Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,739,251	C→T	G585G (GGC→GGT)	malS →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,739,251	0	C	T	100.0%	231.4 / NA	68	G585G (GGC→GGT)	malS	alpha‑amylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (37/31); total (37/31)

ATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTCGCGCACTGGC  >  NC_000913/3739117‑3739387

aTGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGt                                                                                                                                     >  2:1098870/1‑140 (MQ=255)

  ggATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTAc                                                                                                                                   >  1:1089824/1‑140 (MQ=255)

    aTACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACaa                                                                                                                                 <  1:1098870/140‑1 (MQ=255)

          ccTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCttt                                                                                                                           >  1:1059013/1‑140 (MQ=255)

          ccTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCttt                                                                                                                           >  1:286661/1‑140 (MQ=255)

          ccTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCttt                                                                                                                           >  1:584765/1‑140 (MQ=255)

           cTGGCAGCAAATGGGGGAAAAATTGCAGGGTTTCAACGTGTTGAGCTCCCTCTCGTCGCATGATACCCGCCTGTTCCGTGAA‑GGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTa                                                                                                                         <  1:275198/140‑1 (MQ=255)

               cagcaAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATgg                                                                                                                      >  2:410657/1‑140 (MQ=255)

                 gaaaaGGGCGGAAAAATTGCAGGTTTTCAACGTGTTGAGCTCCCTCTCGTCGCATGATCCCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGtg                                                                                                                    <  2:974116/138‑1 (MQ=255)

                    aaTGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTCCCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACaa                                                                                                                                 <  1:234723/124‑1 (MQ=255)

                    aaTGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACaa                                                                                                                                 >  2:234723/1‑124 (MQ=255)

                    aaTGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGtgatg                                                                                                                 >  1:894861/1‑140 (MQ=255)

                    aaTGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGtgatg                                                                                                                 >  1:729807/1‑140 (MQ=255)

                        gCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATc                                                                                                             >  2:616235/1‑140 (MQ=255)

                         cGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATcc                                                                                                            >  1:974116/1‑140 (MQ=255)

                         cGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATcc                                                                                                            >  1:46093/1‑140 (MQ=255)

                                    cAGGTTTTCAACGTGTTGAGCTCCCTCTCGTCGCATGATCCCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGtt                                                                                                 <  2:584765/140‑1 (MQ=255)

                                       ggTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCgg                                                                                              <  2:46093/140‑1 (MQ=255)

                                        gTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGt                                                                                             <  1:410657/140‑1 (MQ=255)

                                           tCAAGGTGTTGAGCTCCCTCTCGCCGCATGATCCCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCt                                                                                          <  2:894861/140‑1 (MQ=255)

                                           tCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCt                                                                                          >  1:470552/1‑140 (MQ=255)

                                           tCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCt                                                                                          <  2:470552/140‑1 (MQ=255)

                                           tCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCt                                                                                          >  1:253367/1‑140 (MQ=255)

                                           tCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCt                                                                                          >  1:633693/1‑140 (MQ=255)

                                           tCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCt                                                                                          >  1:906570/1‑140 (MQ=255)

                                              aggtgtTGAGCTCCCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACa                                                                                       <  1:308921/138‑1 (MQ=255)

                                                   ttGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTa                                                                                         <  1:1197325/133‑1 (MQ=255)

                                                   ttGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTa                                                                                         >  2:1197325/1‑133 (MQ=255)

                                                   ttGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTc                                                                                  >  1:674016/1‑140 (MQ=255)

                                                     gAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTg                                                                                <  2:286661/140‑1 (MQ=255)

                                                       gCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGAt                                                                              <  2:906570/140‑1 (MQ=255)

                                                           ccTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGc                                                                          >  1:464497/1‑140 (MQ=255)

                                                             tctcGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTg                                                                        >  1:223107/1‑140 (MQ=255)

                                                             tctcGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTg                                                                        <  2:871661/140‑1 (MQ=255)

                                                              ctcGCCGCATGATCCCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGc                                                                       <  2:970839/140‑1 (MQ=255)

                                                               tcgtcgCATGATCCCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCa                                                                      <  2:358380/140‑1 (MQ=255)

                                                               tcgtcgCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCa                                                                      <  2:1089824/140‑1 (MQ=255)

                                                               tcgtcgCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCa                                                                      <  2:674016/140‑1 (MQ=255)

                                                                cgtcgCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCaa                                                                     <  2:843646/140‑1 (MQ=255)

                                                                cgtcgCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCaa                                                                     <  2:253367/140‑1 (MQ=255)

                                                                    gCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTa                                                                 <  1:415353/140‑1 (MQ=255)

                                                                    gCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTa                                                                 <  2:698577/140‑1 (MQ=255)

                                                                      aTGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTaca                                                               >  2:685551/1‑140 (MQ=255)

                                                                         aTACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGt                                                            >  1:1178730/1‑140 (MQ=255)

                                                                          tACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGtt                                                           <  1:1175401/140‑1 (MQ=255)

                                                                           aCCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTc                                                          <  2:729807/140‑1 (MQ=255)

                                                                                  tGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATg                                                   <  1:967616/140‑1 (MQ=255)

                                                                                    ttCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATg                                                   >  2:948431/1‑138 (MQ=255)

                                                                                    ttCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATg                                                   <  1:948431/138‑1 (MQ=255)

                                                                                    ttCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGaa                                                 >  1:973302/1‑140 (MQ=255)

                                                                                    ttCCGGGATGAGAGCGAGAAAGCCGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGaa                                                 >  2:1195201/1‑140 (MQ=255)

                                                                                           aaGGGGGCGACAAGGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAg                                          >  2:490431/1‑140 (MQ=255)

                                                                                           aaGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAg                                          >  2:826449/1‑140 (MQ=255)

                                                                                            aGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAgg                                         >  2:746880/1‑140 (MQ=255)

                                                                                              ggggCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGAt                                       >  2:994490/1‑140 (MQ=255)

                                                                                                ggCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGt                                     >  1:188789/1‑140 (MQ=255)

                                                                                                ggCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGt                                     >  1:970432/1‑140 (MQ=255)

                                                                                                ggCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGt                                     >  1:248116/1‑140 (MQ=255)

                                                                                                ggCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTGCGGATATGAACTGGCAGGATGt                                     >  1:910652/1‑140 (MQ=255)

                                                                                                                          aTTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTcg           >  1:707757/1‑140 (MQ=255)

                                                                                                                          aTTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTcg           <  2:973302/140‑1 (MQ=255)

                                                                                                                          aTTAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTcg           >  1:1027023/1‑140 (MQ=255)

                                                                                                                            tAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTgccg                    >  2:1099138/1‑129 (MQ=255)

                                                                                                                            tAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTgccg                    <  1:1099138/129‑1 (MQ=255)

                                                                                                                            tAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCa                 <  2:202612/132‑1 (MQ=255)

                                                                                                                            tAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCa                 >  1:202612/1‑132 (MQ=255)

                                                                                                                            tAGCGCCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTcgcg         <  1:483632/140‑1 (MQ=255)

                                                                                                                              gcgcCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTCGCGCa       <  2:633693/140‑1 (MQ=255)

                                                                                                                                gcCAGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTCGCGCACt     <  1:1195201/140‑1 (MQ=255)

                                                                                                                                   aGGTGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTCGCGCACTGGc  >  2:263914/1‑140 (MQ=255)

ATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGGTTTCAACGTGTTGAGCTACCTCTCGTCGCATGATACCCGCCTGTTCCGTGAAGGGGGCGACAAAGCAGCAGAGTTATTACTATTAGCGCCAGGCGCGGTACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGTCCTACAGGTTCTGATCCGCTGCAAGGTACACGTTCGGATATGAACTGGCAGGATGTTAGCGGTAAATCTGCCGCCAGCGTCGCGCACTGGC  >  NC_000913/3739117‑3739387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A