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAAT > NC_000913/1904648‑1904910
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
