Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,295,140	T→C	T81A (ACC→GCC)	ccmE ←	periplasmic heme chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,295,140	0	T	C	100.0%	277.6 / NA	75	T81A (ACC→GCC)	ccmE	periplasmic heme chaperone
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (46/29); total (46/29)

TTCTTTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGTCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAG  >  NC_000913/2295006‑2295273

ttcttTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACtt                                                                                                                                  >  1:467125/1‑140 (MQ=255)

  cttTCGTGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTc                                                                                                                                >  2:1261741/1‑140 (MQ=255)

    ttCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAg                                                                                                                              <  1:755973/140‑1 (MQ=255)

    ttCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAg                                                                                                                              <  2:728942/140‑1 (MQ=255)

        cgAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGaa                                                                                                                          >  1:33473/1‑140 (MQ=255)

              tatGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGa                                                                                                                    <  1:427484/140‑1 (MQ=255)

                tGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATc                                                                                                                  <  2:455205/140‑1 (MQ=255)

                 gATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATcg                                                                                                                 <  2:467125/140‑1 (MQ=255)

                     gCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCt                                                                                                             >  1:952655/1‑140 (MQ=255)

                      ccTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTg                                                                                                            >  1:685156/1‑140 (MQ=255)

                       cTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATcgcg                                                                                                               <  2:143743/136‑1 (MQ=255)

                       cTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATcgcg                                                                                                               >  1:143743/1‑136 (MQ=255)

                       cTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGc                                                                                                           >  2:678172/1‑140 (MQ=255)

                       cTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGc                                                                                                           >  2:488899/1‑140 (MQ=255)

                              cAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTAc                                                                                                    <  1:172050/140‑1 (MQ=255)

                                gTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCCCTTTCAGCGAATTGGGATCGCGCTGCACACTAccc                                                                                                  <  2:640230/140‑1 (MQ=255)

                                  tCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCgg                                                                                                <  1:982660/140‑1 (MQ=255)

                                          caccacAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCa                                                                                        <  2:952655/140‑1 (MQ=255)

                                            ccacAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATc                                                                                      <  1:488899/140‑1 (MQ=255)

                                               caACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCg                                                                                   >  1:372185/1‑140 (MQ=255)

                                                   gccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCaa                                                                               <  2:33473/140‑1 (MQ=255)

                                                    ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAAc                                                                              >  1:1153048/1‑140 (MQ=255)

                                                    ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACAT‑CACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCAc                                                                                          <  2:42022/127‑1 (MQ=255)

                                                    ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACAT‑CACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCAc                                                                                          >  1:42022/1‑127 (MQ=255)

                                                       tgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAAcgcg                                                                           <  1:678172/140‑1 (MQ=255)

                                                            ttCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGAc                                                                      >  1:1258058/1‑140 (MQ=255)

                                                             tCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACg                                                                     <  1:1247292/140‑1 (MQ=255)

                                                                   aaCAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGAcc                                                               >  2:124727/1‑140 (MQ=255)

                                                                   aaCAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGAcc                                                               >  2:645373/1‑140 (MQ=255)

                                                                    aCAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGc                                                                    <  2:794091/134‑1 (MQ=255)

                                                                    aCAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGc                                                                    >  1:794091/1‑134 (MQ=255)

                                                                        aTCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACtt                                                          >  2:881425/1‑140 (MQ=255)

                                                                           cGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCg                                                       >  2:1022386/1‑140 (MQ=255)

                                                                            ggCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCgg                                                      >  1:90114/1‑140 (MQ=255)

                                                                             gCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGc                                                     >  2:521393/1‑140 (MQ=255)

                                                                             gCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGc                                                     >  1:537959/1‑140 (MQ=255)

                                                                               aaaaTGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCAt                                                   <  1:1261741/140‑1 (MQ=255)

                                                                                 aaTGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCACTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCAttt                                                 >  1:894624/1‑140 (MQ=255)

                                                                                  aTGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGc                                                     >  2:388841/1‑135 (MQ=255)

                                                                                  aTGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGc                                                     <  1:388841/135‑1 (MQ=255)

                                                                                   tGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGc                                               <  1:521393/140‑1 (MQ=255)

                                                                                       ttCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAg                                           >  2:483096/1‑140 (MQ=255)

                                                                                               agaCATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCTAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGc                                   <  2:1153048/140‑1 (MQ=255)

                                                                                               agaCATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGc                                   <  2:685156/140‑1 (MQ=255)

                                                                                                   aTCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGc                               >  1:1052870/1‑140 (MQ=255)

                                                                                                    tCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGcc                              >  1:333378/1‑140 (MQ=255)

                                                                                                     ccACTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCg                             >  2:995270/1‑140 (MQ=255)

                                                                                                       aCTGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTa                           <  2:372185/140‑1 (MQ=255)

                                                                                                         tGAGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGTCGTaga                         >  2:796355/1‑140 (MQ=255)

                                                                                                           aGCCTTCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAgaga                       >  1:1116481/1‑140 (MQ=255)

                                                                                                               ttCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAAttt                   >  2:501375/1‑140 (MQ=255)

                                                                                                                tCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTc                  >  1:801046/1‑140 (MQ=255)

                                                                                                                tCAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTc                  >  1:884221/1‑140 (MQ=255)

                                                                                                                 cAGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTcc                 >  2:1286311/1‑140 (MQ=255)

                                                                                                                  aGCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTccc                >  2:1231438/1‑140 (MQ=255)

                                                                                                                   gCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGTCGTAGAGAATTTcccc               <  1:796355/140‑1 (MQ=255)

                                                                                                                   gCATCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTcccc               <  1:1286311/140‑1 (MQ=255)

                                                                                                                     aTCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             <  2:333378/140‑1 (MQ=255)

                                                                                                                     aTCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             >  2:939959/1‑140 (MQ=255)

                                                                                                                     aTCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             <  1:414891/140‑1 (MQ=255)

                                                                                                                     aTCGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCACTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             <  2:894624/140‑1 (MQ=255)

                                                                                                                       cGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGc           >  1:929526/1‑140 (MQ=255)

                                                                                                                       cGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGc           >  1:643575/1‑140 (MQ=255)

                                                                                                                       cGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGc           >  2:850353/1‑140 (MQ=255)

                                                                                                                       cGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGc           >  2:356813/1‑140 (MQ=255)

                                                                                                                       cGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGc           >  1:897678/1‑140 (MQ=255)

                                                                                                                       cGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGc           >  1:396470/1‑140 (MQ=255)

                                                                                                                       cGTAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGCCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGc           >  2:701793/1‑140 (MQ=255)

                                                                                                                         tAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             >  2:742773/1‑136 (MQ=255)

                                                                                                                         tAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             <  1:742773/136‑1 (MQ=255)

                                                                                                                         tAAATGGTGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGt         >  2:475860/1‑140 (MQ=255)

                                                                                                                                tGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             <  1:586810/129‑1 (MQ=255)

                                                                                                                                tGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCg             >  2:586810/1‑129 (MQ=255)

                                                                                                                                tGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAag  >  1:550645/1‑140 (MQ=255)

                                                                                                                                tGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAag  >  1:935819/1‑140 (MQ=255)

                                                                                                                                tGAAGGCCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAag  <  1:1172335/140‑1 (MQ=255)

TTCTTTCGCGAGGATATGATTGCCTTTTTCCAGTTCGCCCTGCACCACAACGCCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTAAATGGTGAAGGTCACTTTCAGCGAATTGGGATCGCGCTGCACACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAG  >  NC_000913/2295006‑2295273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A