Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,204,349	+C	coding (1030/1326 nt)	zraR →	DNA‑binding transcriptional activator ZraR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,204,345	1	.	C	100.0%	156.0 / NA	43	T342T (ACG→ACC)	zraR	DNA‑binding transcriptional activator ZraR
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (13/30); total (13/30)

ATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACG‑CCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGC  >  NC_000913/4204209‑4204479

aTCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACG‑ccc                                                                                                                                      <  1:62945/140‑1 (MQ=255)

 tctcTACTATCGCCTGATTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACG‑cccc                                                                                                                                     <  2:1146767/140‑1 (MQ=255)

 tctcTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACG‑cccc                                                                                                                                     <  2:550226/140‑1 (MQ=255)

      actaTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACG‑cccccaggc                                                                                                                                <  1:134185/140‑6 (MQ=255)

        taTCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGa                                                                                                                               <  1:615318/140‑1 (MQ=255)

            gCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGa                                                                                                                           <  1:61292/140‑1 (MQ=255)

            gCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGa                                                                                                                           <  1:468437/140‑1 (MQ=255)

             gacagaTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGAt                                                                                                                          <  1:649152/135‑1 (MQ=255)

             ccTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGCAGATATCCCTCTTCCCGCTGGCCCATTTTTTCGGCGC‑CTTGCCGGGGGGCAT‑CCC‑AAAGCGGGGAAAA‑GGTTTTTGC‑CCCCCGCCGATggga                                                                                                                          >  2:1193542/1‑138 (MQ=255)

             ccTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCCCTGCTGGGCGGCCCCTTTCTCGAGCGC‑GTTTGCGAACGGAAT‑CGC‑AAAGCGGGAAAAA‑GGTTTTCAC‑CCCCCGGCgcggggg                                                                                                                          >  2:568128/1‑134 (MQ=255)

                 aaTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGGAGAAATTCCTCTTCTGGGTGGGCCTTTTTTTCCGCGCTTTTGCCG‑GCGGAAA‑CGC‑AAAGCGGGGAAAA‑GGTTTTAGC‑CCCCCCGCCGTGGGTCTGt                                                                                                                      >  2:615318/1‑140 (MQ=255)

                 aaTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGGAGAAATTCCTCTGCTGGCTGGGCCTTTTTTTCAGCGC‑CTTTCCGAGCGGAAT‑CGC‑AAAGCGGGGAAAA‑GGTTTTCGC‑CCCCCGGCGGTGGGTCTGt                                                                                                                      >  2:408864/1‑140 (MQ=255)

                 aaTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATTCCCCTGCTGGCTGGGCATTTTTTGCAGCGC‑TTTTGCGAGCGGAAT‑CGC‑AAAGC‑CGGAAAA‑GGGTTTTCGCCCCCCCGCGATGGGTCTGt                                                                                                                      >  2:57806/1‑140 (MQ=255)

                 aaTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCCTTTTCTGCCGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGCGGGAAAAA‑GGTTTTCGC‑CCCCCGGCGGTGGGTCTGt                                                                                                                      >  2:979808/1‑140 (MQ=255)

                 aaTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGTGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGt                                                                                                                      <  1:423078/140‑1 (MQ=255)

                 aaTGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCCCTGCTGGCTGGGCCTTTTTTTCAGCGC‑TTTGCCCGGCGGAAT‑CGC‑AAGGCGGGAAAAA‑GGTTTTCGC‑CCCCCGGCGATGGGGCTGt                                                                                                                      >  2:680749/1‑140 (MQ=255)

                    gtggtgGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGa                                                                                                                   <  1:97240/140‑1 (MQ=255)

                    gtggtgGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGa                                                                                                                   <  2:1043901/140‑1 (MQ=255)

                    gtggtgGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGa                                                                                                                   <  1:1221952/140‑1 (MQ=255)

                    gtggtgGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGa                                                                                                                   <  1:171963/140‑1 (MQ=255)

                    gtggtgGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGa                                                                                                                   <  1:408864/140‑1 (MQ=255)

                        tggCGATTGAAGTACCATCGCTGCGGCAACGGCGGGGAGATATCCCTCTTCCGGCTGGCCCTTTTTTTCCGCGC‑CTTTTCCCGCGGAATCCCC‑AAGCA‑GGGAAAG‑GGTTTTCGC‑CCCCCCGCGGTGGGTCTTTTGATTct                                                                                                               >  2:1221952/1‑139 (MQ=255)

                           cGATTGAAGTACCATCGCTGCGGCAACGGCGGGGAGATATCCCTCTTCTTGCTGGGCATTTTTTTCCGCGC‑TTTGCCGAGGGGAAT‑AGC‑AAGGCGGGAAAAA‑GGTTTTTGT‑CCCCCGCCGATGGGTTTGTTGGttttttt                                                                                                            >  2:171963/1‑135 (MQ=255)

                             aTTGAAGTACCATCGCTGCGGCAACGGCGGGGAGAAATTCCCCTGCTGGCTGGCCATTTTTTTCAGCGC‑TTTGGCGCGCGGAAT‑CGC‑AAGGC‑GGGAAAAGGGTTTTTGC‑CCCCCGGCGGTGGGTCTGTTGttttactgtc                                                                                                          >  2:649152/1‑133 (MQ=255)

                                   gTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGc                                                                                                   <  1:769886/140‑1 (MQ=255)

                                     aCCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCg                                                                                                 <  1:568128/140‑1 (MQ=255)

                                     aCCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCg                                                                                                 <  1:806975/140‑1 (MQ=255)

