Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,309,080	Δ1 bp	coding (390/981 nt)	alsC ←	D‑allose ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,309,077	0	C	.	100.0%	173.9 / NA	41	coding (393/981 nt)	alsC	D‑allose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (13/28); total (13/28)

GTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATACCGGAAAGC  >  NC_000913/4308941‑4309206

gTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcccg                                                                                                                                <  2:173890/140‑2 (MQ=255)

gTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcccg                                                                                                                                >  2:100570/1‑139 (MQ=255)

gTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcccg                                                                                                                                <  1:818582/140‑2 (MQ=255)

gTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcccg                                                                                                                                <  1:584346/140‑2 (MQ=255)

    cccAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCa                                                                                                                           <  1:886922/140‑1 (MQ=255)

     ccAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAg                                                                                                                          <  1:45022/140‑1 (MQ=255)

             gCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcccgtc                                                                                                                              <  2:463958/129‑4 (MQ=255)

             gCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGcccgtc                                                                                                                              >  1:463958/1‑126 (MQ=255)

              cTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAgg                                                                                                                 <  2:149759/140‑1 (MQ=255)

              cTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAgg                                                                                                                 <  2:1061882/140‑1 (MQ=255)

              cTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAgg                                                                                                                 <  1:72734/140‑1 (MQ=255)

                   ggcAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGcc                                                                                                            >  1:1039649/1‑140 (MQ=255)

                   ggcAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGcc                                                                                                            <  1:1077523/140‑1 (MQ=255)

                            gTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGc                                                                                                   <  1:1016533/140‑1 (MQ=255)

                               cACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGAt                                                                                                      <  1:1033568/134‑1 (MQ=255)

                               cACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGAt                                                                                                      >  2:1033568/1‑134 (MQ=255)

                                         aaTGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGcgccg                                                                                      <  1:406586/140‑1 (MQ=255)

                                         aaTGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGcgccg                                                                                      <  1:100570/140‑1 (MQ=255)

                                         aaTGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGcgccg                                                                                      <  1:342345/140‑1 (MQ=255)

                                         aaTGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGcgccg                                                                                      <  1:581824/140‑1 (MQ=255)

                                         aaTGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGcgccg                                                                                      <  2:1039649/140‑1 (MQ=255)

                                           tGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCgccgcc                                                                                    <  1:1128014/140‑1 (MQ=255)

                                                aGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAAcc                                                                                <  1:1054945/139‑1 (MQ=255)

                                                aGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAAcc                                                                                >  2:1054945/1‑139 (MQ=255)

                                                   cGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTa                                                                            <  1:323786/140‑1 (MQ=255)

                                                           gAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCa                                                                    >  2:55218/1‑140 (MQ=255)

                                                            aGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTaca                                                                          <  2:338743/133‑1 (MQ=255)

                                                            aGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTaca                                                                          >  1:338743/1‑133 (MQ=255)

                                                            aGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCaa                                                                   >  1:881873/1‑140 (MQ=255)

                                                                            cACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGaaa                                                   <  2:881873/140‑1 (MQ=255)

                                                                            cACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGaaa                                                   <  2:132970/140‑1 (MQ=255)

                                                                               cAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACgg                                                >  2:273227/1‑140 (MQ=255)

                                                                               cAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACgg                                                <  1:389972/140‑1 (MQ=255)

                                                                                 gCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGt                                              <  1:55218/140‑1 (MQ=255)

                                                                                 gCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGt                                              <  2:775601/140‑1 (MQ=255)

                                                                                      aTCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAaca                                         >  1:1070507/1‑140 (MQ=255)

                                                                                      aTCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAaca                                         >  1:342558/1‑140 (MQ=255)

                                                                                      aTCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAaca                                         >  1:379215/1‑140 (MQ=255)

                                                                                      aTCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTACGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAaca                                         >  2:1059971/1‑140 (MQ=255)

                                                                                       tCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAacac                                        <  2:388171/140‑1 (MQ=255)

                                                                                            cGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTACGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGcc                                   <  1:1059971/140‑1 (MQ=255)

                                                                                                             gCCAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGt                  <  1:860043/140‑1 (MQ=255)

                                                                                                               cAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCGAACATCAGTTTGGCGGTCa                <  1:745482/140‑1 (MQ=255)

                                                                                                               cAAGGGTGATGATGAACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCa                <  2:1070507/140‑1 (MQ=255)

                                                                                                                      gatgatgaACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATAcc         >  1:587479/1‑140 (MQ=255)

                                                                                                                          atgaACGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATACCGGaa     <  2:587479/140‑1 (MQ=255)

                                                                                                                             aaCGGGTGTAG‑CCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATACCGGAAAgc  >  1:165356/1‑140 (MQ=255)

GTATCCCAATTACGCTGGCGGCAAAGAAGTTCACGAAGTCAAATGAGAAGCCGTATACCGAGTTGGCATCGGAGATCACCAGCGTGATCCCACGGAAAATCGCGTTGGTGCCAAGGGTGATGATGAACGGGTGTAGCCCCGTCCAGTTGACCAGGCAGCCGTTGATCGCCCCCAGTGCGCCGCCAACCAGTACACCGCCAATCATCGCTGCGAGAAACGGGTCAACACCTGCCAACATCAGTTTGGCGGTCACCATACCGGAAAGC  >  NC_000913/4308941‑4309206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A