Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,882,325	T→C	intergenic (‑4/+1)	dnaN ← / ← dnaA	beta sliding clamp/chromosomal replication initiator protein DnaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,882,325	0	T	C	100.0%	274.6 / NA	75	intergenic (‑4/+1)	dnaN/dnaA	beta sliding clamp/chromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (40/35); total (40/35)

GTCAGCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCT  >  NC_000913/3882188‑3882459

gTCAGCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGctt                                                                                                                                      >  2:206098/1‑140 (MQ=255)

gTCAGCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGctt                                                                                                                                      >  1:254959/1‑140 (MQ=255)

gTCAGCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGctt                                                                                                                                      >  1:884836/1‑140 (MQ=255)

    gCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGa                                                                                                                                  <  2:1135903/140‑1 (MQ=255)

    gCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGa                                                                                                                                  <  1:471773/140‑1 (MQ=255)

    gCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGa                                                                                                                                  <  2:521179/140‑1 (MQ=255)

                  tCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGa                                                                                                                    >  1:569035/1‑140 (MQ=255)

                       aaCCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTaaa                                                                                                               >  2:293163/1‑140 (MQ=255)

                        aCCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAAt                                                                                                              >  2:1161924/1‑140 (MQ=255)

                            gTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGa                                                                                                          >  2:28/1‑140 (MQ=255)

                             tAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGaa                                                                                                         <  2:884836/140‑1 (MQ=255)

                                   cagATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAAtct                                                                                                   >  2:538407/1‑140 (MQ=255)

                                   cagATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAAtct                                                                                                   >  2:1262501/1‑140 (MQ=255)

                                    agATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAAtctt                                                                                                  >  1:83569/1‑140 (MQ=255)

                                     gATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAAtcttc                                                                                                 >  1:1245929/1‑140 (MQ=255)

                                     gATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAAtcttc                                                                                                 >  2:162243/1‑140 (MQ=255)

                                       ttACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATcttctt                                                                                               >  1:755988/1‑140 (MQ=255)

                                             agaATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGatat                                                                                         >  1:858122/1‑140 (MQ=255)

                                                aTCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGt                                                                                      >  1:1057221/1‑140 (MQ=255)

                                                   ggTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGc                                                                                   <  1:264814/140‑1 (MQ=255)

                                                    gTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGct                                                                                  >  1:262964/1‑140 (MQ=255)

                                                    gTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGct                                                                                  <  1:162243/140‑1 (MQ=255)

                                                    gTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGct                                                                                  <  1:1129287/140‑1 (MQ=255)

                                                      aGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGctct                                                                                <  1:293163/140‑1 (MQ=255)

                                                         gTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCtt                                                                             >  2:976105/1‑140 (MQ=255)

                                                              acgacCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGc                                                                        <  2:858122/140‑1 (MQ=255)

                                                                 accaccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGct                                                                                  >  2:207864/1‑127 (MQ=255)

                                                                 accaccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGct                                                                                  <  1:207864/127‑1 (MQ=255)

                                                                 accaccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAAc                                                                     >  1:748144/1‑140 (MQ=255)

                                                                  ccaccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCa                                                                           >  1:984182/1‑133 (MQ=255)

                                                                  ccaccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCa                                                                           <  2:984182/133‑1 (MQ=255)

                                                                  ccaccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGT‑AATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGc                                                                        <  2:585897/135‑1 (MQ=255)

                                                                  ccaccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGT‑AATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGc                                                                        >  1:585897/1‑135 (MQ=255)

                                                                     ccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGGCAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 >  1:1096181/1‑140 (MQ=255)

                                                                     ccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 <  1:355908/140‑1 (MQ=255)

                                                                     ccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 <  2:1245929/140‑1 (MQ=255)

                                                                     ccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 <  2:1057221/140‑1 (MQ=255)

                                                                     ccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 <  1:206098/140‑1 (MQ=255)

                                                                     ccTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 <  1:976105/140‑1 (MQ=255)

