Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,906,058	T→C	V124A (GTT→GCT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,906,058	0	T	C	100.0%	134.2 / NA	39	V124A (GTT→GCT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/21); total (18/21)

CGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGC  >  NC_000913/1905927‑1906184

cggcggGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTg                                                                                                                        >  1:886227/1‑140 (MQ=255)

       cgAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCt                                                                                                                 <  1:91516/140‑1 (MQ=255)

        gAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTg                                                                                                                <  2:966609/140‑1 (MQ=255)

        gAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTg                                                                                                                <  2:886227/140‑1 (MQ=255)

             attattGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGt                                                                                                           <  1:569061/140‑1 (MQ=255)

               tattGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTg                                                                                                                <  1:262618/133‑1 (MQ=255)

               tattGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTg                                                                                                                >  2:262618/1‑133 (MQ=255)

               tattGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCa                                                                                                         <  1:712536/140‑1 (MQ=255)

                attGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCaa                                                                                                        >  1:516045/1‑140 (MQ=255)

                      ggTTCTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTAt                                                                                                  >  2:846414/1‑140 (MQ=255)

                        ttttCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATcg                                                                                                >  1:418332/1‑140 (MQ=255)

                                 cgcAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGGCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCAt                                                                                       <  2:654075/140‑1 (MQ=255)

                                  gcAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCAtt                                                                                      >  1:26800/1‑140 (MQ=255)

                                   cAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTg                                                                                     >  2:112435/1‑140 (MQ=255)

                                       tgatgaAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCa                                                                                 <  2:124321/140‑1 (MQ=255)

                                       tgatgaAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCa                                                                                 <  2:485317/140‑1 (MQ=255)

                                       tgatgaAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCa                                                                                 <  2:418332/140‑1 (MQ=255)

                                                tgaagaGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg                                                                        >  1:808654/1‑140 (MQ=255)

                                                tgaagaGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg                                                                        <  2:808654/140‑1 (MQ=255)

                                                tgaagaGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg                                                                        <  1:554876/140‑1 (MQ=255)

                                                tgaagaGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg                                                                        >  2:131984/1‑140 (MQ=255)

                                                    gagCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAAc                                                                    <  1:205907/140‑1 (MQ=255)

                                                          cgcCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGAt                                                              <  2:516045/140‑1 (MQ=255)

                                                            ccGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGc                                                                       >  1:156563/1‑129 (MQ=255)

                                                            ccGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGc                                                                       <  2:156563/129‑1 (MQ=255)

                                                            ccGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTa                                                            >  1:800540/1‑140 (MQ=255)

                                                            ccGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTa                                                            >  2:648263/1‑140 (MQ=255)

                                                               tCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGt                                                         <  1:112435/140‑1 (MQ=255)

                                                                      ggTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACAtt                                                  >  1:383097/1‑140 (MQ=255)

                                                                       gTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTa                                                 <  2:383097/140‑1 (MQ=255)

                                                                       gTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTa                                                 <  1:648263/140‑1 (MQ=255)

                                                                             ggCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGgatg                                           >  1:176818/1‑140 (MQ=255)

                                                                                   tGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGAtggtt                                     <  2:17265/140‑1 (MQ=255)

                                                                                              gcgATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTGAACATTAGGGATGATGGTTGGTCGCTTTAt                          >  2:625735/1‑140 (MQ=255)

                                                                                              gcgATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTAt                          <  1:131984/140‑1 (MQ=255)

                                                                                                 aTTGCCACCAGCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCgg                       <  1:846414/140‑1 (MQ=255)

                                                                                                           gCCTGGATGCCATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAattatt             >  2:1017905/1‑140 (MQ=255)

                                                                                                                     cATGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATAGGCTCAATTATTGGGAAAAAAg   >  1:853735/1‑140 (MQ=255)

                                                                                                                      aTGGCTGTGGGTGCTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGc  >  2:462822/1‑140 (MQ=255)

CGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGC  >  NC_000913/1905927‑1906184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A