Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,518,258	A→G	V25A (GTG→GCG)	ydcZ ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,518,258	0	A	G	100.0%	126.3 / NA	36	V25A (GTG→GCG)	ydcZ	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/16); total (20/16)

CGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCC  >  NC_000913/1518123‑1518392

cgcACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCAt                                                                                                                                    >  2:514452/1‑140 (MQ=255)

    cGCTGGACCCCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGc                                                                                                                                <  2:573400/140‑1 (MQ=255)

    cGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGc                                                                                                                                <  1:242906/140‑1 (MQ=255)

    cGCTGGACACCAGATCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGc                                                                                                                                >  2:258651/1‑140 (MQ=255)

       tGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCgtg                                                                                                                             >  2:42077/1‑140 (MQ=255)

               aGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGAc                                                                                                                     >  2:62362/1‑140 (MQ=255)

                   cGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAAcc                                                                                                                 >  1:908398/1‑140 (MQ=255)

                   cGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAAcc                                                                                                                 >  2:291584/1‑140 (MQ=255)

                                    ccTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAAc                                                                                                                  <  2:841495/122‑1 (MQ=255)

                                    ccTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAAc                                                                                                                  >  1:841495/1‑122 (MQ=255)

                                          ttAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCaa                                                                                          <  1:291584/140‑1 (MQ=255)

                                                cATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGc                                                                                    <  1:2355/140‑1 (MQ=255)

                                                       tAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTg                                                                             <  1:258651/140‑1 (MQ=255)

                                                            aCAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTg                                                                             >  1:922637/1‑135 (MQ=255)

                                                            aCAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTg                                                                             <  2:922637/135‑1 (MQ=255)

                                                                     acaATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCAt                                                               <  2:636994/140‑1 (MQ=255)

                                                                          ccccACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTCGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAAt                                                          <  2:908398/140‑1 (MQ=255)

                                                                                caGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTc                                                    <  1:62362/140‑1 (MQ=255)

                                                                                   tGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCgg                                                 <  1:875132/140‑1 (MQ=255)

                                                                                   tGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCgg                                                 >  1:107356/1‑140 (MQ=255)

                                                                                                 tGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCtcgt                                   >  1:18305/1‑140 (MQ=255)

                                                                                                           aaTGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCaa                         >  2:213260/1‑140 (MQ=255)

                                                                                                           aaTGGTAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCaa                         >  2:425951/1‑140 (MQ=255)

                                                                                                                tAGAGGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCa                    <  1:514452/140‑1 (MQ=255)

                                                                                                                 agagGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCAt                   <  1:425951/140‑1 (MQ=255)

                                                                                                                 agagGAGGTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCAt                   <  1:213260/140‑1 (MQ=255)

                                                                                                                       ggTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGt             <  2:33012/140‑1 (MQ=255)

                                                                                                                        gTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCa                          >  1:671022/1‑126 (MQ=255)

                                                                                                                        gTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCa                          <  2:671022/126‑1 (MQ=255)

                                                                                                                        gTCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTc            >  2:857638/1‑140 (MQ=255)

                                                                                                                         tCTGGGTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCa           >  2:577039/1‑140 (MQ=255)

                                                                                                                             ggTGATGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATc       >  1:984482/1‑140 (MQ=255)

                                                                                                                              gTGATGTGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCACCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATcc      >  1:868914/1‑140 (MQ=255)

                                                                                                                                  tGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGcc  >  2:338588/1‑140 (MQ=255)

                                                                                                                                  tGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGcc  >  1:59249/1‑140 (MQ=255)

                                                                                                                                  tGCGCGCCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGcc  >  1:2175/1‑140 (MQ=255)

CGCACGCTGGACACCAGTTCGCCAAACCCCGCCATGCCTTGTTTAAACCATAAAATAGAAACAAAGAGCACAATCCCCACCAGTGAGTTCAGCAACATGGCGATGAGAATGGTAGAGGAGGTCTGGGTGATGCGCACCATCAGCGTGTTCTGGACAACCAGACCAATTCCGGCGGCAATCAAAAAAGCAAGGGTGAGCGACTGATTCATCCAATCGCGTCCGGTTCAGTGCGCTCGTCGAGTTGCAACTGCATAAATGTCAGATCCAGCC  >  NC_000913/1518123‑1518392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A