Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,546,404	A→G	S256P (TCC→CCC)	yddG ←	aromatic amino acid exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,546,404	0	A	G	100.0%	112.2 / NA	36	S256P (TCC→CCC)	yddG	aromatic amino acid exporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/22); total (14/22)

GTAGACCCGGCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTG  >  NC_000913/1546270‑1546534

gTAGACCCGGCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTa                                                                                                                               <  2:663830/140‑1 (MQ=255)

gTAGACCCGGCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTa                                                                                                                               <  2:729576/140‑1 (MQ=255)

 tAGACCCGGCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTAc                                                                                                                              >  2:309771/1‑140 (MQ=255)

        ggCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTa                                                                                                                       <  2:192161/140‑1 (MQ=255)

        ggCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTa                                                                                                                       <  1:191132/140‑1 (MQ=255)

        ggCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTa                                                                                                                       >  2:817434/1‑140 (MQ=255)

         gCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTaa                                                                                                                      <  2:671115/140‑1 (MQ=255)

               aTTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACg                                                                                                                <  1:309771/140‑1 (MQ=255)

                     aCCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACg                                                                                                                >  1:116009/1‑134 (MQ=255)

                     aCCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACg                                                                                                                <  2:116009/134‑1 (MQ=255)

                               gtCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTATAATACGAACCTACCGCCATAAt                                                                                                >  1:907651/1‑140 (MQ=255)

                                      cccacaagaggagGAAC‑CCGCCGCAGACCACCAGCGCGCCTTGCCAGAAGGAGAACGACAGGGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACa                                                                                         <  2:634312/134‑1 (MQ=255)

                                       ccagcAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACAt                                                                                        >  1:562681/1‑140 (MQ=255)

                                         agcagagcagGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTCCCGCCATAATGGTGACATTg                                                                                      <  1:492130/140‑1 (MQ=255)

                                            aagggcgggAACCCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGcc                                                                                    <  1:935423/134‑1 (MQ=255)

                                              gggggggAC‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATg                                                                                 <  2:91330/136‑1 (MQ=255)

                                                 agGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCaa                                                                              >  2:46392/1‑140 (MQ=255)

                                                    gAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAatat                                                                           >  2:312043/1‑140 (MQ=255)

                                                           ccgcAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGAc                                                                     <  1:280023/140‑1 (MQ=255)

                                                              cAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACAtt                                                                  >  1:177287/1‑140 (MQ=255)

                                                                 aCCATCAGCGCCCCTTGCAAGAAGGAGAACGACAGGGGGGCGCGGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGACCCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCa                                                               <  2:257843/140‑1 (MQ=255)

                                                                   caccAGCGCGCCTTGCCAGAACGAGAACGCCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGACCCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATg                                                             <  2:275219/137‑1 (MQ=255)

                                                                   cATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATg                                                             <  2:177287/140‑1 (MQ=255)

                                                                      cAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATgcag                                                          >  1:257843/1‑140 (MQ=255)

                                                                             ccTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGc                                                   <  1:111435/140‑1 (MQ=255)

                                                                             ccTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGAAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGc                                                   <  2:273734/140‑1 (MQ=255)

                                                                                    agaacgagaacgACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCt                                            <  1:46392/140‑1 (MQ=255)

                                                                                        cgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCAt                                                       >  1:380671/1‑125 (MQ=255)

                                                                                        cgagaacgaCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCAt                                                       <  2:380671/125‑1 (MQ=255)

                                                                                                aCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTaaa                                    <  2:987390/136‑1 (MQ=255)

                                                                                                aCAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTaaa                                    >  1:987390/1‑136 (MQ=255)

                                                                                                    cGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCag                            >  2:184346/1‑140 (MQ=255)

                                                                                                        gcgcTGAGCAGCACGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGAt                        <  1:312043/140‑1 (MQ=255)

                                                                                                               gcagcaCGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTa                 <  1:184346/140‑1 (MQ=255)

                                                                                                                     cGGCTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATg           >  1:982418/1‑140 (MQ=255)

                                                                                                                        cTGCAAGCGCTGAGGGAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACg        >  1:635140/1‑140 (MQ=255)

                                                                                                                           cAAGCGCTGAGGGAAGTACAGGCGTAAAATACGACCCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGc     <  1:817434/140‑1 (MQ=255)

                                                                                                                              gcgcTGAGGGAAGTACAGGCGAAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTg  <  1:599079/140‑1 (MQ=255)

GTAGACCCGGCAGTTATTTTAACCACGACGTGTCGCCAGCCAGCAGAGCAGGGAA‑CCGCCGCAGACCATCAGCGCGCCTTGCCAGAACGAGAACGACAGCGGGGCGCTGAGCAGCACGGCTGCAAGCGCTGAGGAAAGTACAGGCGTAAAATACGAACCTACCGCCATAATGGTGACATTGCCATGCAATATACCGACATTCCATGCAGCATAAGCAAATCCTAAGGTAAATGCCGCAGAGATGAGTTTAATCATGACGGGCGTG  >  NC_000913/1546270‑1546534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A