Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,627,323	A→G	V739A (GTG→GCG)	rbbA ←	ribosome‑associated ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,627,323	0	A	G	100.0%	203.3 / NA	56	V739A (GTG→GCG)	rbbA	ribosome‑associated ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/30); total (26/30)

ACAGGCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCACCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATATTCATCAGCCCGCTT  >  NC_000913/3627192‑3627454

aCAGGCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTa                                                                                                                             >  1:40021/1‑140 (MQ=255)

 cAGGCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTaa                                                                                                                            <  1:1002746/140‑1 (MQ=255)

   ggCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAgg                                                                                                                          <  2:825831/140‑1 (MQ=255)

   ggCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAgg                                                                                                                          <  2:1021863/140‑1 (MQ=255)

   ggCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAgg                                                                                                                          <  2:606199/140‑1 (MQ=255)

    gCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAggg                                                                                                                         >  1:288823/1‑140 (MQ=255)

    gCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAggg                                                                                                                         >  2:603864/1‑140 (MQ=255)

      cGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGgcg                                                                                                                       <  2:288823/140‑1 (MQ=255)

           agCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTGTGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAg                                                                                                                  >  1:220868/1‑140 (MQ=255)

           agCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAg                                                                                                                  >  1:6897/1‑140 (MQ=255)

             cAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCa                                                                                                                <  2:102818/140‑1 (MQ=255)

                        tCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGatc                                                                                                     <  1:290015/140‑1 (MQ=255)

                                cccAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATTGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATcag                                                                                             <  2:688009/140‑1 (MQ=255)

                                cccAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATcag                                                                                             <  2:669115/140‑1 (MQ=255)

                                      gcgATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATcag                                                                                             <  2:638603/134‑1 (MQ=255)

                                      gcgATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATcag                                                                                             >  1:638603/1‑134 (MQ=255)

                                          tGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCggg                                                                                   >  1:278329/1‑140 (MQ=255)

                                          tGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCggg                                                                                   >  1:604383/1‑140 (MQ=255)

                                            tATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGAt                                                                                 <  1:946882/140‑1 (MQ=255)

                                                  aaCTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCAccgc                                                                           >  1:1190421/1‑140 (MQ=255)

                                                  aaCTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCAccgc                                                                           <  1:644663/140‑1 (MQ=255)

                                                    cTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCAccgccg                                                                         >  1:1044713/1‑140 (MQ=255)

                                                         tACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTACCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACa                                                                    >  2:138812/1‑140 (MQ=255)

                                                            cAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATc                                                                 >  1:357113/1‑140 (MQ=255)

                                                                  aaaaTTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGc                                                           <  1:382084/140‑1 (MQ=255)

                                                                  aaaaTTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGc                                                           <  2:1190421/140‑1 (MQ=255)

                                                                     aTTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTACCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAgg                                                        <  1:138812/140‑1 (MQ=255)

                                                                          cTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCtttt                                                   >  2:796244/1‑140 (MQ=255)

                                                                                gtggtgGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTc                                             <  1:265826/140‑1 (MQ=255)

                                                                                 tggtggGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTcc                                            <  2:40021/140‑1 (MQ=255)

                                                                                   gtggGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCgg                                          <  1:213308/140‑1 (MQ=255)

                                                                                     gggggTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAgg                                                        <  1:624989/124‑1 (MQ=255)

                                                                                     gggggTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAgg                                                        >  2:624989/1‑124 (MQ=255)

                                                                                       gggTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTta                                      <  1:410369/140‑1 (MQ=255)

                                                                                       gggTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTta                                      >  1:680251/1‑140 (MQ=255)

                                                                                            aCGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTatagcg                                 >  2:649544/1‑140 (MQ=255)

                                                                                              gTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTatagcgat                               >  2:386102/1‑140 (MQ=255)

                                                                                              gTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTatagcgat                               >  1:947499/1‑140 (MQ=255)

                                                                                                aaaGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGt                                              >  2:939946/1‑123 (MQ=255)

                                                                                                aaaGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGt                                              <  1:939946/123‑1 (MQ=255)

                                                                                                  aGGTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTatagcgatagcg                           <  1:760874/140‑1 (MQ=255)

                                                                                                    gTTGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGcgcg                         <  2:680251/140‑1 (MQ=255)

                                                                                                     ttGATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGt                        <  1:603864/140‑1 (MQ=255)

                                                                                                       gATAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGtct                      <  2:947499/140‑1 (MQ=255)

                                                                                                        aTAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGcgcg                         <  1:750570/136‑1 (MQ=255)

                                                                                                        aTAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGcgcg                         >  2:750570/1‑136 (MQ=255)

                                                                                                        aTAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGtctc                     >  2:375118/1‑140 (MQ=255)

                                                                                                         tAATCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCa                    <  2:1044713/140‑1 (MQ=255)

                                                                                                           aTCGACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAat                  <  1:1074324/140‑1 (MQ=255)

                                                                                                              gACCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATAtt               >  1:514477/1‑140 (MQ=255)

                                                                                                               aCCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTatagcgatagcg                           >  1:279811/1‑127 (MQ=255)

                                                                                                               aCCCAAGCTCTTTTTCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTatagcgatagcg                           <  2:279811/127‑1 (MQ=255)

                                                                                                                          ttttCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATATTCATCAGCCCGCt   >  2:748005/1‑140 (MQ=255)

                                                                                                                           tttCCCGCGCCACGCTAAGGGCGCTTAGTATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATATTCATCAGCCCGCtt  >  2:565723/1‑140 (MQ=255)

                                                                                                                           tttCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATATTCATCAGCCCGCtt  <  1:946607/140‑1 (MQ=255)

                                                                                                                           tttCCCGCGCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATATTCATCAGCCCGCtt  >  2:946607/1‑140 (MQ=255)

                                                                                                                                cgcgCCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATATTCATCAGcc       <  2:917817/130‑1 (MQ=255)

ACAGGCCGCAGAGCAGGAAAAAGTTCAGCATCCCCAGCGCGATGTATGGCAACTGTTTACCAAGCAAAAATTCACTACGCGTGGTGGGGGTCACGTAAAGGTTGATAATCGACCCAAGCTCTTTTTCCCGCACCACGCTAAGGGCGCTTAGCATTGACGGGATCATCATCAGCAGAAGCGGGATCACCGCCGGAACAATCGCTGGCAGGCTTTTTACGTCCGGGTTATAGCGATAGCGCGTCTCAATATTCATCAGCCCGCTT  >  NC_000913/3627192‑3627454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A