                                      ccATCGCTGCGGCAACGGCGGGAAGATATTCCCCTGCTGGCTGGCCCTTTTTTGCAGCGC‑TTTGCCGAGCGGAAT‑CGC‑AAAGCGGGAAAAA‑GGTTTTCGC‑CCCCCCGCGATGGGTCTGTTGATTCATTACGA‑CTGGCCgg                                                                                                >  2:334013/1‑140 (MQ=255)

                                       cATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCggg                                                                                               <  1:679005/140‑1 (MQ=255)

                                         tCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGaa                                                                                             <  1:207193/140‑1 (MQ=255)

                                            cTGCGGCAACGGCGGGAAGAAATTCCTCTGCTGGCTGGGCCTTTTTTTCCGCGC‑TTTTCCGGGCGGAAA‑CGCAAAGGG‑GGAAAAA‑GGTTTTTTC‑CCCCCCGCGGTGGGTCTGTTTGTTTATTTAGA‑CTGGCCGGGgaaaa                                                                                          >  2:324345/1‑138 (MQ=255)

                                             tGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAatat                                                                                         <  1:430184/140‑1 (MQ=255)

                                              gCGGCAACGGCGGGAAGATATTCCTCTGCTGGCTGGCCATTTTTTTCCGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAAGCGGGAAAAA‑GGTTTTAGC‑CCCCCCGCGATGGATCTGTTGATTTATTACGACCTGCCCGGGaaaatt                                                                                         >  2:1020547/1‑137 (MQ=255)

                                               cGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTc                                                                                       <  1:1155906/140‑1 (MQ=255)

                                               cGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTc                                                                                       <  1:333496/140‑1 (MQ=255)

                                               cGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTc                                                                                       <  2:977701/140‑1 (MQ=255)

                                                    aCGGCGGGAAGATATCCCCCTGCTGGCTGGGCCTTTTTTGCAGCGC‑CTTTCCGCGCGGAAT‑CGC‑AAAGC‑GGGAAAA‑GGGTTTTCGCCCCCCCGCGGTGGGTCTGTTGATTTATTACGA‑CTGGCCGGGGAAAATTCTTGAg                                                                                  >  2:50299/1‑140 (MQ=255)

                                                     cggcggGAAGATATCCCTCTGCTGGCTGGCCATTTTCTTCAGCGC‑TTTGCCGAGCGGAAT‑CGC‑AAGGCGGGAAAAA‑GGTTTTCGC‑CCCCCCGCGGTGGGTCTGTTGATTTATTTCGA‑CTGGCCGGGGAAAATTTGTGAgg                                                                                 >  2:333496/1‑139 (MQ=255)

                                                            aaGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGaaaa                                                                          <  1:1193542/140‑1 (MQ=255)

                                                                tatCCCTCTGCTGGCTGGCCATTTTTTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAAGC‑GGTAAAA‑GGGTTTACGCCCCCAGGCGATGGGTCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAAcgcg                                                                      >  1:1226318/1‑140 (MQ=255)

                                                                  tCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGt                                                                    >  1:1199421/1‑140 (MQ=255)

                                                                     ctctGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGa                                                                 <  1:979808/140‑1 (MQ=255)

                                                                       ctgctgGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAAc                                                               >  1:278881/1‑140 (MQ=255)

                                                                        tgctgGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACg                                                              <  1:334013/140‑1 (MQ=255)

                                                                        tgctgGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACg                                                              >  2:373111/1‑140 (MQ=255)

                                                                             gctggcCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTATGA‑CTGGCCGGGAAATATTCGTGAGCTGGaaa                                                                           >  2:538247/1‑122 (MQ=255)

                                                                             gctggcCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTATGA‑CTGGCCGGGAAATATTCGTGAGCTGGaaa                                                                           <  1:538247/122‑1 (MQ=255)

                                                                               tggcCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtg                                                       >  1:479605/1‑140 (MQ=255)

                                                                                ggcCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtgg                                                      >  1:420525/1‑140 (MQ=255)

                                                                                 gcCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtggt                                                     >  1:927672/1‑140 (MQ=255)

                                                                                  ccATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAgtggtg                                                    >  2:169876/1‑140 (MQ=255)

                                                                                          tGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGAc                                            <  1:958397/140‑1 (MQ=255)

                                                                                            cAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCg                                          <  1:862751/140‑1 (MQ=255)

                                                                                                gc‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgctg                                                 >  1:692953/1‑129 (MQ=255)

                                                                                                gc‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGtgctg                                                 <  2:692953/129‑1 (MQ=255)

                                                                                                 c‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGaa                                     >  2:315381/1‑140 (MQ=255)

                                                                                                    ttGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAata                                   <  1:957076/140‑1 (MQ=255)

                                                                                                                                   a‑GGTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCg       <  1:324345/140‑1 (MQ=255)

                                                                                                                                      gTTTTACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGAt     <  2:420525/140‑1 (MQ=255)

                                                                                                                                       ttttACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGAtt    >  2:444823/1‑140 (MQ=255)

                                                                                                                                         ttACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGc  <  2:490330/140‑1 (MQ=255)

                                                                                                                                         ttACGCCCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGc  >  1:490330/1‑140 (MQ=255)

ATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGC‑TTTGCCGAGCGTAAT‑CGC‑AAGGC‑GGTAAAA‑GGTTTTACG‑CCCCAGGCGATGGATCTGTTGATTCATTACGA‑CTGGCCGGGAAATATTCGTGAGCTGGAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGC  >  NC_000913/4204209‑4204479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A