Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,547,482	C→T	P28S (CCG→TCG)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,547,482	0	C	T	100.0%	159.3 / NA	46	P28S (CCG→TCG)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/22); total (24/22)

TTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGGCGCAAAAAACGCCAGAG  >  NC_000913/1547357‑1547617

ttAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTg                                                                                                                           >  2:110265/1‑140 (MQ=255)

            cgcAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGa                                                                                                               >  2:773811/1‑140 (MQ=255)

             gcAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGaa                                                                                                              >  1:744342/1‑140 (MQ=255)

                    tACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACaa                                                                                                       <  2:211848/140‑1 (MQ=255)

                         cTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTa                                                                                                          <  1:511057/132‑1 (MQ=255)

                         cTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTa                                                                                                          >  2:511057/1‑132 (MQ=255)

                         cTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAattat                                                                                                  >  2:493373/1‑140 (MQ=255)

                         cTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAattat                                                                                                  >  2:92021/1‑140 (MQ=255)

                           gAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGgcgc                                                                                                                 <  1:452866/123‑1 (MQ=255)

                           gAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGgcgc                                                                                                                 >  2:452866/1‑123 (MQ=255)

                           gAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAAttatta                                                                                                >  2:70465/1‑140 (MQ=255)

                           gAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAAttatta                                                                                                >  2:735876/1‑140 (MQ=255)

                           gAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAAttatta                                                                                                >  2:164264/1‑140 (MQ=255)

                           gAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAAttatta                                                                                                >  2:136682/1‑140 (MQ=255)

                            aaaaaaGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTAc                                                                                               <  1:773811/140‑1 (MQ=255)

                             aaaaaGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTAcg                                                                                              <  1:92021/140‑1 (MQ=255)

                             aaaaaGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTAcg                                                                                              <  2:744342/140‑1 (MQ=255)

                               aaaGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTAcgcg                                                                                            <  1:182793/140‑1 (MQ=255)

                               aaaGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTAcgcg                                                                                            <  1:70465/140‑1 (MQ=255)

                                    ggAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTaaa                                                                                       >  2:817913/1‑140 (MQ=255)

                                      aaaGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTAcg                                                                                              <  2:510099/131‑1 (MQ=255)

                                      aaaGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTAcg                                                                                              >  1:510099/1‑131 (MQ=255)

                                             tGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTa                                                                                         <  1:457857/129‑1 (MQ=255)

                                             tGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTa                                                                                         >  2:457857/1‑129 (MQ=255)

                                                 cGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAaca                                                                          <  1:110265/140‑1 (MQ=255)

                                                 cGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAaca                                                                          <  1:735876/140‑1 (MQ=255)

                                                 cGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAaca                                                                          <  1:136682/140‑1 (MQ=255)

                                                    cAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCt                                                                       >  1:362027/1‑140 (MQ=255)

                                                      gTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGc                                                                     >  1:707925/1‑140 (MQ=255)

                                                      gTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGc                                                                     <  1:164264/140‑1 (MQ=255)

                                                       tCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGca                                                                    >  1:694738/1‑140 (MQ=255)

                                                         gCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGcaca                                                                  >  1:739560/1‑140 (MQ=255)

                                                                         aaTCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGtt                                                  <  2:201431/140‑1 (MQ=255)

                                                                          aTCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTg                                                 <  2:694738/140‑1 (MQ=255)

                                                                               cgcgGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGc                                            <  2:66181/140‑1 (MQ=255)

                                                                               cgcgGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGc                                            <  1:653538/140‑1 (MQ=255)

                                                                                 cgggcggTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTa                                          <  2:362027/140‑1 (MQ=255)

                                                                                   ggcggTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTAtt                                        <  2:707925/140‑1 (MQ=255)

                                                                                   ggcggTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTAtt                                        <  1:817913/140‑1 (MQ=255)

                                                                                                           gCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGgcgc                >  1:282279/1‑140 (MQ=255)

                                                                                                           gCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGgcgc                >  1:732733/1‑140 (MQ=255)

                                                                                                           gCGGCATTGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGGGATGgcgc                >  1:696699/1‑140 (MQ=255)

                                                                                                                 ttGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGGCGCaaaaaa          >  2:684062/1‑140 (MQ=255)

                                                                                                                 ttGGGCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGGCGCaaaaaa          <  2:739560/140‑1 (MQ=255)

                                                                                                                    ggCTTTGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGGCGCAAAAAACGc       >  1:13480/1‑140 (MQ=255)

                                                                                                                         tGCCTCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGGCGCAAAAAACGCCagag  >  2:988888/1‑140 (MQ=255)

TTAGTTTCCTCGCGCAGTAATACCCCTGAAAAAAGAGGAAAGCAATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACAGTAGCGGCATTGGGCTTTGCCCCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTATTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTGATGTATAGCCTGGGTGATGGCGCAAAAAACGCCAGAG  >  NC_000913/1547357‑1547617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A