Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,308,118	C→T	A121T (GCC→ACC)	alsE ←	D‑allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,308,118	0	C	T	100.0%	194.2 / NA	54	A121T (GCC→ACC)	alsE	D‑allulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/21); total (33/21)

TCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCC  >  NC_000913/4307988‑4308251

tctcGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTgg                                                                                                                              <  1:387046/140‑1 (MQ=255)

 ctcGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGc                                                                                                                             >  1:1208938/1‑140 (MQ=255)

 ctcGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGc                                                                                                                             >  2:26567/1‑140 (MQ=255)

 ctcGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGc                                                                                                                             <  1:308424/140‑1 (MQ=255)

      tCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGtctc                                                                                                                        <  2:879198/140‑1 (MQ=255)

      tCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGtctc                                                                                                                        <  2:1105705/140‑1 (MQ=255)

      tCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGtctc                                                                                                                        <  1:546485/140‑1 (MQ=255)

      tCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGtctc                                                                                                                        <  1:26567/140‑1 (MQ=255)

       cACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTcc                                                                                                                       >  1:467896/1‑140 (MQ=255)

             aTGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTa                                                                                                                 <  2:142646/140‑1 (MQ=255)

                        tCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGccc                                                                                                      <  2:701197/140‑1 (MQ=255)

                         cGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGcccc                                                                                                     <  2:1208938/140‑1 (MQ=255)

                            cAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGACAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGccc                                                                                                      <  2:355593/136‑1 (MQ=255)

                            cAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGACAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGccc                                                                                                      >  1:355593/1‑136 (MQ=255)

                                        gCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGa                                                                                      <  2:1241502/140‑1 (MQ=255)

                                         cATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAc                                                                                     >  1:1221526/1‑140 (MQ=255)

                                                    tGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTtca                                                                          >  2:1198532/1‑140 (MQ=255)

                                                      aaCGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTtcatc                                                                        >  2:559949/1‑140 (MQ=255)

                                                           ttGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCatcaatca                                                                   <  2:1221526/140‑1 (MQ=255)

                                                             gTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAgg                                                                 >  2:845769/1‑140 (MQ=255)

                                                                cGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCgg                                                              <  1:1285790/140‑1 (MQ=255)

                                                                                 aCAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGAt                                             >  2:770205/1‑140 (MQ=255)

                                                                                  cAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATg                                            >  2:1072772/1‑140 (MQ=255)

                                                                                       aTGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTc                                       <  1:1065735/140‑1 (MQ=255)

                                                                                        tGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTcc                                      >  1:493971/1‑140 (MQ=255)

                                                                                          aCCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCgg                                    >  1:1080575/1‑140 (MQ=255)

                                                                                            cGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGAt                                  <  1:1072772/140‑1 (MQ=255)

                                                                                             gTAATTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATg                                 <  1:559949/140‑1 (MQ=255)

                                                                                               aaTTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCa                               >  2:376950/1‑140 (MQ=255)

                                                                                               aaTTTTATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCa                               >  1:900290/1‑140 (MQ=255)

                                                                                                 ttttATCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCaga                             <  2:900290/140‑1 (MQ=255)

                                                                                                      tCGGCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGtgat                        >  1:346483/1‑140 (MQ=255)

                                                                                                         gCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCTGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGaa                     >  2:795369/1‑140 (MQ=255)

                                                                                                         gCCTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGaa                     >  1:99640/1‑140 (MQ=255)

                                                                                                          ccTTATGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGaaa                    >  1:854664/1‑140 (MQ=255)

                                                                                                              aTGGATATAGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTg                <  2:467896/140‑1 (MQ=255)

                                                                                                                    ataGTATTTCATGGTCTCAATTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCg          >  1:217116/1‑140 (MQ=255)

                                                                                                                    ataGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGtgatg                       >  1:787362/1‑127 (MQ=255)

                                                                                                                    ataGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGtgatg                       <  2:787362/127‑1 (MQ=255)

                                                                                                                      aGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCg          <  1:309808/138‑1 (MQ=255)

                                                                                                                      aGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCg          >  2:309808/1‑138 (MQ=255)

                                                                                                                      aGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCa        >  2:231397/1‑140 (MQ=255)

                                                                                                                      aGTATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCa        >  2:539033/1‑140 (MQ=255)

                                                                                                                        tATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCAcg      >  1:1025191/1‑140 (MQ=255)

                                                                                                                        tATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCAcg      >  1:956876/1‑140 (MQ=255)

                                                                                                                        tATTTCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCAcg      >  2:1093599/1‑140 (MQ=255)

                                                                                                                          tttCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCAcgcg    >  2:764564/1‑140 (MQ=255)

                                                                                                                           ttCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACgcgc   >  2:778565/1‑140 (MQ=255)

                                                                                                                           ttCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACgcgc   >  2:355114/1‑140 (MQ=255)

                                                                                                                           ttCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCATTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACgcgc   >  1:1288321/1‑140 (MQ=255)

                                                                                                                            tCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGcc  >  1:747077/1‑140 (MQ=255)

                                                                                                                            tCATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGcc  >  1:921645/1‑140 (MQ=255)

                                                                                                                             cATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAAt                   >  1:212419/1‑122 (MQ=255)

                                                                                                                             cATGGTCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAAt                   <  2:212419/122‑1 (MQ=255)

TCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTCATCAATCAGGCGGAACGCCTGGCCGTTGATGGTTTCCGGATGCAGAGTGATGAAATCTGCTCCCGCACGCGCC  >  NC_000913/4307988‑4308251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A