Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,824,046	A→G	P549P (CCA→CCG)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,824,046	0	A	G	100.0%	278.3 / NA	75	P549P (CCA→CCG)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (35/40); total (35/40)

CGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATC  >  NC_000913/3823910‑3824181

cGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTccgccg                                                                                                                                      >  2:1106410/1‑140 (MQ=255)

   aGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCa                                                                                                                                   >  1:287644/1‑140 (MQ=255)

       aaaTATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAAccc                                                                                                                               <  1:1186981/140‑1 (MQ=255)

        aaTATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCa                                                                                                                              <  1:398632/140‑1 (MQ=255)

        aaTATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCa                                                                                                                              <  2:1023495/140‑1 (MQ=255)

          tatTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCaaa                                                                                                                            >  1:765893/1‑140 (MQ=255)

           atTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAg                                                                                                                           <  2:1128890/140‑1 (MQ=255)

                gcgcGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACg                                                                                                                      <  2:287644/140‑1 (MQ=255)

                 cgcgAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCa                                                                                                                              >  2:994927/1‑131 (MQ=255)

                 cgcgAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCa                                                                                                                              <  1:994927/131‑1 (MQ=255)

                 cgcgAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACgg                                                                                                                     >  2:130418/1‑140 (MQ=255)

                  gcgAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGa                                                                                                                    >  2:767980/1‑140 (MQ=255)

                    gAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACa                                                                                                                  >  2:1191043/1‑140 (MQ=255)

                    gAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACa                                                                                                                  >  1:302908/1‑140 (MQ=255)

                     aGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACAt                                                                                                                 <  2:302908/140‑1 (MQ=255)

                        tGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTg                                                                                                              <  2:1175184/140‑1 (MQ=255)

                        tGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTg                                                                                                              <  2:804420/140‑1 (MQ=255)

                        tGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGAACATCTg                                                                                                              <  2:798507/140‑1 (MQ=255)

                         ggATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGc                                                                                                             <  2:1040988/140‑1 (MQ=255)

                           aTCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGccc                                                                                                           <  1:130418/140‑1 (MQ=255)

                               cATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTa                                                                                                       >  2:430761/1‑140 (MQ=255)

                                aTGAAGCTGGCAACGCTTGACAATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTaa                                                                                                      <  1:173169/140‑1 (MQ=255)

                                   aaGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAgg                                                                                                   <  1:1226330/140‑1 (MQ=255)

                                    aGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGt                                                                                                  >  2:859399/1‑140 (MQ=255)

                                         gCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGa                                                                                             >  1:1123309/1‑140 (MQ=255)

                                          cAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGAt                                                                                            >  2:100912/1‑140 (MQ=255)

                                          cAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGAt                                                                                            >  1:887452/1‑140 (MQ=255)

                                             cGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGc                                                                                         >  1:836111/1‑140 (MQ=255)

                                              gCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCg                                                                                        <  2:288032/140‑1 (MQ=255)

                                              gCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCg                                                                                        >  1:184933/1‑140 (MQ=255)

                                                ttGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAg                                                                                                    <  1:734081/126‑1 (MQ=255)

                                                ttGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAg                                                                                                    >  2:734081/1‑126 (MQ=255)

                                                       tCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCa                                                                               >  2:454112/1‑140 (MQ=255)

                                                        ctgctgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCAc                                                                              >  1:487211/1‑140 (MQ=255)

                                                         tgctgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCAcc                                                                             >  1:198696/1‑140 (MQ=255)

                                                          gctgGCAGAAATCGGGCTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGGGCCGAGGGCGGGCTGATCACCt                                                                            >  2:193995/1‑140 (MQ=255)

                                                          gctgGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCt                                                                            >  1:749768/1‑140 (MQ=255)

                                                                        gACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAAtgctgc                                                              <  2:749768/140‑1 (MQ=255)

                                                                            tGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTgccgcc                                                          <  2:1005847/140‑1 (MQ=255)

                                                                                   gCAATGAGTGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTcc                                                   >  1:19537/1‑140 (MQ=255)

