Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,646,629	A→G	S233P (TCC→CCC)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,646,629	0	A	G	100.0%	191.3 / NA	53	S233P (TCC→CCC)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/31); total (22/31)

GCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTA  >  NC_000913/2646493‑2646763

gCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCca                                                                                                                                     <  1:868388/140‑1 (MQ=255)

 cATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCcac                                                                                                                                    <  1:489898/140‑1 (MQ=255)

   tCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCcacac                                                                                                                                  <  2:941467/140‑1 (MQ=255)

   tCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCcacac                                                                                                                                  >  2:495343/1‑140 (MQ=255)

    ccAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCcacaca                                                                                                                                 <  2:1038158/140‑1 (MQ=255)

    ccAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCcacaca                                                                                                                                 <  2:847405/140‑1 (MQ=255)

           agtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCa                                                                                                                          >  2:562333/1‑140 (MQ=255)

            gtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCAc                                                                                                                         <  2:1238308/140‑1 (MQ=255)

            gtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCAc                                                                                                                         <  1:68391/140‑1 (MQ=255)

                tGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGc                                                                                                                           <  1:524509/134‑1 (MQ=255)

                tGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGc                                                                                                                           >  2:524509/1‑134 (MQ=255)

                 gATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTCCCTGCTCACGGGGCCACACACCTTGCACGGCCa                                                                                                                    <  1:724413/140‑1 (MQ=255)

                  aTTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCAt                                                                                                                   >  1:1139935/1‑140 (MQ=255)

                       gTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGtt                                                                                                              >  1:18169/1‑140 (MQ=255)

                        tCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTc                                                                                                             <  1:1055338/140‑1 (MQ=255)

                          gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGa                                                                                                           <  2:398928/140‑1 (MQ=255)

                          gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGa                                                                                                           <  1:797930/140‑1 (MQ=255)

                          gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGa                                                                                                           <  1:388088/140‑1 (MQ=255)

                          gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGa                                                                                                           <  1:896072/140‑1 (MQ=255)

                          gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGa                                                                                                           <  2:101202/140‑1 (MQ=255)

                             tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCGTCCGTTCGAGCa                                                                                                        <  1:1067917/140‑1 (MQ=255)

                                     tACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTttatta                                                                                                <  1:62481/140‑1 (MQ=255)

                                         gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGa                                                                                                           <  1:1287451/125‑1 (MQ=255)

                                         gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGa                                                                                                           >  2:1287451/1‑125 (MQ=255)

                                          aGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTAcgcg                                                                                            <  1:1057035/139‑1 (MQ=255)

                                          aGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTAcgcg                                                                                            >  2:1057035/1‑139 (MQ=255)

                                            catcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCATTCGAGCACTTTATTACGCGCgg                                                                                         <  2:743383/140‑1 (MQ=255)

                                              tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCt                                                                                       <  1:963962/140‑1 (MQ=255)

                                                 tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCg                                                                                    >  2:787185/1‑140 (MQ=255)

                                                 tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCg                                                                                    >  1:759776/1‑140 (MQ=255)

                                                 tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCg                                                                                    >  1:742116/1‑140 (MQ=255)

                                                      gAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACg                                                                               <  1:400718/140‑1 (MQ=255)

                                                        aaaCAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCt                                                                             >  2:1034304/1‑140 (MQ=255)

                                                        aaaCAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCt                                                                             <  2:346229/140‑1 (MQ=255)

                                                              cGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGcc                                                                       <  2:1044248/140‑1 (MQ=255)

                                                              cGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGcc                                                                       <  1:739199/140‑1 (MQ=255)

                                                                        tGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCgg                                                             <  2:18169/140‑1 (MQ=255)

                                                                           ggCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCg                                                          >  2:1219081/1‑140 (MQ=255)

                                                                           ggCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCg                                                          >  2:1219082/1‑140 (MQ=255)

                                                                           ggCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCg                                                          >  2:521716/1‑140 (MQ=255)

                                                                             cATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGaa                                                        >  1:635946/1‑140 (MQ=255)

                                                                               tttGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGa                                                      >  1:1044564/1‑140 (MQ=255)

                                                                               tttGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGa                                                      <  2:759776/140‑1 (MQ=255)

                                                                               tttGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGa                                                      <  2:1139935/140‑1 (MQ=255)

                                                                                          gccagccaGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGgcgc                                           >  2:839801/1‑140 (MQ=255)

                                                                                                   aTGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCaa                                  >  1:567233/1‑140 (MQ=255)

                                                                                                      ggTTCTTCCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAAc                               <  2:742116/140‑1 (MQ=255)

                                                                                                        ctcttcCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCg                             >  1:309225/2‑140 (MQ=255)

                                                                                                           ttcCCTTGACTCTTTTACCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCa                          >  2:1094171/1‑140 (MQ=255)

                                                                                                                      ctTTTCCCTGCTCACGGGGCCACACGCCTTGCCCGGCCATCCGTTCGACCTCTTTATTCCGCGCGGCTTCGGCCCGCCCCGGCCAACGACCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTgccgcc               <  2:1044564/140‑1 (MQ=255)

                                                                                                                           aCCTGCTCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGa          <  2:567233/140‑1 (MQ=255)

                                                                                                                                 tCACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCt    >  2:1218382/1‑140 (MQ=255)

                                                                                                                                  cACGGGGCCACACCCCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCtt   <  1:562333/140‑1 (MQ=255)

                                                                                                                                  cACGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCtt   >  2:393096/1‑140 (MQ=255)

                                                                                                                                   aCGGGGCCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTa  >  2:832136/1‑140 (MQ=255)

GCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGGCTTCGGCACGCTCCGGCCAACGATCCGGGCGAAGACGGCTGGGCGCTTGCGGCAAAACCGCCAGCATTGCCGCCTCGGAATAGCTTA  >  NC_000913/2646493‑2646763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A