Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 161 75.5 2581324 99.3% 2563254 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,965,013 A→G R206R (CGT→CGC)  rhlB ← ATP‑dependent RNA helicase RhlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,965,0130AG100.0% 411.4 / NA 110R206R (CGT→CGC) rhlBATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/59);  total (51/59)

Only 100 of 111 total aligned reads displayed.

TCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACC  >  NC_000913/3964880‑3965145
                                                                                                                                     |                                                                                                                                    
tCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTAcc                                                                                                                                >  1:855249/1‑140 (MQ=255)
tCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTAcc                                                                                                                                <  1:1029857/140‑1 (MQ=255)
tCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTAcc                                                                                                                                <  2:1195736/140‑1 (MQ=255)
tCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTAcc                                                                                                                                <  1:365575/140‑1 (MQ=255)
    cGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGt                                                                                                                            >  2:421637/1‑140 (MQ=255)
         gcagcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGaa                                                                                                                       >  2:79978/1‑140 (MQ=255)
             caAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCg                                                                                                                   <  1:88505/140‑1 (MQ=255)
              aaaCGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGt                                                                                                                  >  2:1155909/1‑140 (MQ=255)
                  gCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCg                                                                                                              <  1:126198/140‑1 (MQ=255)
                     ttttttCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGgag                                                                                                           <  2:855249/140‑1 (MQ=255)
                     ttttttCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGgag                                                                                                           <  1:509590/140‑1 (MQ=255)
                                        gTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCt                                                                                        <  2:1157885/140‑1 (MQ=255)
                                           gAAACGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACa                                                                                                       <  1:912339/122‑1 (MQ=255)
                                           gAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACa                                                                                                       >  2:912339/1‑122 (MQ=255)
                                               aGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGc                                                                                 <  1:668800/140‑1 (MQ=255)
                                                gCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCa                                                                                <  1:637906/140‑1 (MQ=255)
                                                 ctctTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAg                                                                               >  1:1042603/1‑140 (MQ=255)
                                                  tcttctTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAgg                                                                              >  2:675626/1‑140 (MQ=255)
                                                  tcttctTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAgg                                                                              <  2:656206/140‑1 (MQ=255)
                                                  tcttctTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAgg                                                                              <  2:31546/140‑1 (MQ=255)
                                                              cGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATAcggcg                                                                  <  1:827453/140‑1 (MQ=255)
                                                                gTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGa                                                                >  2:339970/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGTGGaa                                                               >  1:1215054/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  2:59650/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  1:1010772/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  1:636090/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  2:537524/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  1:854792/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  1:895845/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  2:1241125/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  1:1271454/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  1:310524/1‑140 (MQ=255)
                                                                 tGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGaa                                                               >  1:408734/1‑140 (MQ=255)
                                                                   gCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAca                                                             >  2:1100648/1‑140 (MQ=255)
                                                                     ccGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagc                                                           <  2:1271454/140‑1 (MQ=255)
                                                                     ccGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagc                                                           <  1:982823/140‑1 (MQ=255)
                                                                      cGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagcc                                                          >  1:570958/1‑140 (MQ=255)
                                                                        ttttCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAAcagccag                                                        >  2:168353/1‑140 (MQ=255)
                                                                           tCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCgg                                                     <  2:408734/140‑1 (MQ=255)
                                                                           tCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCgg                                                     <  2:310524/140‑1 (MQ=255)
                                                                           tCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCgg                                                     <  1:675626/140‑1 (MQ=255)
                                                                           tCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGCACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCgg                                                     <  2:895845/140‑1 (MQ=255)
                                                                            cTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGa                                                    <  1:537524/140‑1 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   >  1:825623/1‑140 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   <  2:1286140/140‑1 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   <  2:825623/140‑1 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   >  1:859640/1‑140 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   <  2:859640/140‑1 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   <  1:168353/140‑1 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   >  1:1178183/1‑140 (MQ=255)
                                                                             tGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGat                                                   <  2:570958/140‑1 (MQ=255)
                                                                                tCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATc                                                <  1:240809/140‑1 (MQ=255)
                                                                                      tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaat                                          <  1:1241125/140‑1 (MQ=255)
                                                                                      tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaat                                          <  1:1100648/140‑1 (MQ=255)
