Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,200,176	(G)_6→7	intergenic (+82/‑105)	yjaG → / → hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,200,170	1	.	G	98.6%	263.2 / ‑3.3	72	intergenic (+76/‑111)	yjaG/hupA	conserved protein YjaG/DNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (35/36); total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

TTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑GG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGAT  >  NC_000913/4200036‑4200300

ttAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑gg‑ggg                                                                                                                               >  1:21773/1‑140 (MQ=255)

  aGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑gg‑ggggg                                                                                                                             >  2:1203538/1‑139 (MQ=255)

  aGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑gg‑ggggg                                                                                                                             <  2:1088713/140‑2 (MQ=255)

  aGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑gg‑ggggg                                                                                                                             <  1:258891/140‑2 (MQ=255)

    ggCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑gg‑gggggcg                                                                                                                           >  1:95600/1‑137 (MQ=255)

        gACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGaaaa                                                                                                                        >  2:146192/1‑140 (MQ=255)

             gCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGt                                                                                                                   <  1:476963/140‑1 (MQ=255)

             gCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGt                                                                                                                   <  2:911601/140‑1 (MQ=255)

              cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTg                                                                                                                  >  2:934790/1‑140 (MQ=255)

              cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTg                                                                                                                  >  2:163048/1‑140 (MQ=255)

              cGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAAT‑AGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTgg                                                                                                                 <  2:850164/140‑1 (MQ=255)

                   ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTAt                                                                                                             <  1:531085/140‑1 (MQ=255)

                   ggCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTAt                                                                                                             <  2:7608/140‑1 (MQ=255)

                    gCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑GGAGGGGCGAAAAAAAGTGGCTATc                                                                                                            <  2:534026/140‑1 (MQ=255)

                       ggTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGt                                                                                                         >  2:28347/1‑140 (MQ=255)

                        gTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTg                                                                                                        >  2:1170380/1‑140 (MQ=255)

                        gTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTg                                                                                                        >  1:1253373/1‑140 (MQ=255)

                        gTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTg                                                                                                        >  2:189763/1‑140 (MQ=255)

                         tGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGc                                                                                                       <  1:569494/140‑1 (MQ=255)

                         tGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGc                                                                                                       <  1:810093/140‑1 (MQ=255)

                           agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     <  1:291431/140‑1 (MQ=255)

                           agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     <  1:189763/140‑1 (MQ=255)

                           agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     <  1:1203538/140‑1 (MQ=255)

                           agagCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     <  2:21773/140‑1 (MQ=255)

                                  tatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCa                                                                                              >  2:1261579/1‑140 (MQ=255)

                                  tatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCa                                                                                              >  1:811954/1‑140 (MQ=255)

                                  tatTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCa                                                                                              <  1:1170380/140‑1 (MQ=255)

                                     tGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     <  2:545092/130‑1 (MQ=255)

                                     tGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     >  1:545092/1‑130 (MQ=255)

                                       gTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     >  2:789056/1‑128 (MQ=255)

                                       gTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCgt                                                                                                     <  1:789056/128‑1 (MQ=255)

                                       gTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCAGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGgaga                                                                                         >  1:821388/1‑140 (MQ=255)

                                          taATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTg                                                                                      >  2:825669/1‑140 (MQ=255)

                                            aTTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCt                                                                                    >  2:72058/1‑140 (MQ=255)

                                            aTTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCt                                                                                    >  2:698237/1‑140 (MQ=255)

                                                   aGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTgg                                                                             <  1:1058746/140‑1 (MQ=255)

                                                            aCCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGt                                                                    <  2:198725/140‑1 (MQ=255)

                                                                     cGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGc                                                                 >  2:1017448/1‑134 (MQ=255)

                                                                     cGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGc                                                                 <  1:1017448/134‑1 (MQ=255)

                                                                     cGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGa                                                           >  1:897827/1‑140 (MQ=255)

                                                                     cGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGa                                                           >  2:146335/1‑140 (MQ=255)

                                                                      gTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGAt                                                          <  1:934790/140‑1 (MQ=255)

                                                                        gATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGc                                                        >  1:227755/1‑140 (MQ=255)

                                                                             aaCGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGc                                                   <  1:28347/140‑1 (MQ=255)

                                                                                 ccTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGc                                               <  2:95600/140‑1 (MQ=255)

                                                                                    gatatGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGAt                                            >  2:733359/5‑140 (MQ=255)

                                                                                    gATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGAt                                            >  1:1015213/1‑140 (MQ=255)

                                                                                     aTTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATa                                           >  1:991588/1‑140 (MQ=255)

                                                                                         gTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       <  2:124577/140‑1 (MQ=255)

                                                                                         gTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       <  1:72058/140‑1 (MQ=255)

                                                                                         gTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       <  2:1015213/140‑1 (MQ=255)

                                                                                         gTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGACATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       <  2:438694/140‑1 (MQ=255)

                                                                                         gTCGTACCTGGAGTCTTCCCTTTCGCCCCCAGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       <  2:821388/140‑1 (MQ=255)

                                                                                                     gTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt                           <  1:103710/140‑1 (MQ=255)

                                                                                                     gTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt                           <  2:1253373/140‑1 (MQ=255)

                                                                                                     gTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt                           <  1:698237/140‑1 (MQ=255)

                                                                                                      tCTTCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATa                          >  2:103848/1‑140 (MQ=255)

                                                                                                        ttCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       >  2:30554/1‑125 (MQ=255)

                                                                                                        ttCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       <  1:30554/125‑1 (MQ=255)

                                                                                                        ttCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc                        <  1:825669/140‑1 (MQ=255)

                                                                                                        ttCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc                        >  1:16643/1‑140 (MQ=255)

                                                                                                        ttCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc                        >  2:430352/1‑140 (MQ=255)

                                                                                                        ttCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAAc                        >  1:62676/1‑140 (MQ=255)

                                                                                                         tCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       >  2:959164/1‑124 (MQ=255)

                                                                                                         tCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAaca                                       <  1:959164/124‑1 (MQ=255)

                                                                                                         tCCCTTTCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACt                       >  1:774490/1‑140 (MQ=255)

                                                                                                              ttCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGa                  >  2:486500/1‑140 (MQ=255)

                                                                                                               tCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGaa                 <  1:254731/140‑1 (MQ=255)

                                                                                                               tCGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGaa                 <  1:1261579/140‑1 (MQ=255)

                                                                                                                cGCCCCCCGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAAc                >  2:243863/1‑140 (MQ=255)

                                                                                                                  ccccccGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACaa              >  1:532157/1‑140 (MQ=255)

                                                                                                                   cccccGTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAg             <  2:532157/140‑1 (MQ=255)

                                                                                                                        gTCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCa        <  2:16643/140‑1 (MQ=255)

                                                                                                                         tCTGGTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCaa       >  1:1037461/1‑140 (MQ=255)

                                                                                                                             gTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACtga   >  2:588819/1‑140 (MQ=255)

                                                                                                                             gTCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACtga   >  1:543845/1‑140 (MQ=255)

                                                                                                                              tCTACATTTGGG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACtgat  <  1:650191/140‑1 (MQ=255)

TTAGGGCAGACCTGCGTGAGGCGGGTGAGAGCAATATTGGTATAATTTTTCAGCAATAAGACCAGAAAACGTGATTTAACGCCTGATTTGTCGTACCTGGAGTCTTCCCTTTCGCCCCCCGTCTGGTCTACATTT‑GG‑GGGGCGAAAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGAT  >  NC_000913/4200036‑4200300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A