                                                                                       tGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGc                                               >  1:1094630/1‑140 (MQ=255)

                                                                                       tGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGc                                               <  1:430761/140‑1 (MQ=255)

                                                                                        gAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGccc                                                         >  1:177592/1‑129 (MQ=255)

                                                                                        gAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGccc                                                         <  2:177592/129‑1 (MQ=255)

                                                                                        gAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCg                                              >  1:627853/1‑140 (MQ=255)

                                                                                           cGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGAcc                                           <  1:736248/140‑1 (MQ=255)

                                                                                              ggtggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGa                                        <  2:505850/140‑1 (MQ=255)

                                                                                                 ggtCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTa                                     <  1:1191043/140‑1 (MQ=255)

                                                                                                    cgcgAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCg                                  >  2:815642/1‑140 (MQ=255)

                                                                                                      cgAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGcc                                <  1:35068/140‑1 (MQ=255)

                                                                                                      cgAAAAATCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGcc                                <  2:487211/140‑1 (MQ=255)

                                                                                                            aTCTGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTc                          >  2:635955/1‑140 (MQ=255)

                                                                                                               tGCAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGc                       <  2:627853/140‑1 (MQ=255)

                                                                                                                 cAACATGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGccc                     <  2:19537/140‑1 (MQ=255)

                                                                                                                     aTGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGt                 >  2:415088/1‑140 (MQ=255)

                                                                                                                      tGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTa                >  2:533878/1‑140 (MQ=255)

                                                                                                                      tGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTa                <  1:193995/140‑1 (MQ=255)

                                                                                                                      tGGGGACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTa                >  2:878156/1‑140 (MQ=255)

                                                                                                                       ggggACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTaa               <  2:184933/140‑1 (MQ=255)

                                                                                                                       ggggACGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTaa               <  2:836111/140‑1 (MQ=255)

                                                                                                                            cGCCTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTc          <  1:815642/140‑1 (MQ=255)

                                                                                                                              ccTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTg        >  2:397866/1‑140 (MQ=255)

                                                                                                                              ccTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTg        <  2:198696/140‑1 (MQ=255)

                                                                                                                              ccTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTg        >  2:501263/1‑140 (MQ=255)

                                                                                                                              ccTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTg        >  2:812335/1‑140 (MQ=255)

                                                                                                                               cTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGccc                     >  2:455122/1‑126 (MQ=255)

                                                                                                                               cTCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGccc                     <  1:455122/126‑1 (MQ=255)

                                                                                                                               cTCCATTCCGCCGGCAACCCAAAGCCACGGAC‑TCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGt      >  2:1273281/1‑140 (MQ=255)

                                                                                                                                tCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGt      >  1:470911/1‑140 (MQ=255)

                                                                                                                                tCCATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGt      <  1:397866/140‑1 (MQ=255)

                                                                                                                                  cATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTaa               >  2:1034590/1‑129 (MQ=255)

                                                                                                                                  cATTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTaa               <  1:1034590/129‑1 (MQ=255)

                                                                                                                                   aTTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGAt   <  1:594348/140‑1 (MQ=255)

                                                                                                                                   aTTCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGAt   <  2:466620/140‑1 (MQ=255)

                                                                                                                                    ttCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATc  <  1:859399/140‑1 (MQ=255)

                                                                                                                                    ttCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATc  <  1:533878/140‑1 (MQ=255)

                                                                                                                                    ttCCGCCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATc  <  2:887452/140‑1 (MQ=255)

CGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAAGCTGGCAACGCTTGACGATCTGCTGGCAGAAATCGGACTTGGTAACGCAATGAGCGTGGTGGTCGCGAAAAATCTGCAACATGGGGACGCCTCCATTCCACCGGCAACCCAAAGCCACGGACATCTGCCCATTAAAGGTGCCGATGGCGTGCTGATCACCTTTGCGAAATGCTGCCGCCCTATTCCTGGCGACCCGATTATCGCCCACGTCAGCCCCGGTAAAGGTCTGGTGATC  >  NC_000913/3823910‑3824181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A