                                                                      cTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTc                                                                <  2:748144/140‑1 (MQ=255)

                                                                        aaCGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGa                                                              >  2:125135/1‑140 (MQ=255)

                                                                               cGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 >  1:910629/1‑130 (MQ=255)

                                                                               cGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCt                                                                 <  2:910629/130‑1 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCa                                                                       <  1:378765/123‑1 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCa                                                                       >  2:378765/1‑123 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGa                                                              <  2:304335/132‑1 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGa                                                              >  1:304335/1‑132 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACgg                                                      >  1:835616/1‑140 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACgg                                                      >  1:1018693/1‑140 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACgg                                                      >  2:1193558/1‑140 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACgg                                                      >  1:976620/1‑140 (MQ=255)

                                                                                gCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACgg                                                      >  1:973015/1‑140 (MQ=255)

                                                                                      cTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGGCAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACggcaggca                                                <  2:1096181/140‑1 (MQ=255)

                                                                                      cTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACggcaggca                                                >  2:978758/1‑140 (MQ=255)

                                                                                        gttgtAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATg                                              <  2:254959/140‑1 (MQ=255)

                                                                                             aGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCa                                         <  1:1262501/140‑1 (MQ=255)

                                                                                                ggTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACcg                                      <  1:284768/140‑1 (MQ=255)

                                                                                                ggTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACcg                                      <  2:569035/140‑1 (MQ=255)

                                                                                                ggTTTTAATAAATGCTCACGTTCTACGGTAAATTACATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAg                                           >  2:860621/1‑135 (MQ=255)

                                                                                                ggTTTTAATAAATGCTCACGTTCTACGGTAAATTACATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAg                                           <  1:860621/135‑1 (MQ=255)

                                                                                                    ttAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACcgtcgt                                  <  2:835616/140‑1 (MQ=255)

                                                                                                    ttAATAAATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTATGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTCACGGCAGGCATGAAGCACcgtcgt                                  <  1:711234/140‑1 (MQ=255)

                                                                                                     taataaATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCgtg                                 <  1:1161924/140‑1 (MQ=255)

                                                                                                     taataaATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCgtg                                 <  2:262964/140‑1 (MQ=255)

                                                                                                      aataaATGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCgtgt                                >  2:1150050/1‑140 (MQ=255)

                                                                                                            tGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCAc                          >  2:308478/1‑140 (MQ=255)

                                                                                                            tGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCAc                          >  1:322200/1‑140 (MQ=255)

                                                                                                            tGCTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCAc                          >  1:859764/1‑140 (MQ=255)

                                                                                                              cTCACGTTCTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAACTCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACgg                        >  2:584123/1‑140 (MQ=255)

                                                                                                                      cTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCgtg                                 <  1:187216/123‑1 (MQ=255)

                                                                                                                      cTACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCgtg                                 >  2:187216/1‑123 (MQ=255)

                                                                                                                       tACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAAc               >  1:702259/1‑140 (MQ=255)

                                                                                                                       tACGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCATACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAAc               >  1:115339/1‑140 (MQ=255)

                                                                                                                        aCGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACg              <  2:973015/140‑1 (MQ=255)

                                                                                                                         cGGTAAATTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGc             >  1:1166916/1‑140 (MQ=255)

                                                                                                                              aaTTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGc        <  2:859764/140‑1 (MQ=255)

                                                                                                                               aTTTCATAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGcc       >  2:811615/1‑140 (MQ=255)

                                                                                                                                    aTAGGCTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAAtct  <  2:322200/140‑1 (MQ=255)

GTCAGCGACAACGTACCGTCAGCAACCTGTAACAGCAGATTACCGAGAATCGGTAGCGTAGGACGACCACCTAACGGACCGCTCACCTGTTGTAGCGGTTTTAATAAATGCTCACGTTCTACGGTAAATTTCATAGGTTTACGATGACAATGTTCTGATTAAATTTGAAAAATCTTCTTTGATATCGTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCT  >  NC_000913/3882188‑3882459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A