Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	155	60.3	2039550	99.3%	2025273	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,554,800	A→G	G290G (GGT→GGC)	maeA ←	malate dehydrogenase, NAD‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,554,800	0	A	G	100.0%	169.0 / NA	50	G290G (GGT→GGC)	maeA	malate dehydrogenase, NAD‑requiring
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/27); total (23/27)

TCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCA  >  NC_000913/1554663‑1554935

tCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGaccgcc                                                                                                                                       >  2:215218/1‑140 (MQ=255)

  gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTg                                                                                                                                     <  2:632321/140‑1 (MQ=255)

            aCTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCt                                                                                                                           >  2:758104/1‑140 (MQ=255)

            aCTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCt                                                                                                                           >  1:720323/1‑140 (MQ=255)

              tttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTtg                                                                                                                         >  2:598654/1‑140 (MQ=255)

              tttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTtg                                                                                                                         >  1:926550/1‑140 (MQ=255)

              tttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACCGACCGCCTGCCGCGCGGc                                                                                                                            <  1:179880/137‑1 (MQ=255)

              tttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACCGACCGCCTGCCGCGCGGc                                                                                                                            >  2:179880/1‑137 (MQ=255)

               ttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCcgcgcg                                                                                                                              <  2:885471/134‑1 (MQ=255)

               ttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCcgcgcg                                                                                                                              >  1:885471/1‑134 (MQ=255)

               ttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTtgc                                                                                                                        <  2:1002728/140‑1 (MQ=255)

                tCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTtgct                                                                                                                       <  2:720323/140‑1 (MQ=255)

                    ccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGa                                                                                                                   <  1:867498/140‑1 (MQ=255)

                                            ttCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgccg                                                                                           >  2:545810/1‑140 (MQ=255)

                                            ttCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgccg                                                                                           >  1:151354/1‑140 (MQ=255)

                                              cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGCCCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                         <  2:151354/140‑1 (MQ=255)

                                              cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                         <  1:916670/140‑1 (MQ=255)

                                              cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                         <  1:63279/140‑1 (MQ=255)

                                              cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                         >  1:603760/1‑140 (MQ=255)

                                              cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                         <  2:409408/140‑1 (MQ=255)

                                              cgcgCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                                         >  2:63279/1‑140 (MQ=255)

                                                   tGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGcc                                                                                    <  2:78973/140‑1 (MQ=255)

                                                   tGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGcc                                                                                    <  1:545810/140‑1 (MQ=255)

                                                   tGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGcc                                                                                    <  1:939475/140‑1 (MQ=255)

                                                    gggTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGccc                                                                                   <  2:926550/140‑1 (MQ=255)

                                                               atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATg                                                                             <  2:635584/135‑1 (MQ=255)

                                                               atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATg                                                                             >  1:635584/1‑135 (MQ=255)

                                                               atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATc                                                                        >  1:654099/1‑140 (MQ=255)

                                                               atgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATc                                                                        >  1:492295/1‑140 (MQ=255)

                                                                tgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCg                                                                       >  1:492287/1‑140 (MQ=255)

                                                                 gatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGt                                                                      >  2:445779/1‑140 (MQ=255)

                                                                      tttCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTaaaa                                                                 >  1:407054/1‑140 (MQ=255)

                                                                      tttCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTaaaa                                                                 >  1:158446/1‑140 (MQ=255)

                                                                          ggCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGTCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAAc                                                             >  1:194526/1‑140 (MQ=255)

                                                                                gCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                                       <  2:158446/140‑1 (MQ=255)

                                                                                gCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                                       <  2:407054/140‑1 (MQ=255)

                                                                                 ccGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTc                                                      >  2:127473/1‑140 (MQ=255)

                                                                                         cGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                              <  2:654099/140‑1 (MQ=255)

                                                                                          gCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATa                                             <  2:492287/140‑1 (MQ=255)

                                                                                           cTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAg                                            >  1:956897/1‑140 (MQ=255)

                                                                                           cTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAg                                            >  1:124303/1‑140 (MQ=255)

                                                                                            tGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGc                                           <  1:127473/140‑1 (MQ=255)

                                                                                              aaCCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                              <  2:742968/135‑1 (MQ=255)

                                                                                              aaCCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                              >  1:742968/1‑135 (MQ=255)

                                                                                               aCCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGt                                        <  1:567594/140‑1 (MQ=255)

                                                                                                          ggAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                              >  1:996751/1‑123 (MQ=255)

                                                                                                          ggAAGACGATTTTTTTCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                              <  2:996751/123‑1 (MQ=255)

                                                                                                                    ttttttCTCGCTTAACTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAtttt                   <  2:999894/140‑1 (MQ=255)

                                                                                                                                  aCTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCt     <  2:124303/140‑1 (MQ=255)

                                                                                                                                  aCTGACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCt     <  2:956897/140‑1 (MQ=255)

                                                                                                                                     gACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa  <  1:823967/140‑1 (MQ=255)

                                                                                                                                     gACCGCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCa  <  1:295771/140‑1 (MQ=255)

TCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGAGCAAAGTCTTCA  >  NC_000913/1554663‑1554935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A