Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,719	T→C	V280A (GTC→GCC)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,719	0	T	C	100.0%	194.0 / NA	54	V280A (GTC→GCC)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/28); total (26/28)

GCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCG  >  NC_000913/1931582‑1931853

gCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGcct                                                                                                                                      >  2:871300/1‑140 (MQ=255)

gCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGcct                                                                                                                                      >  2:231724/1‑140 (MQ=255)

      acaGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTc                                                                                                                                >  1:731094/1‑140 (MQ=255)

        aGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGt                                                                                                                              >  1:1152084/1‑140 (MQ=255)

             aTGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCcaca                                                                                                                         <  1:1048691/140‑1 (MQ=255)

             aTGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCcaca                                                                                                                         <  1:1218013/140‑1 (MQ=255)

                  cccGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATa                                                                                                                    >  1:1048346/1‑140 (MQ=255)

                  cccGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATa                                                                                                                    <  2:1048346/140‑1 (MQ=255)

                    cGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATAcc                                                                                                                  >  1:201673/1‑140 (MQ=255)

                      cTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGt                                                                                                                              >  2:933962/1‑126 (MQ=255)

                      cTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGt                                                                                                                              <  1:933962/126‑1 (MQ=255)

                      cTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCgg                                                                                                                >  1:33749/1‑140 (MQ=255)

                                     gcgcAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAAt                                                                                                 <  1:543979/140‑1 (MQ=255)

                                     gcgcAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAAt                                                                                                 <  1:231724/140‑1 (MQ=255)

                                     gcgcAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAAt                                                                                                 >  1:897147/1‑140 (MQ=255)

                                       gcAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAAttt                                                                                               <  2:358107/140‑1 (MQ=255)

                                         aGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTtct                                                                                             <  2:921079/140‑1 (MQ=255)

                                             gaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAg                                                                                         <  2:18986/140‑1 (MQ=255)

                                             gaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAg                                                                                         <  2:102659/140‑1 (MQ=255)

                                             gaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAg                                                                                         <  2:470766/140‑1 (MQ=255)

                                             gaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAg                                                                                         <  2:752918/140‑1 (MQ=255)

                                             gaTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAg                                                                                         <  2:610672/140‑1 (MQ=255)

                                              aTTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGa                                                                                        >  2:359471/1‑140 (MQ=255)

                                                  cccGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAg                                                                                         <  2:169573/135‑1 (MQ=255)

                                                  cccGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAg                                                                                         >  1:169573/1‑135 (MQ=255)

                                                    cGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGAtctctc                                                                                  <  1:871300/140‑1 (MQ=255)

                                                       aaaGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCaga                                                                               >  1:135745/1‑140 (MQ=255)

                                                              tgCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGcc                                                                        <  1:359471/140‑1 (MQ=255)

                                                                  ggCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGc                                                                    >  2:1258345/1‑140 (MQ=255)

                                                                   gCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCa                                                                   <  2:897147/140‑1 (MQ=255)

                                                                        gggCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTAc                                                              >  1:748055/1‑140 (MQ=255)

                                                                        gggCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTAc                                                              >  1:429852/1‑140 (MQ=255)

                                                                        gggCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTAc                                                              >  2:1244034/1‑140 (MQ=255)

                                                                                   ggTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTc                                                   >  2:442263/1‑140 (MQ=255)

                                                                                        tttGGTGTCGGGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACt                                              >  2:10854/1‑140 (MQ=255)

                                                                                        tttGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACt                                              >  1:1241718/1‑140 (MQ=255)

                                                                                        tttGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACt                                              >  1:1116345/1‑140 (MQ=255)

                                                                                                  aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGc                                    >  2:572774/1‑140 (MQ=255)

                                                                                                   gCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCg                                   <  1:1258345/140‑1 (MQ=255)

                                                                                                   gCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCg                                   <  1:442263/140‑1 (MQ=255)

                                                                                                   gCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATCTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCg                                   <  2:1152084/140‑1 (MQ=255)

                                                                                                     tATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCggg                                 <  2:618836/140‑1 (MQ=255)

                                                                                                      aTTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGa                                <  2:1241718/140‑1 (MQ=255)

                                                                                                       tttGTCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGAt                               >  1:930722/1‑140 (MQ=255)

                                                                                                           tCTGTGGTGATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGt                           <  2:748055/140‑1 (MQ=255)

                                                                                                                ggtgATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTa                      <  2:930722/140‑1 (MQ=255)

                                                                                                                 gtgATGAGGTGATTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTAt                     >  1:92526/1‑140 (MQ=255)

                                                                                                                            aTTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGc          >  2:1016375/1‑140 (MQ=255)

                                                                                                                            aTTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGc          >  1:879083/1‑140 (MQ=255)

                                                                                                                            aTTTTCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGc          >  1:841205/1‑140 (MQ=255)

                                                                                                                              tttCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCtt        >  2:1230645/1‑140 (MQ=255)

                                                                                                                               ttCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc       <  2:271866/140‑1 (MQ=255)

                                                                                                                               ttCAGTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc       <  2:879083/140‑1 (MQ=255)

                                                                                                                                   gTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATgg                   <  1:90613/124‑1 (MQ=255)

                                                                                                                                   gTGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATgg                   >  2:90613/1‑124 (MQ=255)

                                                                                                                                    tGAGGCCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCg  <  1:707523/140‑1 (MQ=255)

GCAACCACAGCAAATGAGCCCGCTTGCCCTTGAACGTGCGCAGGAGATTGCCCGTAAAGTGGTGCTGGCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCG  >  NC_000913/1931582‑1931853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A