                                                                                      tCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGATTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTtaat                                          <  2:228023/140‑1 (MQ=255)
                                                                                         aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaa                                       >  2:1007531/1‑140 (MQ=255)
                                                                                         aCTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATaaa                                       >  1:806828/1‑140 (MQ=255)
                                                                                           ttCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGTGGAACAGCCAGCGGATATCTTTAATAAAGc                                     <  2:1215054/140‑1 (MQ=255)
                                                                                            tCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                                    <  1:824136/140‑1 (MQ=255)
                                                                                              aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                                  <  2:1048242/140‑1 (MQ=255)
                                                                                              aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                                  <  1:421637/140‑1 (MQ=255)
                                                                                              aaTATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCa                                  <  2:597071/140‑1 (MQ=255)
                                                                                               atatatTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAg                                 >  2:620991/1‑140 (MQ=255)
                                                                                                   atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                              <  2:1003743/139‑1 (MQ=255)
                                                                                                   atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATc                              >  1:1003743/1‑139 (MQ=255)
                                                                                                   atTCGGCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCg                             >  2:1099768/1‑140 (MQ=255)
                                                                                                       ggCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACa                         >  1:610289/1‑140 (MQ=255)
                                                                                                       ggCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACa                         <  1:809311/140‑1 (MQ=255)
                                                                                                       ggCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACa                         >  2:131736/1‑140 (MQ=255)
                                                                                                        gCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                                    >  2:971065/1‑128 (MQ=255)
                                                                                                        gCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                                    <  1:971065/128‑1 (MQ=255)
                                                                                                        gCATTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACAt                        >  2:1193574/1‑140 (MQ=255)
                                                                                                          aTTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGGTATCTTTAATAAAGCCCAGATCGTACATGc                      >  2:952382/1‑140 (MQ=255)
                                                                                                          aTTGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGc                      >  1:42259/1‑140 (MQ=255)
                                                                                                            tGTTCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGa                    >  2:774719/1‑140 (MQ=255)
                                                                                                              ttCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                                    >  2:282760/1‑122 (MQ=255)
                                                                                                              ttCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGcc                                    <  1:282760/122‑1 (MQ=255)
                                                                                                              ttCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATc                  >  2:479627/1‑140 (MQ=255)
                                                                                                              ttCATCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATc                  >  1:289/1‑140 (MQ=255)
                                                                                                                  tCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt              <  2:1042603/140‑1 (MQ=255)
                                                                                                                  tCTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCt              <  2:42259/140‑1 (MQ=255)
                                                                                                                   cTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCtt             >  2:220357/1‑140 (MQ=255)
                                                                                                                   cTGCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCtt             <  1:126842/140‑1 (MQ=255)
                                                                                                                     gCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg           <  1:1099768/140‑1 (MQ=255)
                                                                                                                     gCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg           <  2:854792/140‑1 (MQ=255)
                                                                                                                     gCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg           <  1:534870/140‑1 (MQ=255)
                                                                                                                     gCTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCg           <  1:952382/140‑1 (MQ=255)
                                                                                                                      cTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGt          >  2:649766/1‑140 (MQ=255)
                                                                                                                      cTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGt          >  1:760200/1‑140 (MQ=255)
                                                                                                                      cTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGt          >  1:817527/1‑140 (MQ=255)
                                                                                                                      cTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGt          <  1:220357/140‑1 (MQ=255)
                                                                                                                      cTCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGt          <  1:194079/140‑1 (MQ=255)
                                                                                                                       tCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGAt                   >  1:91668/1‑130 (MQ=255)
                                                                                                                       tCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGAt                   <  2:91668/130‑1 (MQ=255)
                                                                                                                       tCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTc         >  1:534799/1‑140 (MQ=255)
                                                                                                                       tCGAACGCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTc         <  2:1084949/140‑1 (MQ=255)
                                                                                                                             gCCAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTac   <  1:774719/140‑1 (MQ=255)
                                                                                                                              ccAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTacc  <  1:59650/140‑1 (MQ=255)
                                                                                                                              ccAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTacc  <  2:562586/140‑1 (MQ=255)
                                                                                                                              ccAGTTCGCGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTacc  <  1:1155909/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCATACGGCGGAACAGCCAGCGGATATCTTTAATAAAGCCCAGATCGTACATGCGATCGGCTTCGTCCAGTACC  >  NC_000913/3964880‑3965145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A