Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,307,957	A→G	G174G (GGT→GGC)	alsE ←	D‑allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,307,957	0	A	G	100.0%	155.6 / NA	44	G174G (GGT→GGC)	alsE	D‑allulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/22); total (22/22)

GTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAT  >  NC_000913/4307838‑4308090

gTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTa                                                                                                                   >  2:152369/1‑140 (MQ=255)

  cATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACt                                                                                                                 <  1:11206/140‑1 (MQ=255)

  cATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACt                                                                                                                 <  2:198952/140‑1 (MQ=255)

    tAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTcc                                                                                                               <  2:69527/140‑1 (MQ=255)

      aTTCTCCATGCTTCGTCGATACTTTCCGAACAATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAACTTGCCTGGTTGCAGGAGCCGACCACCTCAATTTCGTACTCCAg                                                                                                             <  2:901272/140‑1 (MQ=255)

           ccATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCtt                                                                                                        >  2:76635/1‑140 (MQ=255)

           ccATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCtt                                                                                                        >  2:727784/1‑140 (MQ=255)

           ccATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCtt                                                                                                        >  2:153193/1‑140 (MQ=255)

           ccATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCtt                                                                                                        >  1:592187/1‑140 (MQ=255)

           ccATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCtt                                                                                                        >  2:1003822/1‑140 (MQ=255)

           ccACGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCtt                                                                                                        >  2:1178790/1‑140 (MQ=255)

             aTGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTtct                                                                                                      >  2:1253634/1‑140 (MQ=255)

                 ttCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGtt                                                                                                  <  2:415074/140‑1 (MQ=255)

                  tcgtcgATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTc                                                                                                 <  2:938754/140‑1 (MQ=255)

                  tcgtcgATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTc                                                                                                 >  1:1259237/1‑140 (MQ=255)

                   cgtcgATATTATCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCa                                                                                                >  2:285658/1‑140 (MQ=255)

                     tcgATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACg                                                                                              >  2:1281646/1‑140 (MQ=255)

                      cgATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGc                                                                                             <  1:1003822/140‑1 (MQ=255)

                      cgATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGc                                                                                             <  1:727784/140‑1 (MQ=255)

                      cgATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGc                                                                                             <  1:76635/140‑1 (MQ=255)

                              tCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCtt                                                                                     <  2:592187/140‑1 (MQ=255)

                                      aTTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCgg                                                                             <  1:1253634/140‑1 (MQ=255)

                                       ttAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGc                                                                            >  2:387046/1‑140 (MQ=255)

                                       ttAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGc                                                                            >  1:382750/1‑140 (MQ=255)

                                           aCAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGt                                                                        <  1:385415/140‑1 (MQ=255)

                                              ggCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTa                                                                     <  2:720265/140‑1 (MQ=255)

                                               gCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTAt                                                                    >  2:1285790/1‑140 (MQ=255)

                                               gCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTAt                                                                    >  2:186427/1‑140 (MQ=255)

                                                ccGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATc                                                                   <  1:1178790/140‑1 (MQ=255)

                                                   gAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAg                                                                <  1:1281646/140‑1 (MQ=255)

                                                   gAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAg                                                                <  2:382750/140‑1 (MQ=255)

                                                        gCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCGttt                                                           <  2:905893/140‑1 (MQ=255)

                                                         ccGACGATAAAGACGTCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTc                                                          >  2:308424/1‑140 (MQ=255)

                                                          cgacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCa                                                         <  2:770168/140‑1 (MQ=255)

                                                           gacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAg                                                        <  1:1146476/140‑1 (MQ=255)

                                                                   aGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAAc                                                >  2:137470/1‑140 (MQ=255)

                                                                         ccGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGaa                                                 >  2:869905/1‑133 (MQ=255)

                                                                         ccGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGaa                                                 <  1:869905/133‑1 (MQ=255)

                                                                         ccGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGt                                          >  2:341111/1‑140 (MQ=255)

                                                                         ccGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGt                                          >  2:118349/1‑140 (MQ=255)

                                                                              ccTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGc                                     <  1:152369/140‑1 (MQ=255)

                                                                                tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCaa                                   >  2:455129/1‑140 (MQ=255)

                                                                                         cAGTTTTTCGTAAGTTGCCTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGAt                          <  1:186427/140‑1 (MQ=255)

                                                                                                          ccTGGTTGCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTa         >  2:1024339/1‑140 (MQ=255)

                                                                                                                 gCAGGAGCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAt  >  1:473143/1‑140 (MQ=255)

GTCATAATTCTCCATGCTTCGTCGATATTTTCCGCATGATTAAACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAT  >  NC_000913/4307838‑4308090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A